Difference between revisions of "Team:Michigan/Improve"

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{{Michigan}}
 
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<title>Team Michigan: Contributions</title>
  
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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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<h2>Improve</h2>
  
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*First of all we must clarify a clerical mistake made on the judging form. We misread the
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instructions and put the link of our new parts rather than the part we improved the characterization for which is part  BBa_K112805 to fulfill gold medal requirement #2. </b>
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Due to the fact that we could not find literature on the kinetics of interaction between these proteins we decided to attempt to characterize this aspect in our project. Since we decided on integrating holin and endolysin in our system, as outlined in our <a target="_blank" href="https://2017.igem.org/Team:Michigan/Design"> PROJECT DESIGN</a> page, we looked to part <a target="_blank" href="http://parts.igem.org/Part:BBa_K112805"> BBa_K112805</a> that is part of the registry but had some major flaws. This is our improvement:
  
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<strong><a target="_blank" href="http://parts.igem.org/Part:BBa_K2301000"> BBa_K2301000</a> T4 Holin:</strong>
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We improved the function of part <a target="_blank" href="http://parts.igem.org/Part:BBa_K112805"> BBa_K112805</a> from UC Berkeley’s 2008 project by optimizing the codons for translation in E. coli using IDT’s codon optimization tool and by eliminating the illegal XbaI site that Imperial College London’s 2011 team found, making the part much easier for future iGEM teams to use. The changes we made were T358C, T556C, T563C, and T571C. We then had the gene synthesized by IDT and integrated it into our construct as outlined in our <a target="_blank" href="https://2017.igem.org/Team:Michigan/Experiments"> EXPERIMENTS</a> page.
  
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<li onClick="go('')"><a class="subtitle">thermolyze</a></li>
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<li onClick="go('/Safety')"><a class="subtitle">safety</a></li>
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<li onClick="go('/Attributions')"><a class="subtitle">attributions</a></li>
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Revision as of 18:46, 1 November 2017

Team Michigan: Contributions

Improve


*First of all we must clarify a clerical mistake made on the judging form. We misread the instructions and put the link of our new parts rather than the part we improved the characterization for which is part BBa_K112805 to fulfill gold medal requirement #2.
Due to the fact that we could not find literature on the kinetics of interaction between these proteins we decided to attempt to characterize this aspect in our project. Since we decided on integrating holin and endolysin in our system, as outlined in our PROJECT DESIGN page, we looked to part BBa_K112805 that is part of the registry but had some major flaws. This is our improvement: BBa_K2301000 T4 Holin: We improved the function of part BBa_K112805 from UC Berkeley’s 2008 project by optimizing the codons for translation in E. coli using IDT’s codon optimization tool and by eliminating the illegal XbaI site that Imperial College London’s 2011 team found, making the part much easier for future iGEM teams to use. The changes we made were T358C, T556C, T563C, and T571C. We then had the gene synthesized by IDT and integrated it into our construct as outlined in our EXPERIMENTS page.