Difference between revisions of "Team:Lethbridge HS/Model"

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<h2>&nbsp;Ink Production</h2>  
 
<h2>&nbsp;Ink Production</h2>  
<p class="center">&nbsp;The modeling portion of our project will allow us to estimate our pigment yields. The main pigment example that we are using is melanin. We borrowed from the Lagunas-Mu-oz et al-2006-Journal of Applied Microbiology and we learned that the yields of melanin in E. coli are the highest at a temperature of 30 degrees Celsius and at a pH of 7.0. Basing a model off of this will help us reach one of the highest melanin yields ever attained. We will be comparing our experimental results to theirs as well.
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<p class="center">&nbsp;The modeling portion of our project will allow us to estimate our pigment yields. The main pigment example that we are using is melanin. When designing the melA expression construct, we referenced from the Lagunas-Munoz et al and we learned that the yields of melanin in <i>E. coli</i> are the highest at a temperature of 30 degrees Celsius and at a pH of 7.0. We will be using their experimental results as a model for what we should be expecting during our attempts to produce the melA tyrosinase and melanin in <i>E. coli</i>.
 
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Revision as of 20:42, 1 November 2017





 Ink Production

 The modeling portion of our project will allow us to estimate our pigment yields. The main pigment example that we are using is melanin. When designing the melA expression construct, we referenced from the Lagunas-Munoz et al and we learned that the yields of melanin in E. coli are the highest at a temperature of 30 degrees Celsius and at a pH of 7.0. We will be using their experimental results as a model for what we should be expecting during our attempts to produce the melA tyrosinase and melanin in E. coli.




The graphs below show the highest results from the Lagunas-Munoz and colleagues.

This is a graph of the data of biomass (gDWCl¯¹) vs. time (hr) at a temperature of 30 degrees Celcius.   Sed vitae tellus lacus. Praesent enim nulla, ornare id est sed, condimentum aliquet erat. Donec ornare dapibus molestie. Nulla facilisi. Pellentesque sed lobortis ante. Morbi faucibus nulla non feugiat fringilla. Nunc augue neque, congue nec lectus eu, varius iaculis elit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam posuere tempus arcu eu mattis. Cras feugiat, enim non laoreet tempor, quam augue fringilla eros, vitae consequat augue dui sit amet ante. Morbi dignissim cursus augue. Cras dapibus lobortis volutpat. Maecenas felis ipsum, pharetra eget pulvinar ut, efficitur laoreet est. Aenean ullamcorper fringilla ligula nec posuere.

This is a graph of the data of glucose levels (g l¯¹) vs. time (hr) at a temperature of 30 degrees Celcius   Sed vitae tellus lacus. Praesent enim nulla, ornare id est sed, condimentum aliquet erat. Donec ornare dapibus molestie. Nulla facilisi. Pellentesque sed lobortis ante. Morbi faucibus nulla non feugiat fringilla. Nunc augue neque, congue nec lectus eu, varius iaculis elit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam posuere tempus arcu eu mattis. Cras feugiat, enim non laoreet tempor, quam augue fringilla eros, vitae consequat augue dui sit amet ante. Morbi dignissim cursus augue. Cras dapibus lobortis volutpat. Maecenas felis ipsum, pharetra eget pulvinar ut, efficitur laoreet est. Aenean ullamcorper fringilla ligula nec posuere.

This is a graph of the data of eumelanin (g l¯¹) vs. time (hr) at a temperature of 30 degrees Celcius   Sed vitae tellus lacus. Praesent enim nulla, ornare id est sed, condimentum aliquet erat. Donec ornare dapibus molestie. Nulla facilisi. Pellentesque sed lobortis ante. Morbi faucibus nulla non feugiat fringilla. Nunc augue neque, congue nec lectus eu, varius iaculis elit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam posuere tempus arcu eu mattis. Cras feugiat, enim non laoreet tempor, quam augue fringilla eros, vitae consequat augue dui sit amet ante. Morbi dignissim cursus augue. Cras dapibus lobortis volutpat. Maecenas felis ipsum, pharetra eget pulvinar ut, efficitur laoreet est. Aenean ullamcorper fringilla ligula nec posuere.

This is a graph of the data of eumelanin (g l¯¹) vs. time (hr) at a temperature of 30 degrees Celcius   Sed vitae tellus lacus. Praesent enim nulla, ornare id est sed, condimentum aliquet erat. Donec ornare dapibus molestie. Nulla facilisi. Pellentesque sed lobortis ante. Morbi faucibus nulla non feugiat fringilla. Nunc augue neque, congue nec lectus eu, varius iaculis elit. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Nam posuere tempus arcu eu mattis. Cras feugiat, enim non laoreet tempor, quam augue fringilla eros, vitae consequat augue dui sit amet ante. Morbi dignissim cursus augue. Cras dapibus lobortis volutpat. Maecenas felis ipsum, pharetra eget pulvinar ut, efficitur laoreet est. Aenean ullamcorper fringilla ligula nec posuere.