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| | {{Shanghaitech/js}} | | {{Shanghaitech/js}} |
| | <html> | | <html> |
| − | | + | <style> |
| − | <div id='main-content-wrapper'> | + | .ol-step-i li{ |
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| | <div class="column half_size" id="content-block"> | | <div class="column half_size" id="content-block"> |
| | + | <!--content begins here COMMENT LANDMARK--> |
| | + | <div id="This-is-where-it-begins" style="padding: 10% 20% 0 20%;background:url('https://static.igem.org/mediawiki/2017/2/22/T--Shanghaitech--interlawuxianb2.jpg') no-repeat 50% 0%/ contain;"> |
| | <h1>Hardware</h1> | | <h1>Hardware</h1> |
| − | <div class="column half_size"> | + | <h2>Introduction</h2> |
| − | <p class="MsoNormal" align="center" style="text-align:center"><!--[if gte vml 1]><v:shapetype | + | <p>To lower the barrier of synthetic biology to the public, we aimed to develop an integrated hardware platform that allows building a complex bio-product free of lab work and with minimal requirement of experience in synthetic biology for the designers. This platform comprises of an interface to a cloud server, a special tube to culture bacteria, and a liquid-handling robot to transfer liquid. Upon receiving the command, the gene circuits to produce a bio-product through a cloud server, bacteria representing the blocks in gene circuits will be inoculated into our special tube. The tube can separate signal molecule-containing supernatant from bacteria cell mass. The supernatant is transferred as desired to another tube through our robot, thus connecting two blocks in the circuits. With delicate gene circuits, a bio-product is produced. Therefore, the designers produce a bio-product without visiting bio-labs. We have built a low cost prototype hardware platform and showed it functioned properly.</p> |
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| + | <p>Here is a brief introduction of our hardware design. With our proper design and manufacture, we can make our prototype hardware within 1 day and with less than $180, which is very cost effective.</p> |
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| + | |
| − | font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Hardware</span></b><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:"Arial Unicode MS""><o:p></o:p></span></b></p>
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| | | | |
| − | <p class="MsoNormal"><b><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Introduction:<o:p></o:p></span></b></p> | + | <br><br> |
| | + | <h2>A. Interface with a cloud server.</h2> |
| | + | <p>As shown in the demo video, we have successfully built the prototype hardware platform and verified that this platform could be controlled remotely through our <a href="https://2017.igem.org/Team:Shanghaitech/Software">software interface</a>. It will work autonomously after receiving proper commands via internets or a cloud server. ($120) |
| | + | </p> |
| | + | <h2>B. Special tube to culture bacteria.</h2> |
| | + | <ol style="list-style: none"> |
| | | | |
| − | <p class="MsoNormal" style="text-indent:21.3pt;mso-char-indent-count:2.24"><span lang="EN-US" style="font-size: 9.5pt; font-family: Arial, sans-serif;">To lower the barrier of | + | <li><h2>Design</h2></li> |
| − | synthetic biology to the public, we aimed to develop an integrated hardware
| + | <p>Our magic blocks are bacteria cultures with desired information processing ability. To connect bacteria with clear logic rules, bacteria need them to communicate with each other according to gene circuits designed by the designers. We used quorum sensing system to communicate one bacteria population with another. The secreted signal chemicals need to be transferred between bacteria to induce further signal production. However, the transfer of bacteria cells should be avoided so as to reduce the interference between different bacteria populations. Therefore, we need to design a special culture tube (Fig.B) so that the culture supernatant can be easily sucked away and cells will stay in the original tubes. As shown below, to make our special tube, we glued two tubes together separated by a 0.22um membrane so that the bacteria will stay in the larger tube outside and signal chemicals can diffuse into the smaller tube inside. The supernatant inside of the smaller tube can be transferred out by our liquid handling robot as needed.</p> |
| − | platform that allows building a complex bio-product free of lab work and with
| + | <div class="image-text-container image-text-centered"> |
| − | minimal requirement of experience in synthetic biology for the designers. This
| + | <p class=" image-container"><img src="https://static.igem.org/mediawiki/2017/ c/ c0/T--Shanghaitech-- Hardware-- bacteria- culture- tube. png" alt=" special bacteria culture tube" width=" 1249" height=" 1408"/></p> |
| − | platform comprises of an interface to a cloud server, a special tube to culture
| + | <p>Fig.B The design of the special bacteria culture tube. Only supernatant that contains signal molecules can be transferred out to initiate further responses.</p> |
| − | bacteria, and a liquid-handling robot to transfer liquid. Upon receiving the
| + | |
| − | command, the gene circuits to produce a bio-product through a cloud server,
| + | |
| − | bacteria representing the blocks in gene circuits will be inoculated into our
| + | |
| − | special tube. The tube can separate signal molecule-containing supernatant from
| + | |
| − | bacteria cell mass. The supernatant is transferred as desired to another tube
| + | |
| − | through our robot, thus connecting two blocks in the circuits. With delicate
| + | |
| − | gene circuits, a bio-product is produced. Therefore, the designers produce a
| + | |
| − | bio-product without visiting bio-labs. We have built a low cost prototype
| + | |
| − | hardware platform and showed it functioned properly.<o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p class="MsoNormal" style="text-indent:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Here
| + | |
| − | is a brief introduction of our hardware design. With our proper design and manufacture,
| + | |
| − | we can make our prototype hardware within 1 day and with less than $180, which
| + | |
| − | is very cost effective. <o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p class="MsoNormal" style="text-indent:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Video:<o:p></o:p></span></p>
| + | |
| − | | + | |
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| − | mso-font-kerning:0pt"><o:p></o:p></span></p>
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| − | <p class="MsoNormal" style="margin-left:18.0pt;text-indent:-18.0pt;mso-list:l2 level1 lfo1"><!--[if !supportLists]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:Arial">A.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";"> </span></span></b><!--[endif]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:"Arial Unicode MS"">Interface with a cloud server.<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p class="MsoNormal" style="margin-left:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">As
| + | |
| − | shown in the demo video, we have successfully built the prototype hardware
| + | |
| − | platform and verified that this platform could be controlled remotely through our
| + | |
| − | software interface (</span><span lang="EN-US"><a href="https://2017.igem.org/Team:Shanghaitech/Software"><span style="font-family:
| + | |
| − | "Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">https://2017.igem.org/Team:Shanghaitech/Software</span></a></span><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">).
| + | |
| − | It will work autonomously after receiving proper commands via internets or a
| + | |
| − | cloud server.<o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p class="MsoNormal" style="margin-left:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS""> </span></p>
| + | |
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| + | |
| − | mso-fareast-font-family:Arial">B.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";"> </span></span></b><!--[endif]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:"Arial Unicode MS"">Special tube to culture bacteria.<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:21.0pt;text-indent:-21.0pt;
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| − | mso-char-indent-count:0;mso-list:l1 level1 lfo2"><!--[if !supportLists]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:Arial">1.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";"> </span></span></b><!--[endif]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
| + | |
| − | mso-fareast-font-family:"Arial Unicode MS"">Design:<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p class="MsoNormal" style="text-indent:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Our | + | |
| − | magic blocks are bacteria cultures with desired information processing ability. | + | |
| − | To connect bacteria with clear logic rules, bacteria need them to communication | + | |
| − | with each other according to gene circuits designed by the designers. We used | + | |
| − | quorum sensing system to communicate one bacteria population with another. The | + | |
| − | secreted signal chemicals need to be transferred between bacteria to induce | + | |
| − | further signal production. However, the transfer of bacteria cells should be | + | |
| − | avoided so as to reduce the interference between different bacteria | + | |
| − | populations. Therefore, we need to design a special culture tube<span style="color:red"> </span>(Fig.B) so that the culture supernatant can be easily | + | |
| − | sucked away and cells will stay in the original tubes. As shown below, to make | + | |
| − | our special tube, we glued two tubes together separated by a 0.22um membrane so | + | |
| − | that the bacteria will stay in the larger tube outside and signal chemicals can | + | |
| − | diffuse into the smaller tube inside. The supernatant inside of the smaller | + | |
| − | tube can be transferred out by our liquid handing robot as needed. <o:p></o:p></span></p> | + | |
| − | | + | |
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| − | <td><img width="250" height="282" src="https://static.igem.org/mediawiki/2017/ 8/ 88/T--Shanghaitech-- hyp3hardware.jpg" v:shapes="_x0000_s1026"></td> | + | |
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| − | <p class="MsoNormal" style="text-indent:18.0pt"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Fig.B
| + | |
| − | The design of the special bacteria culture tube. Only supernatant that contains | + | |
| − | signal molecules can be transferred out to initiate further responses. <o:p></o:p></span></p> | + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:21.0pt;text-indent:-21.0pt;
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| − | mso-char-indent-count:0;mso-list:l1 level1 lfo2"><!--[if !supportLists]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
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| − | mso-fareast-font-family:Arial">2.<span style="font-variant-numeric: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";"> </span></span></b><!--[endif]--><b><span lang="EN-US" style="font-family:"Arial",sans-serif;
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| − | mso-fareast-font-family:"Arial Unicode MS"">Manufacture<o:p></o:p></span></b></p>
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| − | <p class="MsoNormal"><span lang="EN-US" style="font-family:"Arial",sans-serif;
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| − | mso-fareast-font-family:"Arial Unicode MS""> Specific steps:<o:p></o:p></span></p>
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| − | width:30px;height:40px"><img width="24" height="32" src="file:///C:/Users/%E8%83%A1%E5%B0%9A%E5%BF%97/AppData/Local/Temp/msohtmlclip1/01/clip_image010.png" v:shapes="下箭头_x0020_14"></span><!--[endif]--><span lang="EN-US" style="font-family:
| + | |
| − | "Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Step2: Apply a
| + | |
| − | layer of glue evenly to the exposed cross section of cut and then glue a piece
| + | |
| − | of 0.22 um membrane to it. Apply additional layer of glue to strengthen the
| + | |
| − | attachment between the membrane and the cross section.<o:p></o:p></span></p>
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| | | | |
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| + | <img class="image-left" src="https://static.igem.org/mediawiki/2017/f/ff/T--Shanghaitech--Hardware--Cut-position.jpg" alt="Cut position of a 15 ml tube" width="493" height="277" /> |
| − | left:0;text-align:left;margin-left:25.25pt;margin-top:8.4pt;width:113.5pt;
| + | <span class="text-wrapper"><p>Step1: Cut a 15 ml centrifuge tube at the position of 0.6 ml. 5s pre-heating to soft the tube is recommended.</p></span> |
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| + | |
| − | <td width="48" height="423"></td>
| + | |
| − | </tr>
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| − | <tr>
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| − | <td></td>
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| − | <td width="193" height="73" style="vertical-align:top"><!--[endif]--><!--[if !mso]--><span style="position:absolute;mso-ignore:vglayout;left:0pt;z-index:251677184">
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| + | |
| − | <p class="MsoNormal" align="left"><span lang="EN-US" style="color:yellow">Sealed tube with 0.22um membrane<o:p></o:p></span></p>
| + | |
| | </div> | | </div> |
| − | <!--[if !mso]--></td> | + | <div class="image-text-container image-text-centered"><p><img src="https://static.igem.org/mediawiki/2017/b/b6/T--Shanghaitech--Hardware--Arrow-down.png" alt="Arrow Down" height="140" width="100" /></p></div> |
| − | </tr>
| + | <div class="image-text-container image-left"> |
| − | </tbody></table>
| + | <img class=" image-left" src=" https:// static.igem.org/ mediawiki/ 2017/ 2/ 22/ T-- Shanghaitech-- Hardware-- Sealed-tube.jpg" alt=" Sealed tube with 0. 22um membrane" width=" 670" height=" 377" /> |
| − | </span><!--[endif]--><!--[if !mso & !vml]--> <!--[endif]--><!--[if !vml]--></td>
| + | <span class="text-wrapper"><p>Step2: Apply a layer of glue evenly to the exposed cross section of cut and then glue a piece of 0.22 um membrane to it. Apply additional layer of glue to strengthen the attachment between the membrane and the cross section.</p></span> |
| − | </tr>
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| + | |
| − | "Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Step3: In the
| + | |
| − | lid center of a 50 ml centrifuge tube, make a hole so that the 15 ml tube can
| + | |
| − | pass by. And then fix the 15 ml tube inside of the 50 ml tube with glue as
| + | |
| − | showing in the left. Now the bacteria can be culture in the 50 ml tube and the
| + | |
| − | signal containing supernatant can be removed from the 15 ml tube.<o:p></o:p></span></p>
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| + | < span class= "text- wrapper"><p> Step3: In the lid center of a 50 ml centrifuge tube , make a hole so that the 15 ml tube can pass by. And then fix the 15 ml tube inside of the 50 ml tube with glue as showing in the left. Now the bacteria can be culture in the 50 ml tube and the signal containing supernatant can be removed from the 15 ml tube.</p></span> |
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| − | <p class="MsoListParagraph" style="margin-left:63.0pt;mso-char-indent-count:0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS""> </span></p>
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| − | mso-fareast-font-family:"Arial Unicode MS"">Liquid-handling robot<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:60.0pt;text-indent:-18.0pt;
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| − | mso-fareast-font-family:"Arial Unicode MS"">Design<o:p></o:p></span></b></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:60.0pt;text-indent:0cm;mso-char-indent-count:
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| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
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| − | "Arial Unicode MS"">Considering the cost and manufacture difficulties, we decided
| + | |
| − | to DIY a liquid handling robot by reconfiguration of a 3D printer together with
| + | |
| − | a syringe pump. The ready-made interface port in the printer allows automatic
| + | |
| − | and remote control. We therefore can program the 3D printer rig to move the
| + | |
| − | syringe pump to suck and transfer liquid from the special tube we designed
| + | |
| − | above. <o:p></o:p></span></p>
| + | |
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| − | mso-fareast-font-family:"Arial Unicode MS"">Manufacture<o:p></o:p></span></b></p>
| + | |
| − | | + | |
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| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
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| − | "Arial Unicode MS"">Step1: </span><span lang="EN-US" style="mso-bidi-font-size:
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| − | 10.5pt;font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">Get
| + | |
| − | an X-Y-Z <span style="color:#2E3033;background:#F9FBFC">3 axes programmable platform</span></span><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">
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| + | |
| − | bought an anycubic-i3 3D printer for reconfiguration from Taobao: </span><span lang="EN-US"><a href="https://item.taobao.com/item.htm?id=530445909316"><span style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">https://item.taobao.com/item.htm?id=530445909316</span></a></span><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS""><o:p></o:p></span></p>
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| | | | |
| − | </span></p><table cellpadding="0" cellspacing="0" align="left"> | + | <h2>C. Liquid-handling robot</h2> |
| − | <tbody><tr>
| + | <ol> |
| − | <td width="0" height="0"></td>
| + | <h2>Design</h2> |
| − | <td width="478"></td>
| + | <p>Considering the cost and manufacture difficulties, we decided to DIY a liquid handling robot by reconfiguration of a 3D printer together with a syringe pump. The ready-made interface port in the printer allows automatic and remote control. We therefore can program the 3D printer rig to move the syringe pump to suck and transfer liquid from the special tube we designed above.</p> |
| − | <td width="28"></td>
| + | <h2>Manufacture</h2> |
| − | <td width="416"></td>
| + | <ol class="ol-step-i"> |
| − | </tr>
| + | <li>Get an X-Y-Z 3 axes programmable platform</li> |
| − | <tr>
| + | <p>We bought an anycubic-i3 3D printer for reconfiguration from Taobao: </p><p>(<a href="https://item.taobao.com/item.htm?id=530445909316">https://item.taobao.com/item.htm?id=530445909316</a>)</p> |
| − | <td height="3"></td>
| + | <div class="two-columns-img-text"> |
| − | <td colspan="2"></td>
| + | <span> <img src="https://static.igem.org/mediawiki/2017/9/94/T--Shanghaitech--hyphardware7.png" alt="DIY components" width="729" height="431"\> </span> |
| − | <td rowspan="2" align="left" valign="top"><img width="333" height="257" src="file:///C:/Users/%E8%83%A1%E5%B0%9A%E5%BF%97/AppData/Local/Temp/msohtmlclip1/01/clip_image019.png" alt="DSC09227" v:shapes="图片_x0020_42 _x0000_s1046"></td>
| + | <span> <img src="https://static.igem.org/mediawiki/2017/1/19/T--Shanghaitech--hyphardware8.jpg" alt="Assembled Rig" width="941" height="529"\> </span> |
| − | </tr>
| + | </div> |
| − | <tr>
| + | <div class="two-columns-img-text"> |
| − | <td height="318"></td>
| + | <span> <p>DIY Components</p> </span> |
| − | <td align="left" valign="top"><img width="382" height="254" src="file:///C:/Users/%E8%83%A1%E5%B0%9A%E5%BF%97/AppData/Local/Temp/msohtmlclip1/01/clip_image020.png" v:shapes="图片_x0020_12 _x0000_s1047"></td>
| + | <span> <p>Assembled Rig</p> </span> |
| − | </tr>
| + | </div> |
| − | </tbody></table> | + | |
| | | | |
| − | <!--[endif]--><span lang="EN-US" style="font-family:"Arial",sans-serif; | + | <li>Assemble a syringe pump</li> |
| − | mso-fareast-font-family:"Arial Unicode MS""> </ span><p> </p> | + | <p> To assemble a syringe pump, we bought a screw rod ($1. 5) to mount an extruder’s stepping motor with its coupler |
| − | | + | (<a href="https://item.taobao.com/item.htm?id=16191694967">https://item.taobao.com/item.htm?id=16191694967</a>) 5mm transfer to 8mm $3.2.</p> |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count:
| + | <div class="two-columns-img-text"> |
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| + | <span> |
| − | "Arial Unicode MS""> </span></p>
| + | <img src="https://static.igem.org/mediawiki/2017/5/5d/T--Shanghaitech--hyphardware9.png" alt="screw rod" width="543" height="55"\> |
| − | | + | </span> |
| − | <p class="MsoListParagraph"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS""> </span></p>
| + | <span> |
| − | | + | <div class="two-columns-img-text"> |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count:
| + | <span><img src="https://static.igem.org/mediawiki/2017/5/57/T--Shanghaitech--hyphardware10.png" alt="coupler" width="163" height="126"\></span> |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | <span><img src="https://static.igem.org/mediawiki/2017/9/9d/T--Shanghaitech--hyphardware11.png" alt="stepping motor" width="245" height="237"\></span> |
| − | "Arial Unicode MS""> </span></p>
| + | </div> |
| − | | + | </span> |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count: | + | </div> |
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| + | <div class="two-columns-img-text"> |
| − | "Arial Unicode MS""> </span></p>
| + | <span> |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count: | + | </span> |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | <span> |
| − | "Arial Unicode MS""> </span></p>
| + | <div class="two-columns-img-text"> |
| − | | + | <span><p> coupler< /p>< /span > |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count:
| + | <span><p> stepping motor< /p>< /span > |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | </div> |
| − | "Arial Unicode MS""> </span></p>
| + | </span> |
| − | | + | </div> |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count: | + | <div class="image-text-container image-text-centered"> |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | <p class="image-container"><img src="https://static.igem.org/mediawiki/2017/9/95/T--Shanghaitech--hyphardware12.jpg" alt="assembled syringe pump" width="1211" height="681"/></p> |
| − | "Arial Unicode MS""> </span></p>
| + | <p> Assembled syringe pump</p> |
| − | | + | </div> |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count:
| + | <li>Mount the syringe pump to the 3D printer</li> |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | <p> Attach the syringe’s pushrod with the nut of screw rod. Link the syringe to the plastic tubing on the mobile platform.</ p> |
| − | "Arial Unicode MS""> </span></p>
| + | <div class="image-text-container image-text-centered"> |
| − | | + | <p class="image-container"><img src="https://static.igem.org/mediawiki/2017/4/45/T--Shanghaitech--Hardware--attaching.jpg" alt="Attaching syringe to the pump" width="878" height="494"/></p> |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count: | + | |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | |
| − | "Arial Unicode MS""> </span></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count:
| + | |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | |
| − | "Arial Unicode MS""> </span></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count: | + | |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | |
| − | "Arial Unicode MS""> </span></p>
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| − | | + | |
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| + | |
| − | "Arial Unicode MS""> </span></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count: | + | |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | |
| − | "Arial Unicode MS""> </span></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count: | + | |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | |
| − | "Arial Unicode MS""> </span></p>
| + | |
| − | | + | |
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| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count: | + | |
| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| + | |
| − | "Arial Unicode MS"">Step2: assemble a syringe pump<o:p></o:p></span></p>
| + | |
| − | | + | |
| − | <p class="MsoListParagraph" style="margin-left:42.0pt;mso-char-indent-count:0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">To | + | |
| − | assemble a syringe pump, we bought a screw rod ($1.5) to mount an extruder’s stepping motor with its coupler (</span><span lang="EN-US"><a href="https://item.taobao.com/item.htm?id=16191694967"><span style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">https://item.taobao.com/item.htm?id=16191694967</span></a></span><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">
| + | |
| − | 5mm transfer to 8mm $3.2).<o:p></o:p></span></p>
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| | | | |
| − | </span></p><table cellpadding="0" cellspacing="0" align="left"> | + | <li>Program the 3D printer and the syringe</li> |
| − | <tbody><tr>
| + | <p>Any serial assistant software outputting G-code commands can control the 3D printer and the syringe pump. We used the pronterface (<a href="http://www.pronterface.com/">http://www.pronterface.com/</a>) with baud rate 250000 to control both. Now the assembled platform with the 3D printer and the syringe pump is in action. The 3D printer rig can move the suction tip to desired tubes specified by gene circuits. Then the pump can suck the supernatant via the suction tip and transfer liquid to another tube to initiate further signaling.</p> |
| − | <td width="0" height="0"></td>
| + | <div class="image-text-container image-text-centered"> |
| − | <td width="52"></td>
| + | < p class=" image-container"><img src=" https:// static. igem.org/ mediawiki/ 2017/ 4/ 4c/ T-- Shanghaitech-- hyphardware16.png" alt=" the pronterface window" width=" 904" height=" 465" />< /p> |
| − | <td width="76"></td>
| + | <p> The pronterface window</p> |
| − | <td width="168"></td>
| + | |
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| + | |
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| + | |
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| + | |
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| + | |
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| + | |
| − | <td width="166"></td>
| + | |
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| − |
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| − |
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| |
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| − |
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| − |
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| − | <p class="MsoListParagraph" style="margin-left:42.0pt;mso-char-indent-count:0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS""> </span></p>
| |
| − |
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| − | <p class="MsoListParagraph" style="margin-left:42.0pt;mso-char-indent-count:0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS""> </span></p>
| |
| − |
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| − | <br clear="ALL">
| |
| − |
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| − | <p class="MsoListParagraph" style="margin-left:42.0pt;text-indent:0cm;mso-char-indent-count:
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| − | 0"><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:
| |
| − | "Arial Unicode MS"">Step3: Mount the syringe pump to the 3D printer. <o:p></o:p></span></p>
| |
| − |
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| |
| − | the syringe’s pushrod with the nut of screw rod. Link the syringe to the plastic tubing on the mobile platform. <o:p></o:p></span></p>
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| |
| − | the 3D printer and the syringe.<o:p></o:p></span></b></p>
| |
| − |
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| |
| − | serial assistant software outputing G-code commands can control the 3D printer and the syringe pump. We used
| |
| − | the pronterface (</span><span lang="EN-US"><a href="http://www.pronterface.com/"><span style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">http://www.pronterface.com/</span></a></span><span lang="EN-US" style="font-family:"Arial",sans-serif;mso-fareast-font-family:"Arial Unicode MS"">)
| |
| − | with baud rate 250000 to control both. Now the assembled platform with the 3D
| |
| − | printer and the syringe pump is in action. The 3D printer rig can move the suction
| |
| − | tip to desired tubes specified by gene circuits. Then the pump can suck the
| |
| − | supernatant via the suction tip and transfer liquid to another tube to initiate
| |
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