Difference between revisions of "Team:Cadets2Vets"

 
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<head><meta name="viewport" content="width=device-width, initial-scale=1"><meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><script>function disputeIframe(){setTimeout(function (){self.document.open();self.document.write("<h1><a style='font-weight: normal; color: #000; font-family: arial;' href='" + self.location.href + "' target='_top'>Iframes are not supported on a free plan.</a></h1>");                    self.document.close();},200);} try {if (self!=top){disputeIframe();}}catch(e){disputeIframe();}</script><link href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/bootstrap3.min.css" rel="stylesheet"><link rel="stylesheet" href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/font-awesome.min4.css"><link href="//d33rxv6e3thba6.cloudfront.net/asset/sites/css/hover-min.css" rel="stylesheet"><link href="/static/css/style.css?v=2.0.4" rel="stylesheet"><link href="/static/css/blocks.css?v=2.0.4" rel="stylesheet"><link href="/static/css/theme-css/ez.css?v=2.0.4" rel="stylesheet"><style  type='text/css' data='Page Custom CSS'></style><script src="//code.jquery.com/jquery-1.12.4.min.js" integrity="sha256-ZosEbRLbNQzLpnKIkEdrPv7lOy9C27hHQ+Xp8a4MxAQ=" crossorigin="anonymous"></script><script> if (!window.jQuery) {  document.write('<script src="//d33rxv6e3thba6.cloudfront.net/asset/sites/js/jquery.js"></scr' + 'ipt>'); }</script><meta name="page-source" site="//sites.simbla.com/52b5a023-1bcf-d512-a412-3351ef830f04" page="home" site-id="593aca5737cad4ed7fa1fc16" page-id="593aca7437cad4ed7fa1fc1a" master-page-id="" /><link rel="canonical" href="//sites.simbla.com/52b5a023-1bcf-d512-a412-3351ef830f04/" /></head><body data-spy="scroll" data-target=".navbar-collapse" class="static-page"><div id="windowDiv"><div id="MarginsTop" style="height: 0px;" class="row"></div><div id="bodyContainer" style="width:100%">
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<div class="simblaEL containerHolder" data-drag="P133" id="P133" style=""><a class="simblaEL anchorElm" data-drag="divCounter" name="Intro0" id="Intro0"></a><div class="container-fluid minHeight100 active secondary" data-border-type="All" style="min-height: 694px; background-position: initial; background-size: cover; background-repeat: no-repeat; background-attachment: initial; background-origin: initial; background-clip: initial; background-image: url(&quot;https://s3.amazonaws.com/simbla-static/2017/5/593aca5737cad4ed7fa1fc16/32749-1eib9hn.jpeg&quot;); border-radius: 0px;"><div class="simblaEL containerHolder" data-drag="P135" id="P135"><div class="container" style=""><header class="simblaEL container-fluid sticky shadow-low" data-drag="P137" id="P137" data-slide-type="fade" data-opacity="95" data-style-class="basic" style="position: relative; padding-top: 30px;"><div class="row simblaEL rDivider" data-drag="P139" id="P139"><div class="sDivider col-md-3 index0" data-colsize="3"></div><div class="sDivider col-md-9 index1" data-colsize="9"></div></div></header><div class="row simblaEL rDivider" data-drag="P145" id="P145" style="position: relative; padding-top: 180px;"><div class="sDivider col-md-12 index0" data-colsize="12"></div></div><div class="row simblaEL rDivider" data-drag="P147" id="P147" style="position: relative; padding-top: 30px;"><div class="sDivider col-md-5 index0" data-colsize="5"></div><div class="sDivider col-md-2 index1" data-colsize="2"></div><div class="sDivider col-md-5 index2" data-colsize="5"><div class="simblaEL tcH" data-drag="P149" id="P149" style="position: relative;"><div class="textContainer H1" style="cursor: text; border-radius: 0px; padding: 10px 15px; background-color: rgb(255, 255, 255);" data-border-type="All"><h1><div style="text-align: center;"><font color="#444444">Welcome to the Cadets2Vets iGEM Webpage</font></div></h1></div></div></div></div></div></div></div></div><div class="simblaEL containerHolder" data-drag="P100" id="P100" style="position: relative;"><a class="simblaEL anchorElm" data-drag="divCounter" name="Footer0" id="Footer0"></a><div class="container-fluid active secondary" style="padding-bottom: 30px;"><div class="simblaEL containerHolder" data-drag="P102" id="P102" style="position: relative;"><div class="container" style="padding-top: 50px; 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<span id="element-09ca318ecaf1b2f"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook July</span>
 +
</a>
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</li>
  
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    </div>
+
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<span id="element-367041e6445b247"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook June</span>
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</a>
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 +
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<a class="removable-parent" href="public-engagement.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-307cb61a9a844e1"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
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<span id="element-fa38138e1dd4fce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
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</a>
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<div class="column full_size" >
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<img src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
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<span id="element-8308751370782bc"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
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<a class="removable-parent" href="basic-parts.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-78fbd0423085ce2"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
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<a class="removable-parent" href="composite-parts.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-24ea54f35798e9a"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Composite Parts</span>
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<span id="element-8d68d4dbac38a10"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
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<span id="element-c92600692c09125"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Interlab</span>
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<a class="removable-parent" href="our-partners.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-be35f8069be1925"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Our Partners</span>
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<a class="removable-parent" href="notebook-september.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-4d7dd9f5c90ef9b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook September</span>
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<a class="removable-parent" href="environmental-project.html" data-link-type="EXISTING"  target="_self" >
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<span id="2d522622"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Environmental Project</span>
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<a class="removable-parent" href="hardware.html" data-link-type="EXISTING"  target="_self" >
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<span id="8d3cfa8c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Hardware</span>
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<a class="removable-parent" href="demonstrate.html" data-link-type="EXISTING"  target="_self" >
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<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
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</a>
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<li class="removable-parent">
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<a class="removable-parent" href="improve.html" data-link-type="EXISTING"  target="_self" >
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<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
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</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="results.html" data-link-type="EXISTING"  target="_self" >
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<span id="7e0ed437"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Results</span>
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</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="attribution.html" data-link-type="EXISTING"  target="_self" >
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<span id="8a69633b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Attribution</span>
 +
</a>
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</li>
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<li class="removable-parent">
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<a class="removable-parent" href="notebook-october.html" data-link-type="EXISTING"  target="_self" >
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<span id="643d5085"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span>
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</a>
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<a class="removable-parent" href="silver-hp.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-563894f97a8ebaf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Silver HP</span>
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</a>
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<li class="removable-parent">
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<a class="removable-parent" href="integrated-and-gold.html" data-link-type="EXISTING"  target="_self" >
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<span id="element-874635e92416b44"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span>
 +
</a>
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</li>
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<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
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</ul>
 
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<div class="layout-settings" style="display:none;" data-type="menu"
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data-MENU_OVERLAY="relative"
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data-MENU_SCROLL="false"
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data-ALWAYS_MINIFIED="false"
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data-MENU_POSITION="none"
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data-MENU_ALIGN="left"
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data-BACKGROUND_COLOR="rgb(124, 100, 41)"
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></div>
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</div>
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</div>
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</div>
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<!-- MENUS END -->
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<!-- FORM START -->
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<div  id="vbid-59a5b-tjgooy1n"  class="master item-box  page-box style-59a5b-ti3xte4k    button-effects btn_hover3      " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-ti3xte4k" data-preview-styleid='style-59a5b-ti3xte4k' data-preset-type-id="FORM">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
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<div class="page-wrapper item-wrapper form-wrapper ">
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-tjgooy1n" data-preview-style="style-59a5b-ti3xte4k" data-style="style-e4cfb-6huavktiba" data-orig-thumb-height="2416" data-orig-thumb-width="3500" data-vbid="vbid-59a5b-tjgooy1n" data-bgimg="https://static.igem.org/mediawiki/2017/7/7c/Army_in_Parade_Dress.jpeg">
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<div  class="multi-container preview image-cover" >
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<div class="Picture item-preview">
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<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
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</div>
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<div class="helper-div middle-center" >
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<!-- <div class="benet" style="min-height:inherit;"></div> -->
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<div class="pic-side">
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<div class="vertical-aligner">
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<div id="vbid-59a5b-nnn4mbrq-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
 +
<div  id="vbid-59a5b-nnn4mbrq" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/7/7c/Army_in_Parade_Dress.jpeg);"  data-orig-width="3500" data-orig-height="2416" >
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<!-- VIDEO TEMPLATE -->
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 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
−
<div class="column full_size" >
 
−
<h1> Welcome to iGEM 2017! </h1>
 
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
 
−
</div>
 
  
−
<div class="clear"></div>
+
<!-- MAP TEMPLATE -->
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<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
−
<div class="column half_size" >
+
<!-- RAW TEMPLATE -->
−
<h5>Before you start: </h5>
+
−
<p> Please read the following pages:</p>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<ul>
+
 
−
<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
+
</div>
−
<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
−
<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
+
−
</ul>
+
 
</div>
 
</div>
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<div class="text-side shrinker-parent">
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<div class="vertical-aligner">
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<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
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<div class="preview-content-holder shrinker-content">
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<!--  BY SPECIFIC ORDER -->
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<div class="preview-icon-holder Icon removable-parent order-handle" >
 +
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<img id="element-2e144876c8f0e64" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://static.igem.org/mediawiki/2017/thumb/d/d2/Cadets2Vets_Logo.jpg/800px-Cadets2Vets_Logo.jpg.png" />
 +
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</div>
  
−
<div class="column half_size" >
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<div class="highlight">
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−
<h5> Styling your wiki </h5>
+
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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</div>
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</div>
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</div>
 
</div>
 
</div>
 
</div>
 
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
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</div>
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</div>
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<!-- FORM END -->
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<!-- MAPS START -->
 +
<div  id="vbid-59a5b-qihkumq9"  class="master item-box  gallery-box style-59a5b-jqfr7efo    button-effects btn_hover3      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-59a5b-jqfr7efo" data-preview-styleid='style-59a5b-jqfr7efo' data-preset-type-id="MAPS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
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<div class="gallery-wrapper item-wrapper maps-wrapper ">
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<div  class="sub container style-59a5b-jqfr7efo content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-59a5b-jqfr7efo" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-59a5b-qihkumq9" data-preset-type-id="MAPS" data-preview-style="style-59a5b-jqfr7efo" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
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data-ARRANGER_ITEM_RATIO="1"
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data-ARRANGER_ITEM_SPACING="20"
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data-ARRANGER_ITEMS_PER_PAGE="all"
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data-ARRANGER_ORDER_TYPE="regular"
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data-AUTO_PLAY="AUTOPLAY"
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data-AUTO_PLAY_DURATION="4"
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data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
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<div class="layout-settings" style="display:none;" data-type="multi"></div>
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<div id="children">
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<div id="items-holder-wrapper">
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<div id="items-holder">
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<div  id="vbid-59a5b-ghvxgbnu"  class="sub item-box  page-box style-59a5b-jqfr7efo          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-jqfr7efo" data-preview-styleid='style-59a5b-jqfr7efo' data-preset-type-id="UNRESOLVED">
 +
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<div class="page-wrapper item-wrapper ">
 +
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-59a5b-ghvxgbnu" data-preview-style="style-59a5b-jqfr7efo" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-ghvxgbnu" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
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<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div class="column full_size" >
+
<div id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<h5> Wiki template information </h5>
+
<!-- VIDEO TEMPLATE -->
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
  
−
</div>  
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
  
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
−
<div class="column half_size" >
+
</div>
−
<h5> Editing your wiki </h5>
+
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
+
</div>
−
<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<div class="preview-icon-holder Icon removable-parent order-handle" >
 +
 +
<img id="vbid-59a5b-qgicu05p" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://static.igem.org/mediawiki/2017/3/30/US_Army_Logo.jpg" />
 +
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-59a5b-j6hw2jzr" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >WE ARE Cadets2Vets.</h2>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="vbid-59a5b-potfnoot" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, <br>Tacoma, WA 98402<br></h3>
  
−
<div class="column half_size" >
 
−
<h5>Tips</h5>
 
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
−
<ul>
 
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
−
<li>Be clear about what you are doing and how you plan to do this.</li>
 
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
 
−
<li>Have lots of fun! </li>
 
−
</ul>
 
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
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<span id="vbid-59a5b-hgd5ta77" class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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<p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><br></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span id="docs-internal-guid-07d9d787-1726-69e0-e827-dac88147f21b"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">As outlined by measure 7061A from the </span><span style="font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">Environmental Protection Agency</span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">, atomic absorption/gaseous hydride is the current method for arsenic detection in wastes, soils, and groundwater. The method begins with a nitric or sulfuric acid digest. Using tin chloride, the arsenic is reduced into its trivalent state and converted into a volatile hydride. The hydride is then swept into an argon-hydrogen flame.The subsequent radiation is read by a spectrophotometer; the result is proportional to the arsenic concentration in the environmental sample. This process requires highly trained technicians and a significant delay for laboratory proceedings to be completed, costing up to $150 per sample. </span></span></span></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><br></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span id="docs-internal-guid-07d9d787-1726-69e0-e827-dac88147f21b"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Evidently, the acid digest method for arsenic detection is simply not reasonable for the impoverished people of Bangladesh, who face a severe crisis in arsenic contamination. It is estimated that nearly 57 million citizens of the Bengal basin are currently consuming water that exceeds the World Health Organization’s standard of arsenic concentration at ten parts per billion (Henke, 2009). WHO investigators estimate that more than 20% of deaths in Bangladesh could be attributed to dangerous concentrations of arsenic in tube wells. Additionally, the United States military remains concerned about exposure of troops to unsafe environmental conditions,
 +
including arsenic-contaminated drinking water. These issues show an urgent need for a field-effective,
 +
inexpensive sensor for arsenic.</span></span></span></p>
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            <span dir="auto">Team:INSA-Lyon/Integrated Practices</span>
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<p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;background-color:#ffffff;"><span style="line-height:1em;"><span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"></span></span></span><span style="line-height:1em;"><span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"></span></span></span>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Arsenic is a toxic chemical that plagues populations around the world. Due to the direct relationship between arsenic contamination and a country’s disease burden, arsenic infiltration into soil and groundwater poses a serious threat to international productivity. Unfortunately, arsenic contamination in both developing and developed countries continues to beleaguer populations with health concerns due to the expensive and time-consuming nature of arsenic testing.</span></span></span></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">These problems are especially relevant to soldiers, who are often deployed in resource-scarce field environments. The Cadets2Vets team has harnessed the principles of synthetic biology to develop an inexpensive, portable means of arsenic detection. The biological portion of our arsenic sensor is made up of </span><span style="font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"> plasmid DNA. Encoded in this genetic material is an arsenic regulatory protein (ArsR) that triggers expression of a green fluorescent protein (GFP) in the presence of arsenic ions.</span></span></span></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Our construct is designed to function on a paper-based ticket built by the Edgewood Chemical and Biological Center (ECBC). Additionally, the Cadets2Vets team and ECBC have created a low-cost camera prototype for quantitative assessment of arsenic in our tickets. The ultimate goal of this project is to distribute our arsenic sensor to populations that are struggling with environmental contamination and its medical repercussions, as well as provide a tool to the United States military for detection of harmful substances.</span></span></p>
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Latest revision as of 00:11, 2 November 2017

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Cadets2Vets


The Issue






As outlined by measure 7061A from the Environmental Protection Agency, atomic absorption/gaseous hydride is the current method for arsenic detection in wastes, soils, and groundwater. The method begins with a nitric or sulfuric acid digest. Using tin chloride, the arsenic is reduced into its trivalent state and converted into a volatile hydride. The hydride is then swept into an argon-hydrogen flame.The subsequent radiation is read by a spectrophotometer; the result is proportional to the arsenic concentration in the environmental sample. This process requires highly trained technicians and a significant delay for laboratory proceedings to be completed, costing up to $150 per sample.


Evidently, the acid digest method for arsenic detection is simply not reasonable for the impoverished people of Bangladesh, who face a severe crisis in arsenic contamination. It is estimated that nearly 57 million citizens of the Bengal basin are currently consuming water that exceeds the World Health Organization’s standard of arsenic concentration at ten parts per billion (Henke, 2009). WHO investigators estimate that more than 20% of deaths in Bangladesh could be attributed to dangerous concentrations of arsenic in tube wells. Additionally, the United States military remains concerned about exposure of troops to unsafe environmental conditions, including arsenic-contaminated drinking water. These issues show an urgent need for a field-effective, inexpensive sensor for arsenic.



Our Project






Arsenic is a toxic chemical that plagues populations around the world. Due to the direct relationship between arsenic contamination and a country’s disease burden, arsenic infiltration into soil and groundwater poses a serious threat to international productivity. Unfortunately, arsenic contamination in both developing and developed countries continues to beleaguer populations with health concerns due to the expensive and time-consuming nature of arsenic testing.


These problems are especially relevant to soldiers, who are often deployed in resource-scarce field environments. The Cadets2Vets team has harnessed the principles of synthetic biology to develop an inexpensive, portable means of arsenic detection. The biological portion of our arsenic sensor is made up of E. coli plasmid DNA. Encoded in this genetic material is an arsenic regulatory protein (ArsR) that triggers expression of a green fluorescent protein (GFP) in the presence of arsenic ions.


Our construct is designed to function on a paper-based ticket built by the Edgewood Chemical and Biological Center (ECBC). Additionally, the Cadets2Vets team and ECBC have created a low-cost camera prototype for quantitative assessment of arsenic in our tickets. The ultimate goal of this project is to distribute our arsenic sensor to populations that are struggling with environmental contamination and its medical repercussions, as well as provide a tool to the United States military for detection of harmful substances.



"Cadets2Vets is a team that unites military and civilian resources to investigate new ways of detecting toxic arsenic contamination in the environment. Our team is a community lab made up of students from the U.S. Military Academy at West Point, high school, and undergraduate students from schools in the Puget Sound area. We are using synthetic biology to develop a gene circuit that detects arsenic ions and then produces a reporter protein in response. The arsenic sensor can be used in military or civilian applications to help inform people of the possible dangers they may encounter while out in the field or at home."

- PI Dr. David Hirschberg, iGEM 2017


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2304 Jefferson Ave, Tacoma, WA 98402