Difference between revisions of "Team:Oxford/Protein Based Experiments"

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<h1> Future Protein-Based System Experiments <h1/>
 
<h1> Future Protein-Based System Experiments <h1/>
 
<h2> Introduction <h2/>
 
<h2> Introduction <h2/>

Revision as of 18:22, 1 November 2017

Future Protein-Based System Experiments

Introduction

In our project, the central sfGFP-dark quencher part has been shown to have repressed fluorescence which is relieved on cleavage by TEV, and this fluorescence is unaffected by the presence of the TorA leader sequence, spycatcher and His tag. This represents the first design built test cycle, proving the concept of relieved repression at this point, and as will be seen, this will be used throughout our suggestions for future experiments. We have also designed the parts that would be used to target proteins to the membranes, and will explain the future experiments to test these.