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               <h2 class="header">Smart E.coli for B Cells Capture</h2>
 
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                 Our idea of project comes from Hybridoma technology. We found some disadvantage about the old technology. In order to improve this technology, we started this project. We also want to know how most of people thinks about Hybridoma technology and our new approach. So we make a questioner to collect others view about our project.
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                 Antibodies are Y-shaped proteins secreted by B lymphocytes that specifically recognize and neutralize antigens. Since the first monoclonal antibody was produced by hybridoma technology in 1975, it has become an important tool in both research and health care.
 
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                 To know the opinion from normal people, we started a questionnaire on WeChat.
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                 Here we developed a smart E.coli system for capturing B lymphocytes by utilizing the high affinity interaction between antigen and antibody. A peptide fused with RSA and GFP is expressed and purified as antigens for immunization and Elisa assay respectively, and this target peptide and an affinity tag are co-expressed on the surface of E.coli. Coupled with anti-tag magnetic beads, it can be used to screen and capture the desired B cells that secret the corresponding antibodies. These B cells can be fused or gene cloned for antibody producing. This system can also be applied to many aspects of enormous value, such as vaccine production and immune T-cell or tumor cell separation.
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According to the result, we found that most people only know a little or even none about B cells and Hybridoma technology. But after our introduction, most of them show their support to our project.
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Latest revision as of 19:31, 1 November 2017

Smart E.coli for B Cells Capture

Antibodies are Y-shaped proteins secreted by B lymphocytes that specifically recognize and neutralize antigens. Since the first monoclonal antibody was produced by hybridoma technology in 1975, it has become an important tool in both research and health care.

Here we developed a smart E.coli system for capturing B lymphocytes by utilizing the high affinity interaction between antigen and antibody. A peptide fused with RSA and GFP is expressed and purified as antigens for immunization and Elisa assay respectively, and this target peptide and an affinity tag are co-expressed on the surface of E.coli. Coupled with anti-tag magnetic beads, it can be used to screen and capture the desired B cells that secret the corresponding antibodies. These B cells can be fused or gene cloned for antibody producing. This system can also be applied to many aspects of enormous value, such as vaccine production and immune T-cell or tumor cell separation.

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