Difference between revisions of "Team:UCLouvain/Basic Part"

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<h3>★  ALERT! </h3>
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<div class="page-title">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<div class="big-title montserrat-text uppercase">Parts</div>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="small-title montserrat-text uppercase"><br><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">BactaSun</Marine></div>
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<div class="container">
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<div class="row" style="margin-bottom: 40px;">
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<div class="col-md-6">
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      <div class="section-title" style="text-align:center;float:left;width:100%;margin-bottom:0">
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<span>Auxotrophic Approach</span>
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      </div>
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<BR><BR>
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      <div class="section-title" style="text-align:center;float:left;width:100%;margin-bottom:0">
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<span><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">Basic parts</Marine></span>
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<BR><BR>
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      <div class="section-title" style="text-align:center;float:left;width:100%;margin-bottom:0">
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<p><i><center>Source : iGEM DNA Distribution Kit</center></i></p>
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      </div>
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<BR><BR>
  
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                        <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<p class="montserrat-text uppercase">pBAD/araC <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_I0500">(I0500)</a></p>
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                        <p>The pBAD promoter is a very common inducible one that is activated by L-arabinose and repressed by araC.</p>
  
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<BR><BR>
  
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<h1>Basic Parts</h1>
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  <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<p class="montserrat-text uppercase">Terminator <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_B0010">(B0010)</a></p>
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<p>
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                            <p>Terminators are necessary to stop the transcription at the end of a coding sequence.</p>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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</p>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<BR><BR>
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<h3>Best Basic Part Special Prize</h3>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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  <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<p class="montserrat-text uppercase">RBS <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_B0034">(B0034)</a></p>
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  </div>
  
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                            <p>Efficient ribosome binding site characterized by IIT Madras in iGEM 2016.</p>
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<BR><BR>
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    <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<p class="montserrat-text uppercase">RFP <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_E1010">(E1010)</a></p>
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    </div>
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                            <p>Fluorescent proteins are very common reporter genes. This particular one comes from <i>Discosoma striata</i> and was engineered to be monomeric. The excitation peak is at 584 nm, and the emission peak at 607 nm.</p>
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    <BR><BR>
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      <div class="section-title" style="text-align:center;float:left;width:100%;margin-bottom:0">
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<span><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">Composite parts</Marine></span>
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      </div>
 
<br><br>
 
<br><br>
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<br>
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<p class="montserrat-text uppercase">piFinal <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_K577882">(BBa_K577882)</a></p>
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</div>
  
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                        <p>iGEM plasmid consisting of araC, a pBAD/AraC promoter (I0500), an RBS (B0034), an RFP (E1010), and two terminators (B0010 and B0012) contained inside one biobrick (B0015)</p>
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<img src="https://static.igem.org/mediawiki/2017/3/34/UCLOUVAIN_-_BBa_K577882_approach_1.png" class="in_text_img" style="width: 500px;">
  
  
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<div class="highlight">
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                        </div>
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<h4>Note</h4>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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                        <div class="col-md-6">
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    <div class="section-title" style="text-align:center;float:left;width:100%;margin-bottom:0">
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<span>COMR/S Approach</span>
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<BR><BR>
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      <div class="section-title" style="text-align:center;float:left;width:100%;margin-bottom:0">
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<span><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">Basic parts</Marine></span>
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      </div>
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<BR><BR>
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      <div class="section-title" style="text-align:center;float:left;width:100%;margin-bottom:0">
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<p><center><i>New parts</i></center></p>
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      </div>
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<BR><BR>
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                            <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<p class="montserrat-text uppercase">ComR <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_K2341000">(BBa_K2341000)</a></p>
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    </div>
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                        <p>Protein originally found in <i>Streptococcus thermophilius</i> where it regulates natural competence. The protein activation and binding to specific promoters (ComR Box; BBa_K2341001) is only possible in presence of a short peptide, ComS (LPYFAGCL). Binding to the promoter will induce the downstream sequence expression.</p>
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<BR><BR>
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    <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<p class="montserrat-text uppercase">P1655 promoter <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_K2341000">(BBa_K2341001)</a></p>
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    </div>
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                        <p>Promote expression of the sequence downstream in presence of ComR (BBa_K2341000) and ComS (LPYFAGCL). Sequence containing the palindrome motif needed to bind ComR (= ComR box).</p>
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<BR><BR>
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    <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<p class="montserrat-text uppercase">sgRNA Tyrosin (Cas9 guide) <a style="color:#0066cc;" href="http://parts.igem.org/Part:BBa_K2341100">(BBa_K2341100)</a></p>
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    </div>
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                        <p>RNA guide to use in combination with CRISPR/CAS9. Cut inside the tyrA gene so that deletion by homologous recombination can occur if a homologous DNA strand is also added. RNA guide under constitutive expression by BBa_J23119.</p>
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<BR><BR>
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      <div class="section-title" style="text-align:center;float:left;width:100%;margin-bottom:0">
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<span><Marine style="margin-left:32px;font-family:'Brush Script MT';font-size:32px;text-transform:none;font-weight:bold;">Composite parts</Marine></span>
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      </div>
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<br><br>
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              <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0">
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<p class="montserrat-text uppercase">pCMMCD 105</p>
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</div>
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                        <p>Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.</p>
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                        <p>GusA encodes the <i>beta</i>-glucuronidase gene from <i>E. coli</i>. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and <i>para</i>-nitrophenyl glucuronide.</p>
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<p>Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).</p>
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<BR><BR>
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<br><br>
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</div>
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<BR><br>
 
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<center><groupparts>iGEM17 UCLouvain</groupparts></center>
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{{UCLouvainFooter}}

Latest revision as of 17:45, 1 November 2017

iGEM UCLouvain Team iGEM UCLouvain Team

Parts

BactaSun
Auxotrophic Approach


Basic parts


Source : iGEM DNA Distribution Kit



pBAD/araC (I0500)

The pBAD promoter is a very common inducible one that is activated by L-arabinose and repressed by araC.



Terminator (B0010)

Terminators are necessary to stop the transcription at the end of a coding sequence.



RBS (B0034)

Efficient ribosome binding site characterized by IIT Madras in iGEM 2016.



RFP (E1010)

Fluorescent proteins are very common reporter genes. This particular one comes from Discosoma striata and was engineered to be monomeric. The excitation peak is at 584 nm, and the emission peak at 607 nm.



Composite parts


piFinal (BBa_K577882)

iGEM plasmid consisting of araC, a pBAD/AraC promoter (I0500), an RBS (B0034), an RFP (E1010), and two terminators (B0010 and B0012) contained inside one biobrick (B0015)

COMR/S Approach

Basic parts


New parts



ComR (BBa_K2341000)

Protein originally found in Streptococcus thermophilius where it regulates natural competence. The protein activation and binding to specific promoters (ComR Box; BBa_K2341001) is only possible in presence of a short peptide, ComS (LPYFAGCL). Binding to the promoter will induce the downstream sequence expression.



P1655 promoter (BBa_K2341001)

Promote expression of the sequence downstream in presence of ComR (BBa_K2341000) and ComS (LPYFAGCL). Sequence containing the palindrome motif needed to bind ComR (= ComR box).



sgRNA Tyrosin (Cas9 guide) (BBa_K2341100)

RNA guide to use in combination with CRISPR/CAS9. Cut inside the tyrA gene so that deletion by homologous recombination can occur if a homologous DNA strand is also added. RNA guide under constitutive expression by BBa_J23119.



Composite parts


pCMMCD 105

Plasmid consisting of ComR (BBa_K2341000), the p1655 promoter (containing the ComR box, BBa_K2341001), GusA and an erythromycin resistance.

GusA encodes the beta-glucuronidase gene from E. coli. This is a popular reporter gene that uses glucuronides as substrates, the most common of which are X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) and para-nitrophenyl glucuronide.

Here, it is under the control of the ComRS pathway and uses the ComS peptide from S. thermophilius (LPYFAGGL).







<groupparts>iGEM17 UCLouvain</groupparts>

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