Difference between revisions of "Team:ASTWS-China"

 
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        <a href="https://2017.igem.org/Team:ASTWS-China">HOME</a>
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        <a class="sub-nav-toggle">TEAM</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Members">Members</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Attributions">Attributions</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Collaborations">Collaborations</a>
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        <a class="sub-nav-toggle">PROJECT</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Description">Description</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Design">Design</a>
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        <a class="sub-nav-toggle">Experiment</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/PROTOCOLS">PROTOCOLS</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Results">Results</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Model">Model</a>
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        <a class="sub-nav-toggle">PARTS</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Basic_Part" >Basic Parts</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Composite_Part">Composite Parts</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Part_Collection">Part Collection</a>
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        <a class="sub-nav-toggle">HUMAN PRACTICE</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/HP/Silver" class="selected">Silver</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/HP/Gold_Integrated">Integrated and Gold</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Engagement">EDUCATION & PUBLIC ENGAGEMENT</a>
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        <a href="https://2017.igem.org/Team:ASTWS-China/InterLab">InterLab</a>
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<h1> Welcome to iGEM 2017! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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          <h1 id="logo">
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            <a href="https://2017.igem.org/Team:ASTWS-China">ASTWS-China</a>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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          </h1>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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                    <a href="https://2017.igem.org/Team:ASTWS-China/Members">
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                      <img src="https://static.igem.org/mediawiki/2017/f/fc/T-ASTWS-China-f1.png" alt=""/>
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                    <a href="https://2017.igem.org/Team:ASTWS-China/Design">
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                      <img src="https://static.igem.org/mediawiki/2017/5/57/T-ASTWS-China-f2.png" alt=""/>
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                    <a href="https://2017.igem.org/Team:ASTWS-China/Results">
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                      <img src="https://static.igem.org/mediawiki/2017/6/61/T-ASTWS-China-f3.png" alt=""/>
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                    <a href="https://2017.igem.org/Team:ASTWS-China/HP/Silver">
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                      <img src="https://static.igem.org/mediawiki/2017/7/7b/T-ASTWS-China-f4.png" alt=""/>
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                    <a href="https://2017.igem.org/Team:ASTWS-China/Basic_Part">
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                      <img src="https://static.igem.org/mediawiki/2017/d/d1/T-ASTWS-China-f5.png" alt=""/>
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                    <a href="https://2017.igem.org/Team:ASTWS-China/InterLab">
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                      <img src="https://static.igem.org/mediawiki/2017/2/2e/T-ASTWS-China-f6.png" alt=""/>
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              <h2 class="header">Smart E.coli for B Cells Capture</h2>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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                Antibodies are Y-shaped proteins secreted by B lymphocytes that specifically recognize and neutralize antigens. Since the first monoclonal antibody was produced by hybridoma technology in 1975, it has become an important tool in both research and health care.
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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                Here we developed a smart E.coli system for capturing B lymphocytes by utilizing the high affinity interaction between antigen and antibody. A peptide fused with RSA and GFP is expressed and purified as antigens for immunization and Elisa assay respectively, and this target peptide and an affinity tag are co-expressed on the surface of E.coli. Coupled with anti-tag magnetic beads, it can be used to screen and capture the desired B cells that secret the corresponding antibodies. These B cells can be fused or gene cloned for antibody producing. This system can also be applied to many aspects of enormous value, such as vaccine production and immune T-cell or tumor cell separation.
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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Latest revision as of 19:31, 1 November 2017

Smart E.coli for B Cells Capture

Antibodies are Y-shaped proteins secreted by B lymphocytes that specifically recognize and neutralize antigens. Since the first monoclonal antibody was produced by hybridoma technology in 1975, it has become an important tool in both research and health care.

Here we developed a smart E.coli system for capturing B lymphocytes by utilizing the high affinity interaction between antigen and antibody. A peptide fused with RSA and GFP is expressed and purified as antigens for immunization and Elisa assay respectively, and this target peptide and an affinity tag are co-expressed on the surface of E.coli. Coupled with anti-tag magnetic beads, it can be used to screen and capture the desired B cells that secret the corresponding antibodies. These B cells can be fused or gene cloned for antibody producing. This system can also be applied to many aspects of enormous value, such as vaccine production and immune T-cell or tumor cell separation.

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