Difference between revisions of "Team:UST Beijing/InterLab"

 
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    <li><a href="https://2017.igem.org/Team:UST_Beijing/Attributions">Attributions</a></li>
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                  <li><a href="https://2017.igem.org/Team:UST_Beijing/InterLab">InterLab</a></li>
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</ul>
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<div class="container" align="center">
 
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    <h2>Wiki of InterLab by UST_Beijing</h2><br>
 
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          <h4 class="panel-title"><h3>Background</h3></h4>
 
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          <div class="panel-body">
 
−
          <p class="text-justify" align="left">
 
−
          “All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.
 
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        <div class="panel-heading">
 
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          <h4 class="panel-title"><h3>Materials & Methods</h3></h4>
 
−
        </div>
 
−
        <div id="Abstract" class="panel-collapse collapse in">
 
−
          <div class="panel-body">
 
−
          <h4  style="border-bottom:1px solid black;  padding-top:6px;padding-bottom: 12px">Plasmids used</h4>
 
−
            <p class="text-justify" style="text-align:center" >
 
−
Kit Plate 7 <br/>
 
  
−
Positive Control (BBa_I20270): well 21B<br/>
 
−
Negative Control (BBa_R0040): well 21D<br/>
 
−
Test Device 1 (BBa_J364000): well 21F<br/>
 
−
Test Device 2 (BBa_J364001): well 21H<br/>
 
−
Test Device 3 (BBa_J364002): well 21J<br/>
 
−
Test Device 4 (BBa_J364003): well 21L<br/>
 
−
Test Device 5 (BBa_J364004): well 21N<br/>
 
−
Test Device 6 (BBa_J364005): well 21P<br/>
 
−
<br/>
 
−
          </p>
 
−
              <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Strain used</h4>
 
−
            <p class="text-justify" style="text-align:center" >
 
  
−
Escherichia coli DH5α <br/>
+
<section style="background-image:url(https://static.igem.org/mediawiki/2017/f/f8/Ustbbjbj.png);background-position:center center;background-repeat:no-repeat;-moz-background-size: cover;background-size: cover;background-attachment:fixed;">
 +
 
  
  
 +
<div class="container" align="center">
 +
    <br /><br /><br />
 +
          <h2 style="font-family:Arial">InterLab Measurement Study</h2><br />
 +
          <p class="text-justify" style="width:700px;text-indent:1em; text-align: left;font-size:18px;line-height:28px;font-family:Arial">
 +
          “All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.
 +
      </p>
 +
        <br /><br /><br /><br /><br /><br />
 +
          <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Plasmids used</p>
 +
          <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
Kit Plate 7 <br />
 +
Positive Control (BBa_I20270): well 21B<br />
 +
Negative Control (BBa_R0040): well 21D<br />
 +
Test Device 1 (BBa_J364000): well 21F<br />
 +
Test Device 2 (BBa_J364001): well 21H<br />
 +
Test Device 3 (BBa_J364002): well 21J<br />
 +
Test Device 4 (BBa_J364003): well 21L<br />
 +
Test Device 5 (BBa_J364004): well 21N<br />
 +
Test Device 6 (BBa_J364005): well 21P<br />
 +
<br />
 
           </p>
 
           </p>
−
              <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Materials</h4>
+
        <br /><br /><br />
−
            <p class="text-justify" style="text-align:center" >
+
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Strain used</p>
−
FITC Standard: one tube with dried down FITC for creating a FITC standard <br/>
+
        <br />
−
• LUDOX: one tube with 30% colloidal silica suspended in 1mL of water <br/>
+
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
−
• 1xPBS (phosphate buffered saline) <br/>
+
        Escherichia coli DH5α<br />
−
• LB (Luria Bertani) media <br/>
+
        <br />
−
• Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH) <br/>
+
        </p>
−
• 50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth <br/>
+
        <br /><br /><br />
−
• 1.5 ml eppendorf tubes for sample storage <br/>
+
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Materials</p>
−
• Ice bucket with ice <br/>
+
        <br />
−
• Pipettes <br/>
+
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
−
• 96 well plate  <br/>
+
FITC Standard: one tube with dried down FITC for creating a FITC standard <br />
−
  <br/>
+
LUDOX: one tube with 30% colloidal silica suspended in 1mL of water <br />
 +
1xPBS (phosphate buffered saline) <br />
 +
LB (Luria Bertani) media <br />
 +
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH) <br />
 +
50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth <br />
 +
1.5 ml eppendorf tubes for sample storage <br />
 +
Ice bucket with ice <br />
 +
Pipettes <br />
 +
96 well plate  <br />
 +
  <br />
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Machines</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        Enspire 2300-001M<br />
 +
        Sigma Laborzentrifugen<br />
 +
        Thermo Scientific Nanodrop 2000<br />
 +
        EVOS AMG microscope<br />
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Software</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        Microsoft Excel<br />
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Methods</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        Calibration<br />
 +
        OD600 Reference point<br />
 +
        FITC fluorescence standard curve<br />
 +
        Test Device 1 (BBa_J364000): well 21F<br />
 +
        Test Device 2 (BBa_J364001): well 21H<br />
 +
        Test Device 3 (BBa_J364002): well 21J<br />
 +
        Test Device 4 (BBa_J364003): well 21L<br />
 +
        Test Device 5 (BBa_J364004): well 21N<br />
 +
        Test Device 6 (BBa_J364005): well 21P<br />
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Cell measurement</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial">
 +
        Transformation<br />
 +
        Measurements<br />
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Protocols</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        <a href="http://parts.igem.org/Help:Protocols/Transformation" style="color:blue">Please click here</a>
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br /><br /><br /><br />
 +
        <object width="70%" height="800px" data="https://static.igem.org/mediawiki/2017/e/e8/Wiki_of_InterLab_by_UST_Beijing.pdf">  
 +
        </object>
 +
</div>
 +
  <br /><br /><br /><br />
  
 +
    <div class="container" align="center">
 +
        <p align="center" style="font-size: 5px"><br><h5>Copyright © 2017 UST_Beijing iGEM. All rights reserved.</h5></p>
 +
    </div>
 +
   
 +
</section>
  
 +
</body>
  
 +
<style>
 +
  ul ul {
 +
    background:#E4EAF1;
 +
    border-radius:0;
 +
    position:absolute;
 +
    top:100%;
 +
    padding:0;
  
−
          </p>
+
}
−
<h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Machines</h4>
+
ul  {
−
            <p class="text-justify" style="text-align:center" >
+
    float:none;
 +
    border-top:1px solid #6b727c;
 +
    border-bottom:1px solid #575f6a;
  
−
Enspire 2300-001M<br/>
 
−
Sigma Laborzentrifugen<br/>
 
−
Thermo Scientific Nanodrop 2000<br/>
 
−
EVOS AMG microscope<br/>
 
  
 +
}
 +
body{
 +
font-family:Arial;
 +
}
 +
.scroll-link{
 +
font-size: 14px;
 +
}
 +
ul ul li a {
 +
    color:#FFF;
  
−
          </p>
+
}
−
              <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Software</h4>
+
−
            <p class="text-justify" style="text-align:center" >
+
  
−
Microsoft Excel<br/>
+
ul ul li a:hover {
 +
    background:#FFFFFF;
  
 +
}
  
−
          </p>
+
ul ul ul {
 +
width: 100%;
 +
position: absolute;
 +
left: 100%;
 +
top: 50%;
 +
}
 +
</style>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy1').hide(); //打开页面隐藏下拉列表
 +
$('#cs1').hover( //鼠标滑过导航栏目时
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$('#csy1').show(); //显示下拉列表
  
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$('#csy1').hide(); //鼠标移开后隐藏下拉列表
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}
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$('#csy1').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
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 +
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$('#csy1').hide();
  
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$('cs1').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
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});
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy2').hide(); //打开页面隐藏下拉列表
 +
$('#cs2').hover( //鼠标滑过导航栏目时
 +
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 +
$('#csy2').show(); //显示下拉列表
  
 +
},
 +
function(){
 +
$('#csy2').hide(); //鼠标移开后隐藏下拉列表
 +
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 +
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 +
$('#csy2').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
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 +
$('#csy2').show();
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 +
$('#csy2').hide();
  
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$('cs2').hover( //鼠标滑过下拉列表是改变当前栏目样式
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<script type="text/javascript">
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$(document).ready(function (){
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$('#csy3').hide(); //打开页面隐藏下拉列表
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$('#cs3').hover( //鼠标滑过导航栏目时
 +
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$('#csy3').show(); //显示下拉列表
  
 +
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$('#csy3').hide(); //鼠标移开后隐藏下拉列表
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              <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Methods</h4>
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function(){
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Calibration<br/>
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},
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OD600 Reference point<br/>
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function(){
−
FITC fluorescence standard curve<br/>
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$('#csy4').hide(); //鼠标移开后隐藏下拉列表
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Test Device 1 (BBa_J364000): well 21F<br/>
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}
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Test Device 2 (BBa_J364001): well 21H<br/>
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);
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Test Device 3 (BBa_J364002): well 21J<br/>
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$('#csy4').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
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Test Device 4 (BBa_J364003): well 21L<br/>
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function(){
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Test Device 5 (BBa_J364004): well 21N<br/>
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$('#csy4').show();
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Test Device 6 (BBa_J364005): well 21P<br/>
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},
 +
function(){
 +
$('#csy4').hide();
  
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          </p>
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function(){
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              <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Cell measurement</h4>
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function(){
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function(){
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Transformation<br/>
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Measurements
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<br/>
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          </p>
 
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            <p class="text-justify" style="text-align:center" >
 
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−
Please click the link:<br/>
 
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<a href="https://2017.igem.org/Competition/InterLab_Study">https://2017.igem.org/Competition/InterLab_Study</a>>
 
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<br/>
 
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Latest revision as of 16:59, 1 November 2017

USTB-Beijing | Welcome




InterLab Measurement Study


“All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.







Plasmids used


Kit Plate 7
Positive Control (BBa_I20270): well 21B
Negative Control (BBa_R0040): well 21D
Test Device 1 (BBa_J364000): well 21F
Test Device 2 (BBa_J364001): well 21H
Test Device 3 (BBa_J364002): well 21J
Test Device 4 (BBa_J364003): well 21L
Test Device 5 (BBa_J364004): well 21N
Test Device 6 (BBa_J364005): well 21P




Strain used


Escherichia coli DH5α




Materials


FITC Standard: one tube with dried down FITC for creating a FITC standard
LUDOX: one tube with 30% colloidal silica suspended in 1mL of water
1xPBS (phosphate buffered saline)
LB (Luria Bertani) media
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH)
50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth
1.5 ml eppendorf tubes for sample storage
Ice bucket with ice
Pipettes
96 well plate




Machines


Enspire 2300-001M
Sigma Laborzentrifugen
Thermo Scientific Nanodrop 2000
EVOS AMG microscope




Software


Microsoft Excel




Methods


Calibration
OD600 Reference point
FITC fluorescence standard curve
Test Device 1 (BBa_J364000): well 21F
Test Device 2 (BBa_J364001): well 21H
Test Device 3 (BBa_J364002): well 21J
Test Device 4 (BBa_J364003): well 21L
Test Device 5 (BBa_J364004): well 21N
Test Device 6 (BBa_J364005): well 21P




Cell measurement


Transformation
Measurements




Protocols


Please click here












Copyright © 2017 UST_Beijing iGEM. All rights reserved.