Difference between revisions of "Team:ASTWS-China"

 
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    <ul id="options" class="clearfix">
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      <li>
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        <a href="https://2017.igem.org/Team:ASTWS-China">HOME</a>
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        <a class="sub-nav-toggle">TEAM</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Members">Members</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Attributions">Attributions</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Collaborations">Collaborations</a>
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        <a class="sub-nav-toggle">PROJECT</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Description">Description</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Design">Design</a>
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        </ul>
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        <a class="sub-nav-toggle">Experiment</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/PROTOCOLS">PROTOCOLS</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Results">Results</a>
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          </li>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Model">Model</a>
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        <a class="sub-nav-toggle">PARTS</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Basic_Part" >Basic Parts</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Composite_Part">Composite Parts</a>
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          </li>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Part_Collection">Part Collection</a>
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        </ul>
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      <li>
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        <a class="sub-nav-toggle">HUMAN PRACTICE</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/HP/Silver" class="selected">Silver</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China/HP/Gold_Integrated">Integrated and Gold</a>
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          </li>
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            <a href="https://2017.igem.org/Team:ASTWS-China/Engagement">EDUCATION & PUBLIC ENGAGEMENT</a>
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        </ul>
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        <a href="https://2017.igem.org/Team:ASTWS-China/InterLab">InterLab</a>
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            <a href="https://2017.igem.org/Team:ASTWS-China">ASTWS-China</a>
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<a class="page-scroll" href="#about">About</a>
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                    <a href="https://2017.igem.org/Team:ASTWS-China/Basic_Part">
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                      <img src="https://static.igem.org/mediawiki/2017/d/d1/T-ASTWS-China-f5.png" alt=""/>
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<a class="page-scroll" href="#team">Team</a>
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              <h2 class="header">Smart E.coli for B Cells Capture</h2>
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                Antibodies are Y-shaped proteins secreted by B lymphocytes that specifically recognize and neutralize antigens. Since the first monoclonal antibody was produced by hybridoma technology in 1975, it has become an important tool in both research and health care.
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                Here we developed a smart E.coli system for capturing B lymphocytes by utilizing the high affinity interaction between antigen and antibody. A peptide fused with RSA and GFP is expressed and purified as antigens for immunization and Elisa assay respectively, and this target peptide and an affinity tag are co-expressed on the surface of E.coli. Coupled with anti-tag magnetic beads, it can be used to screen and capture the desired B cells that secret the corresponding antibodies. These B cells can be fused or gene cloned for antibody producing. This system can also be applied to many aspects of enormous value, such as vaccine production and immune T-cell or tumor cell separation.
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A creative agency based on Candy Land, ready to boost your business with some beautifull templates. Lattes Agency is one of the best in town see more you will be amazed.
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Our portfolio is the best way to show our work, you can see here a big range of our work. Check them all and you will find what you are looking for.
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Latest revision as of 19:31, 1 November 2017

Smart E.coli for B Cells Capture

Antibodies are Y-shaped proteins secreted by B lymphocytes that specifically recognize and neutralize antigens. Since the first monoclonal antibody was produced by hybridoma technology in 1975, it has become an important tool in both research and health care.

Here we developed a smart E.coli system for capturing B lymphocytes by utilizing the high affinity interaction between antigen and antibody. A peptide fused with RSA and GFP is expressed and purified as antigens for immunization and Elisa assay respectively, and this target peptide and an affinity tag are co-expressed on the surface of E.coli. Coupled with anti-tag magnetic beads, it can be used to screen and capture the desired B cells that secret the corresponding antibodies. These B cells can be fused or gene cloned for antibody producing. This system can also be applied to many aspects of enormous value, such as vaccine production and immune T-cell or tumor cell separation.

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