Difference between revisions of "Team:Lethbridge HS/Composite Part"

 
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background-image: url("https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--TeamCover4.jpeg");text-align:center;  
 
background-image: url("https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--TeamCover4.jpeg");text-align:center;  
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     background-size: cover; background-position: center; opacity: ; background-size:  ; height: 50vh;
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<body id="background">
 
<body id="background">
 
<div id="banner" class=" jumbotron text-center "><div class="container-fluid">
 
<div id="banner" class=" jumbotron text-center "><div class="container-fluid">
−
     <h1 style=" font-size: 100px;">Composite Part</h1>
+
     <h1 style=" font-size: 100px;">Composite Parts</h1>
 
   </div>  
 
   </div>  
 
   </div>
 
   </div>
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<br>
 
<br>
 
<br>
 
<br>
−
<br>
+
<br>  
−
<h2>&nbsp;Anthocyanin Construct</h2>
+
<div class="container-fluid" style="margin-left:10%; margin-right:10%;">
−
<p class="center"> This construct is being used to convert our initial molecule Eriodictyol into our final molecule Anthocyanin. The genes are:
+
<h2><b>&nbsp;Melanin Construct</b></h2>
 +
<h3>Melanin composite part</h3>
 +
<p class="center">
 +
<a href="http://parts.igem.org/Part:BBa_K2481108">BBa_K2481108</a>
 +
<br>
 +
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 +
<br>
 +
<b><i>mel</i>A</b> Part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K193600">BBa_K193600</a>.
 +
<br>
 +
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
<br>
 +
<br>
 +
This construct allows us to make Melanin out of L-Tyrosine.
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/a/ad/T--Lethbridge_HS--MelaninPathway.png"></center>
 +
</div>
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--MelaninConstruct2.png"></center>
 +
<p> <i>Figure 1. melA expression construct for use in <i>E. coli</i> BL21(DE3).</i></p>
 +
</div>
 +
 
 +
<h2><b>&nbsp;Anthocyanin Constructs</b></h2>
 +
<br>
 +
<h3>Anthocyanin composite part 1</h3>
 +
<p class="center">
 +
<a href="http://parts.igem.org/Part:BBa_K2481113">BBa_K2481113</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
+
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481110">BBa_K2481110</a>
 
<br>
 
<br>
−
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
+
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481111">BBa_K2481111</a>
 
<br>
 
<br>
−
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
+
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 
<br>
 
<br>
−
<b><i>3gt</i></b> Part BBa_waitingforsubmission
+
 
 +
<h3>Anthocyanin composite part 2</h3>
 +
<p class="center">
 +
<a href="http://parts.igem.org/Part:BBa_K2481114">BBa_K2481114</a>
 
<br>
 
<br>
−
<b><i>yad</i>H</b> Part BBa_alsowaiting
+
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 +
<br>
 +
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481112">BBa_K2481112</a>
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 
<br>
 
<br>
−
These genes will all be used in the cell to produce Anthocyanin out of Eriodictyol. The <i>yad</i>H has been shown to increase yields even though it is not directly involved in the pathway. The <i>3gt</i> as well as <i>yad</i> H are two of our original basic part submissions. This Construct is our composite part submission BBa_anthocyanincompsitepart.
+
 
 +
<h3>Anthocyanin composite part 3</h3>
 +
<p class="center">
 +
<a href="http://parts.igem.org/Part:BBa_K2481105">BBa_K2481105</a>
 +
<br>
 +
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 +
<br>
 +
<b><i>3gt</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481002">BBa_K2481002</a>
 +
<br>
 +
<b><i>yad</i>H</b> Part <a href="http://parts.igem.org/Part:BBa_K2481004">BBa_K2481004</a>
 +
<br>
 +
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
<br>
 +
<br>
 +
These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted
 
<br>
 
<br>
  
  
−
<div style="text-align:center;"><center><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/1/15/T--Lethbridge_HS--AnthocyaninPathway.png" height="150"><center></div>
+
<div style="text-align:center;"><center><img height=50px class="img-responsive"src="https://static.igem.org/mediawiki/2017/1/15/T--Lethbridge_HS--AnthocyaninPathway.png"></center></div>
 
<br>
 
<br>
−
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/24/T--Lethbridge_HS--AnthocyaninConstruct.png"></center></div>
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/d/d2/T--Lethbridge_HS--AnthocyaninConstruct2.png"></center></div>
 
<br>
 
<br>
−
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/b/b4/T--Lethbridge_HS--yadHconstruct.png"></center>
+
<div style="text-align:center;"></center>
 +
</div>
 +
<div style="text-align:center;">
 
</div>
 
</div>
 
</p>
 
</p>
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<br>
 
<br>
  
−
<h2>&nbsp;Zeaxanthin Construct</h2>
+
<h2><b>&nbsp;Zeaxanthin Construct</b></h2>
−
<p class="center"> This construct is made up of the following genes:
+
<h3>Zeaxanthin composite part</h3>
 +
<p class="center">
 +
<a href="http://parts.igem.org/Part:BBa_K2481107">BBa_K2481107</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 113: Line 170:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>crt</i>Y</b> Part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I742154">BBa_I742154</a>.
+
<b><i>crtY</i></b> Part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I742154">BBa_I742154</a>.
 
<br>
 
<br>
−
<b><i>crt</i>Z</b> Part <a href"http://parts.igem.org/Part:BBa_I742157">BBa_I742157</a>.
+
<b><i>crtZ</i></b> Part <a href="http://parts.igem.org/Part:BBa_I742157">BBa_I742157</a>.
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
These parts all come together in our composite part <!-- link to the part--> BBa_Zeaxanthinconstruct. This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.
+
<br>
 +
This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.
 
<br>
 
<br>
 
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/8/8f/T--Lethbridge_HS--ZeaxanthinPathway.png"></center>
 
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/8/8f/T--Lethbridge_HS--ZeaxanthinPathway.png"></center>
 
</div>
 
</div>
 
<br>
 
<br>
−
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/c/c5/T--Lethbridge_HS--ZeaxanthinConstruct.png"></center>
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/e/eb/T--Lethbridge_HS--ZeaxanthinConstruct2.png"></center>
 
</div>
 
</div>
 
</p>
 
</p>
−
<br>
 
 
<br>
 
<br>
  
−
<h2>&nbsp;Indigoidine Construct</h2>
+
<h2><b>&nbsp;Indigoidine Constructs</b></h2>
−
<p class="center">Our Indigoidine construct is made up of:
+
<h3>Indigoidine composite part 1</h3>
 +
<p class="center">
 +
<a href="http://parts.igem.org/Part:BBa_K2481106">BBa_K2481106</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 137: Line 196:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>ind</i>B</b> Part <!--waiting for our part submission here-->BBa_waiting for actual part submission.
+
<b><i>ind</i>B</b> Part <a href="http://parts.igem.org/Part:BBa_K2481001">BBa_K2481001</a>
−
<br>
+
−
<b><i>ind</i>C</b> Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>
+
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
−
<br>
+
</p>
−
These two genes come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is one of our original basic part submissions. This construct is part BBa_idkyet
+
−
<br>
+
−
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center>
+
−
</div>
+
−
<br>
+
−
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/25/T--Lethbridge_HS--IndigoidineConstruct.png"></center>
+
−
</div>
+
−
</p>
+
−
<br>
+
 
<br>
 
<br>
  
−
 
+
<h3>Indigoidine composite part 2</h3>
−
<h2>&nbsp;Melanin Construct</h2>
+
<p class="center">
−
<p class="center">The parts used in our Melanin construct are as follows:
+
<a href="http://parts.igem.org/Part:BBa_K2481109">BBa_K2481109</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 162: Line 210:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>Mel</i>A</b> Part <a href="http://parts.igem.org/Part:BBa_K274001">BBA_K274001</a>.
+
<b><i>ind</i>C</b> Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
This construct allows us to make Melanin out of L-Tyrosine. it is Part BBa_waitandsee
 
 
<br>
 
<br>
−
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/a/ad/T--Lethbridge_HS--MelaninPathway.png"></center>
+
These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. We had to split our genes into two separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center>
 
</div>
 
</div>
 
<br>
 
<br>
−
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--MelaninConstruct.png"></center>
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/a/a3/T--Lethbridge_HS--IndigoidineConstruct2.png"></center>
 
</div>
 
</div>
 +
</p>
 +
<br>
 +
<br>
 +
 +
 +
 
</p>
 
</p>
 
<br>
 
<br>
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</body>
 
</body>
  
 
</html>
 
</html>

Latest revision as of 17:41, 1 November 2017





 Melanin Construct

Melanin composite part

BBa_K2481108
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
melA Part BBa_K193600.
Terminator Part BBa_B0015.

This construct allows us to make Melanin out of L-Tyrosine.


Figure 1. melA expression construct for use in E. coli BL21(DE3).

 Anthocyanin Constructs


Anthocyanin composite part 1

BBa_K2481113
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
dfr Part BBa_K2481110
f3h Part BBa_K2481111
Terminator Part BBa_B0015.


Anthocyanin composite part 2

BBa_K2481114
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
ans Part BBa_K2481112
Terminator Part BBa_B0015.


Anthocyanin composite part 3

BBa_K2481105
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
3gt Part BBa_K2481002
yadH Part BBa_K2481004
Terminator Part BBa_B0015.

These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted





 Zeaxanthin Construct

Zeaxanthin composite part

BBa_K2481107
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.

This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.



 Indigoidine Constructs

Indigoidine composite part 1

BBa_K2481106
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_K2481001
Terminator Part BBa_B0015.


Indigoidine composite part 2

BBa_K2481109
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indC Part BBa_K1152013
Terminator Part BBa_B0015.

These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. We had to split our genes into two separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.