Difference between revisions of "Team:Cadets2Vets"

 
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<title>Cadets2Vets</title>
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<meta charset="UTF-8">
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<meta name="viewport" content="width=device-width, maximum-scale=1"/>
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<meta name="keywords" content="Cadets2Vets IGEM igem RAIN ">
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<meta name="description" content="We are a community biological lab competing in IGEM">
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<link rel="icon" type="image/png" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
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<link rel="apple-touch-icon" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
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</script></head><body id="editor" class="editorMode"><div id="windowDiv"><div id="MarginsTop" style="height: 0px;" class="row"></div><div id="bodyContainer" style="width:100%"></div></div></body>
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</div>             <div class="visualClear"></div>
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                <link id="vbid-59a5b-fnxcttvm-STATIC_STYLE" href="https://2017.igem.org/Team:Cadets2Vets/css/static_style?action=raw&ctype=text/css" rel="stylesheet">
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            </div>
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    </div>
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<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/xprs_helper?action=raw&ctype=text/javascript"></script>
  
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<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/all_js?action=raw&ctype=text/javascript"></script>
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UPLOAD FILES
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                        <p>Designed by Team iGEM Cadets2Vets </p>
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<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1" data-app-version="1.4.1f" data-caller="live">
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            <span dir="auto">Team:Cadets2Vets/Integrated Practices</span>
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<div id="content">
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<div id="children">
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HOME
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<span id="element-a1a56421418f7ce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 
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<span id="vbid-59a5b-hgd5ta77"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
  
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<p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><br></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span id="docs-internal-guid-07d9d787-1726-69e0-e827-dac88147f21b"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">As outlined by measure 7061A from the </span><span style="font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">Environmental Protection Agency</span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">, atomic absorption/gaseous hydride is the current method for arsenic detection in wastes, soils, and groundwater. The method begins with a nitric or sulfuric acid digest. Using tin chloride, the arsenic is reduced into its trivalent state and converted into a volatile hydride. The hydride is then swept into an argon-hydrogen flame.The subsequent radiation is read by a spectrophotometer; the result is proportional to the arsenic concentration in the environmental sample. This process requires highly trained technicians and a significant delay for laboratory proceedings to be completed, costing up to $150 per sample. </span></span></span></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><br></p><p dir="ltr" style="margin-top: 0pt; margin-bottom: 0pt; letter-spacing: 1.4px; line-height: 1.38; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span id="docs-internal-guid-07d9d787-1726-69e0-e827-dac88147f21b"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Evidently, the acid digest method for arsenic detection is simply not reasonable for the impoverished people of Bangladesh, who face a severe crisis in arsenic contamination. It is estimated that nearly 57 million citizens of the Bengal basin are currently consuming water that exceeds the World Health Organization’s standard of arsenic concentration at ten parts per billion (Henke, 2009). WHO investigators estimate that more than 20% of deaths in Bangladesh could be attributed to dangerous concentrations of arsenic in tube wells. Additionally, the United States military remains concerned about exposure of troops to unsafe environmental conditions,
 +
including arsenic-contaminated drinking water. These issues show an urgent need for a field-effective,
 +
inexpensive sensor for arsenic.</span></span></span></p>
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<p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;background-color:#ffffff;"><span style="line-height:1em;"><span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"></span></span></span><span style="line-height:1em;"><span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"></span></span></span>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Arsenic is a toxic chemical that plagues populations around the world. Due to the direct relationship between arsenic contamination and a country’s disease burden, arsenic infiltration into soil and groundwater poses a serious threat to international productivity. Unfortunately, arsenic contamination in both developing and developed countries continues to beleaguer populations with health concerns due to the expensive and time-consuming nature of arsenic testing.</span></span></span></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><br></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">These problems are especially relevant to soldiers, who are often deployed in resource-scarce field environments. The Cadets2Vets team has harnessed the principles of synthetic biology to develop an inexpensive, portable means of arsenic detection. The biological portion of our arsenic sensor is made up of </span><span style="font-size: 12pt; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;"> plasmid DNA. Encoded in this genetic material is an arsenic regulatory protein (ArsR) that triggers expression of a green fluorescent protein (GFP) in the presence of arsenic ions.</span></span></span></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><br></p>
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<p style="letter-spacing: 1.4px; background-color: rgb(255, 255, 255);"><span style="line-height: 1em;"><span style="font-size: 12pt; vertical-align: baseline; white-space: pre-wrap;">Our construct is designed to function on a paper-based ticket built by the Edgewood Chemical and Biological Center (ECBC). Additionally, the Cadets2Vets team and ECBC have created a low-cost camera prototype for quantitative assessment of arsenic in our tickets. The ultimate goal of this project is to distribute our arsenic sensor to populations that are struggling with environmental contamination and its medical repercussions, as well as provide a tool to the United States military for detection of harmful substances.</span></span></p>
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                  <a href="https://2017.igem.org/Team:Cadets2Vets/Bronze">Bronze</a>
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                  <a href="https://2017.igem.org/Team:Cadets2Vets/Gold">Gold</a>
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<div class="row pageBranding">EXPLORE NEPTUNE</div>
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<div class="row headerCaption">An end to end design suite for <br>
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continuous flow microfluidic devices </div>
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<a href="https://2017.igem.org/Team:Cadets2vets/Demonstrate" > <button type="button" class="btn btn-primary" id="down">GET STARTED</button></a>
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              <div class="col-md-6 noPadding"> SPECIFY & DESIGN </div>
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              <div class="col-md-6 noPadding"> FABRICATE </div>
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          <div class="col-md-4" style="font-size:15px">Begin by defining which inputs and outputs your microfluidic chip will have.
+
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Declare any limitations in terms of port size, channel width. Neptune will efficiently place and route the features of your chip.
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The mapper (MM) tool within the place and routing functionality has been made completely by the iGEM Team.
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          <div class="col-md-4" style="font-size:15px">Check and visualize your device layout using the Neptune graphical user interface.
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Edit the design if you so desire. Establish a control sequence to move fluids through the microfluidic chip.
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The interface has been made completely by the iGEM Team.
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          <div class="col-md-4" style="font-size:15px">Assemble your microfluidic system. Import our design files into a 3D Printer and CNC mill to make the chip and the pump setup.
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Change the setup using our parameterized designs to fit your specific needs.
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The design of the setup is BU iGEM work, whilst the chip manufacturing is the work of our mentors.
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<div style="text-align:center; font-size:31px;">OUR PROJECT</div>
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Neptune is a specify, design, and build tool for the development of continuous flow microfluidics. With Neptune, users can specify microfluidic devices through a high level description of liquid flow relations. Our application will automatically place and route the design schematic of the microfluidic and, when used in conjunction with low cost and readily available CAD tools, a user can build their custom microfluidic system in-lab.
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The Boston University 2016 iGEM Hardware Team is encapsulating this entire workflow through a graphical user interface. Users can write files that specify the function of the microfluidic, preview and edit their microfluidic design generated by the application, use this application directly with CAD tools such as a CNC mill to fabricate their device, and physically control valves and ports on the microfluidic chip. The iGEM team is also complementing this workflow by developing and releasing open source designs of our hardware, including parametric 3D print files and firmware for microcontrollers that actuate the microfluidic valves. Finally, the iGEM team is also involved in the development of the place and route software that converts a high level description of liquid flow relations into a microfluidic netlist that can that be converted into a design schematic.
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Latest revision as of 00:11, 2 November 2017

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Cadets2Vets


The Issue






As outlined by measure 7061A from the Environmental Protection Agency, atomic absorption/gaseous hydride is the current method for arsenic detection in wastes, soils, and groundwater. The method begins with a nitric or sulfuric acid digest. Using tin chloride, the arsenic is reduced into its trivalent state and converted into a volatile hydride. The hydride is then swept into an argon-hydrogen flame.The subsequent radiation is read by a spectrophotometer; the result is proportional to the arsenic concentration in the environmental sample. This process requires highly trained technicians and a significant delay for laboratory proceedings to be completed, costing up to $150 per sample.


Evidently, the acid digest method for arsenic detection is simply not reasonable for the impoverished people of Bangladesh, who face a severe crisis in arsenic contamination. It is estimated that nearly 57 million citizens of the Bengal basin are currently consuming water that exceeds the World Health Organization’s standard of arsenic concentration at ten parts per billion (Henke, 2009). WHO investigators estimate that more than 20% of deaths in Bangladesh could be attributed to dangerous concentrations of arsenic in tube wells. Additionally, the United States military remains concerned about exposure of troops to unsafe environmental conditions, including arsenic-contaminated drinking water. These issues show an urgent need for a field-effective, inexpensive sensor for arsenic.



Our Project






Arsenic is a toxic chemical that plagues populations around the world. Due to the direct relationship between arsenic contamination and a country’s disease burden, arsenic infiltration into soil and groundwater poses a serious threat to international productivity. Unfortunately, arsenic contamination in both developing and developed countries continues to beleaguer populations with health concerns due to the expensive and time-consuming nature of arsenic testing.


These problems are especially relevant to soldiers, who are often deployed in resource-scarce field environments. The Cadets2Vets team has harnessed the principles of synthetic biology to develop an inexpensive, portable means of arsenic detection. The biological portion of our arsenic sensor is made up of E. coli plasmid DNA. Encoded in this genetic material is an arsenic regulatory protein (ArsR) that triggers expression of a green fluorescent protein (GFP) in the presence of arsenic ions.


Our construct is designed to function on a paper-based ticket built by the Edgewood Chemical and Biological Center (ECBC). Additionally, the Cadets2Vets team and ECBC have created a low-cost camera prototype for quantitative assessment of arsenic in our tickets. The ultimate goal of this project is to distribute our arsenic sensor to populations that are struggling with environmental contamination and its medical repercussions, as well as provide a tool to the United States military for detection of harmful substances.



"Cadets2Vets is a team that unites military and civilian resources to investigate new ways of detecting toxic arsenic contamination in the environment. Our team is a community lab made up of students from the U.S. Military Academy at West Point, high school, and undergraduate students from schools in the Puget Sound area. We are using synthetic biology to develop a gene circuit that detects arsenic ions and then produces a reporter protein in response. The arsenic sensor can be used in military or civilian applications to help inform people of the possible dangers they may encounter while out in the field or at home."

- PI Dr. David Hirschberg, iGEM 2017


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