Difference between revisions of "Team:CSU Fort Collins/Basic Part"

 
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h4>Note</h4>
 
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
 
  
  

Latest revision as of 19:39, 1 November 2017

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CSU iGEM



Basic Part

To developed pHF10, we used the Santangelo lab derived pLC71 as a chassis and used restriction enzymes and ligation techniques to insert the limonene synthase gene. For submission of a new part. our plans were to use a similar technique as the one used for the plasmid, except with pSB1C3. However, we had difficulty removing the limonene synthase gene from pHF10. This may be due to how we inserted it into the plasmid originally, or a complication from switiching plasmids.