Difference between revisions of "Team:UST Beijing/InterLab"

 
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  <div class="scroll-link">Project</div></a>
 
  <div class="scroll-link">Project</div></a>
 
  <ul id="csy2">
 
  <ul id="csy2">
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                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Pangu">Pangu</a></li>
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                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Cyclase">Cyclase</a></li>
 
                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Experiments">Glucosidase</a></li>
 
                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Experiments">Glucosidase</a></li>
−
                  <li><a href="https://2017.igem.org/Team:UST_Beijing/InterLab">InterLab</a></li>
 
 
                         </ul>
 
                         </ul>
 
  </li>
 
  </li>
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  <span id="ca-icon4"></span>
 
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  <div class="scroll-link" >Parts</div></a></li>
 
  <div class="scroll-link" >Parts</div></a></li>
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  <li><a id="cs4">
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  <li><a href="https://2017.igem.org/Team:UST_Beijing/Team">
 
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  <div class="scroll-link">Team</div></a>
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  <div class="scroll-link">Team</div></a></li>
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  <ul id="csy4">
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            <li><a href="https://2017.igem.org/Team:UST_Beijing/Attributions">Attributions</a></li>
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                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Team">Members</a></li></ul>
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  </li>
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  <li><a id="cs5">
 
  <li><a id="cs5">
 
  <span id="ca-icon6"></span>
 
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                   <li><a href="https://2017.igem.org/Team:UST_Beijing/InterLab">InterLab</a></li>
 
                   <li><a href="https://2017.igem.org/Team:UST_Beijing/InterLab">InterLab</a></li>
 
                         </ul>
 
                         </ul>
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<section id="aboutUs" style="background-image:url(https://static.igem.org/mediawiki/2017/f/f8/Ustbbjbj.png);background-position:center center;background-repeat:no-repeat;-moz-background-size: cover;background-size: cover;background-attachment:fixed;">
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<section style="background-image:url(https://static.igem.org/mediawiki/2017/f/f8/Ustbbjbj.png);background-position:center center;background-repeat:no-repeat;-moz-background-size: cover;background-size: cover;background-attachment:fixed;">
 
    
 
    
  
  
 
<div class="container" align="center">
 
<div class="container" align="center">
−
    <h2>Wiki of InterLab by UST_Beijing</h2><br>
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    <br /><br /><br />
−
    <div class="panel-group" id="BackgroundContent" role="tablist" aria-multiselectable="true">
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           <h2 style="font-family:Arial">InterLab Measurement Study</h2><br />
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      <div class="panel panel-transparent">
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           <p class="text-justify" style="width:700px;text-indent:1em; text-align: left;font-size:18px;line-height:28px;font-family:Arial">
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        <div class="panel-heading" role="tab">
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−
           <h4 class="panel-title"><h3>Background</h3></h4>
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        </div>
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−
        <div id="Background" class="panel-collapse collapse in">
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−
           <div class="panel-body">
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          <p class="text-justify" align="left">
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           “All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.
 
           “All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.
 
       </p>
 
       </p>
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          </div>
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        <br /><br /><br /><br /><br /><br />
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        </div>
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           <p style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Plasmids used</p>
−
      </div>
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          <br />
−
      <div class="panel panel-transparent">
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        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
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        <div class="panel-heading">
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Kit Plate 7 <br />
−
          <h4 class="panel-title"><h3>Materials & Methods</h3></h4>
+
Positive Control (BBa_I20270): well 21B<br />
−
        </div>
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Negative Control (BBa_R0040): well 21D<br />
−
        <div id="Abstract" class="panel-collapse collapse in">
+
Test Device 1 (BBa_J364000): well 21F<br />
−
           <div class="panel-body">
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Test Device 2 (BBa_J364001): well 21H<br />
−
          <h4 style="border-bottom:1px solid black; padding-top:6px;padding-bottom: 12px">Plasmids used</h4>
+
Test Device 3 (BBa_J364002): well 21J<br />
−
            <p class="text-justify" style="text-align:center" >
+
Test Device 4 (BBa_J364003): well 21L<br />
−
Kit Plate 7 <br/>
+
Test Device 5 (BBa_J364004): well 21N<br />
−
 
+
Test Device 6 (BBa_J364005): well 21P<br />
−
Positive Control (BBa_I20270): well 21B<br/>
+
<br />
−
Negative Control (BBa_R0040): well 21D<br/>
+
−
Test Device 1 (BBa_J364000): well 21F<br/>
+
−
Test Device 2 (BBa_J364001): well 21H<br/>
+
−
Test Device 3 (BBa_J364002): well 21J<br/>
+
−
Test Device 4 (BBa_J364003): well 21L<br/>
+
−
Test Device 5 (BBa_J364004): well 21N<br/>
+
−
Test Device 6 (BBa_J364005): well 21P<br/>
+
−
<br/>
+
 
           </p>
 
           </p>
−
              <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Strain used</h4>
+
        <br /><br /><br />
−
            <p class="text-justify" style="text-align:center" >
+
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Strain used</p>
−
 
+
        <br />
−
Escherichia coli DH5α <br/>
+
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
−
 
+
        Escherichia coli DH5α<br />
−
 
+
        <br />
−
          </p>
+
        </p>
−
              <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Materials</h4>
+
        <br /><br /><br />
−
            <p class="text-justify" style="text-align:center" >
+
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Materials</p>
−
FITC Standard: one tube with dried down FITC for creating a FITC standard <br/>
+
        <br />
−
• LUDOX: one tube with 30% colloidal silica suspended in 1mL of water <br/>
+
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
−
• 1xPBS (phosphate buffered saline) <br/>
+
FITC Standard: one tube with dried down FITC for creating a FITC standard <br />
−
• LB (Luria Bertani) media <br/>
+
LUDOX: one tube with 30% colloidal silica suspended in 1mL of water <br />
−
• Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH) <br/>
+
1xPBS (phosphate buffered saline) <br />
−
• 50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth <br/>
+
LB (Luria Bertani) media <br />
−
• 1.5 ml eppendorf tubes for sample storage <br/>
+
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH) <br />
−
• Ice bucket with ice <br/>
+
50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth <br />
−
• Pipettes <br/>
+
1.5 ml eppendorf tubes for sample storage <br />
−
• 96 well plate  <br/>
+
Ice bucket with ice <br />
−
  <br/>
+
Pipettes <br />
−
 
+
96 well plate  <br />
−
 
+
  <br />
−
 
+
        </p>
−
 
+
        <br /><br /><br />
−
          </p>
+
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Machines</p>
−
<h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Machines</h4>
+
        <br />
−
            <p class="text-justify" style="text-align:center" >
+
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
−
 
+
        Enspire 2300-001M<br />
−
Enspire 2300-001M<br/>
+
        Sigma Laborzentrifugen<br />
−
Sigma Laborzentrifugen<br/>
+
        Thermo Scientific Nanodrop 2000<br />
−
Thermo Scientific Nanodrop 2000<br/>
+
        EVOS AMG microscope<br />
−
EVOS AMG microscope<br/>
+
        <br/>
−
 
+
        </p>
−
 
+
        <br /><br /><br />
−
          </p>
+
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Software</p>
−
              <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Software</h4>
+
        <br />
−
            <p class="text-justify" style="text-align:center" >
+
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
−
 
+
        Microsoft Excel<br />
−
Microsoft Excel<br/>
+
        <br/>
−
 
+
        </p>
−
 
+
        <br /><br /><br />
−
          </p>
+
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Methods</p>
−
 
+
        <br />
−
 
+
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
−
 
+
        Calibration<br />
−
 
+
        OD600 Reference point<br />
−
 
+
        FITC fluorescence standard curve<br />
−
 
+
        Test Device 1 (BBa_J364000): well 21F<br />
−
              <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Methods</h4>
+
        Test Device 2 (BBa_J364001): well 21H<br />
−
<p class="text-justify" style="text-align:center" >
+
        Test Device 3 (BBa_J364002): well 21J<br />
−
 
+
        Test Device 4 (BBa_J364003): well 21L<br />
−
Calibration<br/>
+
        Test Device 5 (BBa_J364004): well 21N<br />
−
OD600 Reference point<br/>
+
        Test Device 6 (BBa_J364005): well 21P<br />
−
FITC fluorescence standard curve<br/>
+
        <br/>
−
Test Device 1 (BBa_J364000): well 21F<br/>
+
        </p>
−
Test Device 2 (BBa_J364001): well 21H<br/>
+
        <br /><br /><br />
−
Test Device 3 (BBa_J364002): well 21J<br/>
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        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Cell measurement</p>
−
Test Device 4 (BBa_J364003): well 21L<br/>
+
        <br />
−
Test Device 5 (BBa_J364004): well 21N<br/>
+
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial">
−
Test Device 6 (BBa_J364005): well 21P<br/>
+
        Transformation<br />
−
 
+
        Measurements<br />
−
          </p>
+
        <br/>
−
 
+
        </p>
−
              <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Cell measurement</h4>
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        <br /><br /><br />
−
            <p class="text-justify" style="text-align:center" >
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        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Protocols</p>
−
 
+
        <br />
−
Transformation<br/>
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        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
−
Measurements
+
        <a href="http://parts.igem.org/Help:Protocols/Transformation" style="color:blue">Please click here</a>
−
<br/>
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        <br/>
−
 
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        </p>
−
 
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        <br /><br /><br /><br /><br /><br />
−
          </p>
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        <object width="70%" height="800px" data="https://static.igem.org/mediawiki/2017/e/e8/Wiki_of_InterLab_by_UST_Beijing.pdf">  
−
 
+
−
 
+
−
 
+
−
<h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Protocols</h4>
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−
            <p class="text-justify" style="text-align:center" >
+
−
 
+
−
Please click the link:<br/>
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−
<a href="https://2017.igem.org/Competition/InterLab_Study">https://2017.igem.org/Competition/InterLab_Study</a>>
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−
<br/><br/><br/><br/><br/>
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−
 
+
−
 
+
−
          </p>
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−
<div class="panel-heading" role="tab">
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−
          <h4 class="panel-title"><h3>Result</h3></h4>
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−
        </div>
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−
<br/><br/>
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−
              <object width="100%" height="500" data="https://static.igem.org/mediawiki/2017/e/e8/Wiki_of_InterLab_by_UST_Beijing.pdf">
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         </object>
 
         </object>
−
          </div>
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</div>
−
        </div>
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  <br /><br /><br /><br />
−
      </div>
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−
  </div>
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</div>
 
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</div>
 
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</section>
 
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<section class="page_section team" id="team" style="background-image:url(https://static.igem.org/mediawiki/2017/f/f8/Ustbbjbj.png);background-position:center center;background-repeat:no-repeat;-moz-background-size: cover;background-size: cover;background-attachment:fixed;">
 
−
  <div class="container">
 
−
    <h2 style="font-family: Arial">Sponsorship</h2>
 
−
    <br />
 
−
<div id="team" name="team">
 
−
  <div class="container">
 
−
    <div class="row centered">
 
−
 
−
      <div class="col-md-3 centered"> <img class="img img-circle lt-box" src="https://static.igem.org/mediawiki/2017/d/da/T--UST_Beijing--xteam01.jpg" height="120px" width="120px" alt="">
 
−
        <div class="rt-box"><h4><strong>USTB</strong></h4>
 
−
        <p style="text-align: center">University of Science & Technology Beijing</p><br/>
 
−
</div>
 
−
      </div>
 
−
        <div class="col-md-3 centered"> <img class="img img-circle lt-box" src="https://static.igem.org/mediawiki/2017/3/30/USTB_huasheng_logo.png" height="120px" width="120px" alt="">
 
−
        <div class="rt-box"><h4><strong>USTB-CBE</strong></h4>
 
−
        <p style="text-align: center">University of Science & Technology Beijing<br />School of Chemistry and Biological Engineering</p><br/>
 
−
</div>
 
−
      </div>
 
−
      <div class="col-md-3 centered"> <img class="img img-circle lt-box" src="https://static.igem.org/mediawiki/2017/9/95/T--UST_Beijing--xteam03.jpg" height="120px" width="120px" alt="">
 
−
        <div class="rt-box"><h4><strong>BCU</strong></h4>
 
−
        <p style="text-align: center">Beijing City University</p><br/>
 
−
</div>
 
−
      </div>
 
−
      <div class="col-md-3 centered"> <img class="img img-circle lt-box" src="https://static.igem.org/mediawiki/2017/8/81/T--UST_Beijing--xteam02.jpg" height="120px" width="120px" alt="">
 
−
        <div class="rt-box"><h4><strong>YUNNAN NOTOGINSENG</strong></h4>
 
−
        <p style="text-align: center">An enterprise sponsor.</p><br/>
 
−
</div>
 
−
      </div>
 
−
 
−
    </div>
 
−
  </div>
 
−
</div>
 
 
     <div class="container" align="center">
 
     <div class="container" align="center">
 
         <p align="center" style="font-size: 5px"><br><h5>Copyright © 2017 UST_Beijing iGEM. All rights reserved.</h5></p>
 
         <p align="center" style="font-size: 5px"><br><h5>Copyright © 2017 UST_Beijing iGEM. All rights reserved.</h5></p>
 
     </div>
 
     </div>
−
</div>
+
   
 
</section>
 
</section>
 +
 
</body>
 
</body>
  

Latest revision as of 16:59, 1 November 2017

USTB-Beijing | Welcome




InterLab Measurement Study


“All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.







Plasmids used


Kit Plate 7
Positive Control (BBa_I20270): well 21B
Negative Control (BBa_R0040): well 21D
Test Device 1 (BBa_J364000): well 21F
Test Device 2 (BBa_J364001): well 21H
Test Device 3 (BBa_J364002): well 21J
Test Device 4 (BBa_J364003): well 21L
Test Device 5 (BBa_J364004): well 21N
Test Device 6 (BBa_J364005): well 21P




Strain used


Escherichia coli DH5α




Materials


FITC Standard: one tube with dried down FITC for creating a FITC standard
LUDOX: one tube with 30% colloidal silica suspended in 1mL of water
1xPBS (phosphate buffered saline)
LB (Luria Bertani) media
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH)
50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth
1.5 ml eppendorf tubes for sample storage
Ice bucket with ice
Pipettes
96 well plate




Machines


Enspire 2300-001M
Sigma Laborzentrifugen
Thermo Scientific Nanodrop 2000
EVOS AMG microscope




Software


Microsoft Excel




Methods


Calibration
OD600 Reference point
FITC fluorescence standard curve
Test Device 1 (BBa_J364000): well 21F
Test Device 2 (BBa_J364001): well 21H
Test Device 3 (BBa_J364002): well 21J
Test Device 4 (BBa_J364003): well 21L
Test Device 5 (BBa_J364004): well 21N
Test Device 6 (BBa_J364005): well 21P




Cell measurement


Transformation
Measurements




Protocols


Please click here












Copyright © 2017 UST_Beijing iGEM. All rights reserved.