Difference between revisions of "Team:Cadets2Vets"

 
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{{Cadets2Vets}}
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{{Team:Cadets2Vets/SideMenu}}
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<!-- FORM START -->
 +
<div  id="vbid-59a5b-tjgooy1n"  class="master item-box  page-box style-59a5b-ti3xte4k    button-effects btn_hover3      " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-ti3xte4k" data-preview-styleid='style-59a5b-ti3xte4k' data-preset-type-id="FORM">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="page-wrapper item-wrapper form-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-tjgooy1n" data-preview-style="style-59a5b-ti3xte4k" data-style="style-e4cfb-6huavktiba" data-orig-thumb-height="2416" data-orig-thumb-width="3500" data-vbid="vbid-59a5b-tjgooy1n" data-bgimg="https://static.igem.org/mediawiki/2017/7/7c/Army_in_Parade_Dress.jpeg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-59a5b-nnn4mbrq-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
 +
<div  id="vbid-59a5b-nnn4mbrq" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/7/7c/Army_in_Parade_Dress.jpeg);"  data-orig-width="3500" data-orig-height="2416" >
 +
<!-- VIDEO TEMPLATE -->
 +
  
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
−
<div class="column full_size" >
 
−
<h1> Welcome to iGEM 2017! </h1>
 
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
 
−
</div>
 
  
−
<div class="clear"></div>
+
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
−
<div class="column half_size" >
+
<!-- RAW TEMPLATE -->
−
<h5>Before you start: </h5>
+
−
<p> Please read the following pages:</p>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<ul>
+
 
−
<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
+
</div>
−
<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
−
<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
+
−
</ul>
+
 
</div>
 
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<div class="preview-icon-holder Icon removable-parent order-handle" >
 +
 +
<img id="element-2e144876c8f0e64" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://static.igem.org/mediawiki/2017/thumb/d/d2/Cadets2Vets_Logo.jpg/800px-Cadets2Vets_Logo.jpg.png" />
 +
 +
</div>
  
−
<div class="column half_size" >
+
−
<div class="highlight">
+
−
<h5> Styling your wiki </h5>
+
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 
</div>
 
</div>
 
</div>
 
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
<!-- FORM END -->
 +
 +
 +
 +
 +
<!-- MAPS START -->
 +
<div  id="vbid-59a5b-qihkumq9"  class="master item-box  gallery-box style-59a5b-jqfr7efo    button-effects btn_hover3      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-jqfr7efo" data-preview-styleid='style-59a5b-jqfr7efo' data-preset-type-id="MAPS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper maps-wrapper ">
 +
 +
<div  class="sub container style-59a5b-jqfr7efo content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-59a5b-jqfr7efo" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-59a5b-qihkumq9" data-preset-type-id="MAPS" data-preview-style="style-59a5b-jqfr7efo" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="AUTOPLAY"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-59a5b-ghvxgbnu"  class="sub item-box  page-box style-59a5b-jqfr7efo          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-jqfr7efo" data-preview-styleid='style-59a5b-jqfr7efo' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-ghvxgbnu" data-preview-style="style-59a5b-jqfr7efo" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-ghvxgbnu" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div class="column full_size" >
+
<div id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<h5> Wiki template information </h5>
+
<!-- VIDEO TEMPLATE -->
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
  
−
</div>  
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
  
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
−
<div class="column half_size" >
+
</div>
−
<h5> Editing your wiki </h5>
+
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
+
</div>
−
<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<div class="preview-icon-holder Icon removable-parent order-handle" >
 +
 +
<img id="vbid-59a5b-qgicu05p" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://static.igem.org/mediawiki/2017/3/30/US_Army_Logo.jpg" />
 +
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-59a5b-j6hw2jzr" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >WE ARE Cadets2Vets.</h2>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="vbid-59a5b-potfnoot" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, <br>Tacoma, WA 98402<br></h3>
  
−
<div class="column half_size" >
 
−
<h5>Tips</h5>
 
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
−
<ul>
 
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
−
<li>Be clear about what you are doing and how you plan to do this.</li>
 
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
 
−
<li>Have lots of fun! </li>
 
−
</ul>
 
 
</div>
 
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
  
 +
<span id="vbid-59a5b-hgd5ta77"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
  
−
<div class="column half_size" >
 
−
<h5>Inspiration</h5>
 
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
−
<ul>
 
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
−
</ul>
 
 
</div>
 
</div>
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-59a5b-ma3fusho"  class="sub item-box  page-box style-59a5b-jqfr7efo          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-jqfr7efo" data-preview-styleid='style-59a5b-jqfr7efo' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-ma3fusho" data-preview-style="style-59a5b-jqfr7efo" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="2952" data-orig-thumb-width="5248" data-vbid="vbid-59a5b-ma3fusho" data-bgimg="https://static.igem.org/mediawiki/2017/8/8c/Group_Photo.jpgC8tBbDNwQs5kZXRkYWwbVMMpLNUA">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-59a5b-2w1tabau-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div class="column half_size" >
+
<div id="vbid-59a5b-2w1tabau" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/8/8c/Group_Photo.jpg);" data-orig-width="5248" data-orig-height="2952" >
−
<h5> Uploading pictures and files </h5>
+
<!-- VIDEO TEMPLATE -->
−
<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
−
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
+
  
−
<a href="https://2017.igem.org/Special:Upload">
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
UPLOAD FILES
+
 
−
</a>
+
 
−
</p>
+
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 
</div>
 
</div>
 +
  
−
<head>
+
</div>
−
    <meta charset="UTF-8">
+
</div>
−
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
+
<div class="text-side shrinker-parent">
−
    <meta name="viewport" content="width=device-width, initial-scale=1">
+
<div class="vertical-aligner">
−
    <!-- The above 3 meta tags *must* come first in the head; any other head content must come *after* these tags -->
+
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
−
    <title>iGEM : Integrated practices</title>
+
<div class="draggable-div-holder"></div>
−
    <!-- Favicon -->
+
<div class="preview-content-holder shrinker-content">
−
    <link rel="shortcut icon" type="image/icon" href="https://static.igem.org/mediawiki/2016/6/6b/T--INSA-Lyon--favicon.png"/>
+
−
    <!-- Font Awesome -->
+
<!-- BY SPECIFIC ORDER -->
−
    <link href="https://2016.igem.org/Template:INSA-Lyon/font-awesome?action=raw&amp;ctype=text/css" rel="stylesheet">
+
−
    <!-- Bootstrap -->
+
−
    <link href="https://2016.igem.org/Template:INSA-Lyon/bootstrap?action=raw&amp;ctype=text/css" rel="stylesheet">
+
−
    <!-- Theme color -->
+
−
    <link id="switcher" href="https://2016.igem.org/Template:INSA-Lyon/CSS?action=raw&amp;ctype=text/css" rel="stylesheet">
+
−
    <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Team:INSA-Lyon/css_global?action=raw&amp;ctype=text/css" />
+
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- MAPS END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-b282ff56-nm8gak7e"  class="master item-box  gallery-box style-b282ff56-lz7anfvy    button-effects btn_hover3      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="NATIVE_ORDER"  data-styleid="style-b282ff56-lz7anfvy" data-preview-styleid='style-b282ff56-lz7anfvy' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-b282ff56-lz7anfvy content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-b282ff56-lz7anfvy" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-b282ff56-nm8gak7e" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-b282ff56-lz7anfvy" data-style="style-b82c0-fvpabxxvdo" data-absolute-path="" >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="matrix"
 +
data-ARRANGER_COLS="3"
 +
data-ARRANGER_ITEM_MAX_WIDTH="500"
 +
data-ARRANGER_ITEM_MIN_WIDTH="490"
 +
data-ARRANGER_ITEM_RATIO="0.4"
 +
data-ARRANGER_ITEM_SPACING="10"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-b282ff56-hpsoubkd"  class="sub item-box  page-box style-b282ff56-lz7anfvy          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-b282ff56-lz7anfvy" data-preview-styleid='style-b282ff56-lz7anfvy' data-preset-type-id="UNRESOLVED">
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<p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><br></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span id="docs-internal-guid-07d9d787-1726-69e0-e827-dac88147f21b"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">As outlined by measure 7061A from the </span><span style="font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">Environmental Protection Agency</span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">, atomic absorption/gaseous hydride is the current method for arsenic detection in wastes, soils, and groundwater. The method begins with a nitric or sulfuric acid digest. Using tin chloride, the arsenic is reduced into its trivalent state and converted into a volatile hydride. The hydride is then swept into an argon-hydrogen flame.The subsequent radiation is read by a spectrophotometer; the result is proportional to the arsenic concentration in the environmental sample. This process requires highly trained technicians and a significant delay for laboratory proceedings to be completed, costing up to $150 per sample. </span></span></span></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><br></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span id="docs-internal-guid-07d9d787-1726-69e0-e827-dac88147f21b"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Evidently, the acid digest method for arsenic detection is simply not reasonable for the impoverished people of Bangladesh, who face a severe crisis in arsenic contamination. It is estimated that nearly 57 million citizens of the Bengal basin are currently consuming water that exceeds the World Health Organization’s standard of arsenic concentration at ten parts per billion (Henke, 2009). WHO investigators estimate that more than 20% of deaths in Bangladesh could be attributed to dangerous concentrations of arsenic in tube wells. Additionally, the United States military remains concerned about exposure of troops to unsafe environmental conditions,
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including arsenic-contaminated drinking water. These issues show an urgent need for a field-effective,
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inexpensive sensor for arsenic.</span></span></span></p>
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                            <h2 class="title">Integrated Human Practices</h2>
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                                Our human practices reflection is based on the <strong>interactions</strong> between the conception of our <strong>device</strong> and the <strong>society</strong>. How could our work be integrated in the prevention of STIs? The goal was to conduct a rigorous <strong>reflexion on the project</strong>. First we used the knowledge acquired during <a href="https://2016.igem.org/Team:INSA-Lyon/Engagement">meetings and surveys</a> we carried to build an ethical matrix.
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                                </br>The help provided by our human sciences instructors was very valuable as they guided us through interesting ways of thinking.
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                                    <img src="https://static.igem.org/mediawiki/2016/4/48/T--INSA-Lyon--matricePreview.png" class="img-responsive"><a target="_blank" href="https://static.igem.org/mediawiki/2016/8/80/T--INSA-Lyon--matrice.pdf">Ethical matrix - Click to enlarge</a>
 
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                                Once the matrix was established, on the one hand, we confronted it with the <strong>results of the survey</strong> in the matrix synthesis.
 
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                                </br>On the other hand, it was used as an instrument to have a <strong>human overview of the project</strong> to take a needed step back as everyone was focused on its parts. An ethical matrix was a useful framework to assist us in our deliberation about technical choices but also social choices.
 
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                                The matrix synthesis was realized to have a humanist point of view integrated into our project. In this perspective, we used the approach of the ethic of care (Joan Tronto, <span style="font-style:italic">Moral boundaries: a political argument for ethic of care</span>, 1993) to interpret our results.
 
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                                </br>We identified 4 statements: <strong>innovation, perception, risk, responsibility</strong> to improve our project according to two phases of the ethic of care.
 
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                                The <strong>innovation</strong> deals with <strong>human consequences of our technology</strong>.
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                                </br>Early detection, short delivery time of results, low cost impact the use of the diagnosis. The early detection allows to improve patient's care, thus the treatment can begin very early. The short delivery time of results is compatible with mobile prevention campaign and emergency situations. Low cost is for better test accessibility and increase the user population. All those decisions were made to develop an attractive test that would answer the technical expectations of healthcare professional.
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                                The <strong>perception</strong> of the test by the user was also analyzed to establish the preliminary basis of <strong>acceptability issues</strong>. The human relationship of the user during the test realization and the management of intimacy were our main reflection axis.
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                                Integration of the <strong>risks</strong> was also necessary. We identified 3 major risks. The contamination risk implies to use safe biological materials. The reliability deals with false positive and false negative. It is a technical issue that is related to our technology. The goal is to reduce them as much as possible. The use decay risk introduced by the user.
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                                The risk principle results in the concept of <strong>responsibility</strong>. <strong>Support</strong> is good way to reduce those risks, being supervised by the medical staff can be very reassuring during the test execution. The announcement of the results also questions the presence of medical staff according to the serious consequences of some STIs. The easy access and the simplicity of use are necessary to guarantee <strong>an equal right</strong> to get diagnosed.
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                                </br>Nowadays, the responsibility is not restricted to the user, it must also respect the <strong>environment</strong>. That’s what motivated our technical choices for the device.
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                                The matrix was also intended to be <srong>a decision helper tool</srong>. It was very useful when taking important decision (e.g. changing the revelation system).
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                                </br>Matching technical solutions to ethical values (accessibility, respect of the user, biosafety) can be really complex but stimulating!
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                                <br/><span style="text-indent:2em" class="glyphicon glyphicon-chevron-right"></span> <strong>Sample choices</strong>: blood for a non invasive test and easily standardized sample.
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                                <br/><span style="text-indent:2em" class="glyphicon glyphicon-chevron-right"></span> <strong>Materials choices</strong>: paper, biosourced plastic…for an eco friendly cycle of life.
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                                <br/><span style="text-indent:2em" class="glyphicon glyphicon-chevron-right"></span> <strong>Early-detection</strong>: RT instead of the p24 to detect the early stage infection of HIV.
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                                <br/><span style="text-indent:2em" class="glyphicon glyphicon-chevron-right"></span> <strong>Reliability</strong>: a system that minimizes the false negative risk by using two epitopes instead of one.
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                                <br/><span style="text-indent:2em" class="glyphicon glyphicon-chevron-right"></span> <strong>Usability</strong>: redaction of a usernote to improve the user experience.
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                                <br/><span style="text-indent:2em" class="glyphicon glyphicon-chevron-right"></span> <strong>Biosafety</strong>: latex beads instead of RT proteins or HbSAg as bio-safe migration controls.
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                                This work can serve as an inspiring example to other 2016 iGEM teams: Stanford-Brown and Michigan. We could organize an ethical debate about our technology. Our work on an ethical matrix is documented but there are still a lot of questions (writen in red in the matrix). With others, we can build upon to answer to doubt.
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                                </br>We were able to come up with a device that combines the expertise of physicians specializing in STIs, potential patients’ expectations, curiosity and foresight of young engineering students. So this device can be usable and useful.
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Arsenic is a toxic chemical that plagues populations around the world. Due to the direct relationship between arsenic contamination and a country’s disease burden, arsenic infiltration into soil and groundwater poses a serious threat to international productivity. Unfortunately, arsenic contamination in both developing and developed countries continues to beleaguer populations with health concerns due to the expensive and time-consuming nature of arsenic testing.</span></span></span></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">These problems are especially relevant to soldiers, who are often deployed in resource-scarce field environments. The Cadets2Vets team has harnessed the principles of synthetic biology to develop an inexpensive, portable means of arsenic detection. The biological portion of our arsenic sensor is made up of </span><span style="font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"> plasmid DNA. Encoded in this genetic material is an arsenic regulatory protein (ArsR) that triggers expression of a green fluorescent protein (GFP) in the presence of arsenic ions.</span></span></span></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Our construct is designed to function on a paper-based ticket built by the Edgewood Chemical and Biological Center (ECBC). Additionally, the Cadets2Vets team and ECBC have created a low-cost camera prototype for quantitative assessment of arsenic in our tickets. The ultimate goal of this project is to distribute our arsenic sensor to populations that are struggling with environmental contamination and its medical repercussions, as well as provide a tool to the United States military for detection of harmful substances.</span></span></p>
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Latest revision as of 00:11, 2 November 2017

HOME
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JUDGING FORM

Cadets2Vets


The Issue






As outlined by measure 7061A from the Environmental Protection Agency, atomic absorption/gaseous hydride is the current method for arsenic detection in wastes, soils, and groundwater. The method begins with a nitric or sulfuric acid digest. Using tin chloride, the arsenic is reduced into its trivalent state and converted into a volatile hydride. The hydride is then swept into an argon-hydrogen flame.The subsequent radiation is read by a spectrophotometer; the result is proportional to the arsenic concentration in the environmental sample. This process requires highly trained technicians and a significant delay for laboratory proceedings to be completed, costing up to $150 per sample.


Evidently, the acid digest method for arsenic detection is simply not reasonable for the impoverished people of Bangladesh, who face a severe crisis in arsenic contamination. It is estimated that nearly 57 million citizens of the Bengal basin are currently consuming water that exceeds the World Health Organization’s standard of arsenic concentration at ten parts per billion (Henke, 2009). WHO investigators estimate that more than 20% of deaths in Bangladesh could be attributed to dangerous concentrations of arsenic in tube wells. Additionally, the United States military remains concerned about exposure of troops to unsafe environmental conditions, including arsenic-contaminated drinking water. These issues show an urgent need for a field-effective, inexpensive sensor for arsenic.



Our Project






Arsenic is a toxic chemical that plagues populations around the world. Due to the direct relationship between arsenic contamination and a country’s disease burden, arsenic infiltration into soil and groundwater poses a serious threat to international productivity. Unfortunately, arsenic contamination in both developing and developed countries continues to beleaguer populations with health concerns due to the expensive and time-consuming nature of arsenic testing.


These problems are especially relevant to soldiers, who are often deployed in resource-scarce field environments. The Cadets2Vets team has harnessed the principles of synthetic biology to develop an inexpensive, portable means of arsenic detection. The biological portion of our arsenic sensor is made up of E. coli plasmid DNA. Encoded in this genetic material is an arsenic regulatory protein (ArsR) that triggers expression of a green fluorescent protein (GFP) in the presence of arsenic ions.


Our construct is designed to function on a paper-based ticket built by the Edgewood Chemical and Biological Center (ECBC). Additionally, the Cadets2Vets team and ECBC have created a low-cost camera prototype for quantitative assessment of arsenic in our tickets. The ultimate goal of this project is to distribute our arsenic sensor to populations that are struggling with environmental contamination and its medical repercussions, as well as provide a tool to the United States military for detection of harmful substances.



"Cadets2Vets is a team that unites military and civilian resources to investigate new ways of detecting toxic arsenic contamination in the environment. Our team is a community lab made up of students from the U.S. Military Academy at West Point, high school, and undergraduate students from schools in the Puget Sound area. We are using synthetic biology to develop a gene circuit that detects arsenic ions and then produces a reporter protein in response. The arsenic sensor can be used in military or civilian applications to help inform people of the possible dangers they may encounter while out in the field or at home."

- PI Dr. David Hirschberg, iGEM 2017


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2304 Jefferson Ave, Tacoma, WA 98402