Difference between revisions of "Team:KAIT JAPAN/method"
| (7 intermediate revisions by the same user not shown) | |||
| Line 4: | Line 4: | ||
<h1><font color="#336EFF">METHOD</font></h1> | <h1><font color="#336EFF">METHOD</font></h1> | ||
<p>Genes used to create this system</p> | <p>Genes used to create this system</p> | ||
| − | <p><img src="https://static.igem.org/mediawiki/2017/6/6a/T--KAIT_JAPAN--IL-10R.png"height=" | + | <p><center><img src="https://static.igem.org/mediawiki/2017/6/6a/T--KAIT_JAPAN--IL-10R.png"height="280" width="400"></center></p> |
| − | + | <br> | |
| − | + | <br> | |
| − | + | <p><center><img src="https://static.igem.org/mediawiki/2017/f/fe/T--KAIT_JAPAN--jak.png"height="160 width="380"></center></p> | |
| − | + | <br> | |
| − | + | <br> | |
| − | + | <p><center><img src="https://static.igem.org/mediawiki/2017/c/c9/T--KAIT_JAPAN--stas1.png"height="200" width="380"></center></p> | |
| − | + | <br> | |
| − | + | <br> | |
| + | <p><center><img src="https://static.igem.org/mediawiki/2017/e/e9/T--KAIT_JAPAN--GFAP.png"height="200" width="380"></center></p> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <p><video width="800px" height="450px" autoplay loop> | ||
| + | <source src="https://static.igem.org/mediawiki/2017/d/d3/T--KAIT_JAPAN--video2.mp4" /> | ||
| + | <source src="https://static.igem.org/mediawiki/2017/f/ff/T--KAIT_JAPAN--video2.webm" /> | ||
| + | <img src="" alt="video2" /> | ||
| + | </video></p> | ||
| + | <p>When it is inserted into E.coli, and when IL-10 is received, same thing will occurs. IL-10 receptor is activated and STAT 1 phosphorylates. | ||
| + | Phosphorylated STAT1 will form a dimer, then binds to the GFAP promoter, and transcription starts.</p> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <p><center><img src="https://static.igem.org/mediawiki/2017/1/12/T--KAIT_JAPAN--membremprotein.png"height="350" width="600"></center></p> | ||
| + | <p>Therefore, to let IL-12 be transport out of the cell, gene is insert to the downstream of the GFAP promoter.</p> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <p><center><img src="https://static.igem.org/mediawiki/2017/4/4b/T--KAIT_JAPAN--gene.png"height="180" width="600"></center></p> | ||
| + | <p>HlyA is a signal peptide to carries IL-12 out of the cell.</p> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <p><video width="800px" height="450px" autoplay loop> | ||
| + | <source src="https://static.igem.org/mediawiki/2017/d/de/T--KAIT_JAPAN--video3.mp4" /> | ||
| + | <source src="https://static.igem.org/mediawiki/2017/2/20/T--KAIT_JAPAN--video3.webm" /> | ||
| + | <img src="" alt="video3" /> | ||
| + | </video></p> | ||
| + | <p>In this way, the IL-10 produced can be transported outside the cell.</p> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
Latest revision as of 19:33, 1 November 2017
METHOD
Genes used to create this system
When it is inserted into E.coli, and when IL-10 is received, same thing will occurs. IL-10 receptor is activated and STAT 1 phosphorylates. Phosphorylated STAT1 will form a dimer, then binds to the GFAP promoter, and transcription starts.
Therefore, to let IL-12 be transport out of the cell, gene is insert to the downstream of the GFAP promoter.
HlyA is a signal peptide to carries IL-12 out of the cell.
In this way, the IL-10 produced can be transported outside the cell.
Contact: kaitjapan@gmail.com Twitter:@KAIT_JAPAN
