Difference between revisions of "Team:BostonU/Experiments"

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In order to characterize the fluorescence capabilities of our cell-free system, we measured the fluorescence from a plasmid coding for a  constitutively active deGFP at varying concentrations. Our plasmid of choice was pBEST, which comes from the Noireaux Lab, whose cell-free protocol we used to make our in house cell-free. pBEST was shown by the Noireaux lab to have high performance in cell-free [1]. The plasmid was designed modularly, so each part could be replaced using a simple digestion ligation reaction. </p>
 
In order to characterize the fluorescence capabilities of our cell-free system, we measured the fluorescence from a plasmid coding for a  constitutively active deGFP at varying concentrations. Our plasmid of choice was pBEST, which comes from the Noireaux Lab, whose cell-free protocol we used to make our in house cell-free. pBEST was shown by the Noireaux lab to have high performance in cell-free [1]. The plasmid was designed modularly, so each part could be replaced using a simple digestion ligation reaction. </p>
 
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Tthe plasmid was at added to cell-free at 0 nM, 10 nM, 20 nM, 30 nM, and 40 nM concentrations. Fluorescence was measured over eight hours. The data was then used to inform our <a href="https://2017.igem.org/Team:BostonU/Model">model</a>.</p>
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The plasmid was added to cell-free at 0 nM, 10 nM, 20 nM, 30 nM, and 40 nM concentrations. Fluorescence was measured over eight hours. The data was then used to inform our <a href="https://2017.igem.org/Team:BostonU/Model">model</a>.</p>
 
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Revision as of 17:27, 1 November 2017

EXPERIMENTS