Difference between revisions of "Team:TCFSH Taiwan/Composite Part"

 
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<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.1.0/jquery.min.js"></script>
 
<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.1.0/jquery.min.js"></script>
 
<link href="https://2016.igem.org/Team:NCTU_Formosa/wiki_reset?action=raw&ctype=text/css" rel="stylesheet">
 
 
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400" rel="stylesheet">
 
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400" rel="stylesheet">
  
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   width: 16px;
 
   width: 16px;
 
   height: 16px;
 
   height: 16px;
   background:#00D6FF;/*active circle color */
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   background:red;/*active circle color */
 
   border-radius: 100%;
 
   border-radius: 100%;
 
   position: absolute;
 
   position: absolute;
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     padding-bottom:15px;
 
     padding-bottom:15px;
 
}
 
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/*second title*/
 
/*second title*/
 
.content-1{
 
.content-1{
     font-size:20pt !important;
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     font-size:10pt !important;
 
     text-align:justify;
 
     text-align:justify;
     color:#44E287;
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    text-indent:2em;
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     color:#ff9933;
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     padding-top:5px;
 
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     padding-bottom:10px
 
     padding-bottom:10px
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}
 
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.bigphoto{
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<!--compositepart 1-->
 
<!--compositepart 1-->
<a href="http://parts.igem.org/Part:BBa_K2383100" class="biobrick"><p class="title">Composite BBa_K2383100</p>
+
<a href="http://parts.igem.org/Part:BBa_K2383100" class="biobrick"><p class="title">Composite BBa_K2383100</p></a>
 
<p class="content"> We us the cjBlue_LVA in this part, and add UirR, UirS into it. The biobrick will be like:</p>
 
<p class="content"> We us the cjBlue_LVA in this part, and add UirR, UirS into it. The biobrick will be like:</p>
  
<img src="https://static.igem.org/mediawiki/2017/a/ad/Compositepart1.png" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/1/17/Brick4.jpeg" class="bigphoto" width=70%>
  
<p class="content">R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)</p>
+
<p class="content-1">R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)</p>
  
 
<!--compositepart 2-->
 
<!--compositepart 2-->
  
<a href="http://parts.igem.org/Part:BBa_K2383200" class="biobrick"><p class="title">Composite BBa_K2383200</p>
+
<a href="http://parts.igem.org/Part:BBa_K2383200" class="biobrick"><p class="title">Composite BBa_K2383200</p></a>
 
<p class="content">This is a RhlR Regulating system with RNA thermometer. The biobrick will be like:</p>
 
<p class="content">This is a RhlR Regulating system with RNA thermometer. The biobrick will be like:</p>
  
<img src="https://static.igem.org/mediawiki/2017/a/ad/Compositepart1.png" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/9/9e/Brick3.jpeg" class="bigphoto" width=70%>
  
<p class="content">J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 </p>
+
<p class="content-1">J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 (RhlI)</p>
  
 
<!--compositepart 3-->
 
<!--compositepart 3-->
  
<a href="http://parts.igem.org/Part:BBa_K2383300" class="biobrick"><p class="title">Composite BBa_K2383300</p>
+
<a href="http://parts.igem.org/Part:BBa_K2383300" class="biobrick"><p class="title">Composite BBa_K2383300</p></a>
 
<p class="content">This is a Report system of UV and Temperature with LacI. The biobrick will be like:</p>
 
<p class="content">This is a Report system of UV and Temperature with LacI. The biobrick will be like:</p>
  
<img src="https://static.igem.org/mediawiki/2017/a/ad/Compositepart1.png" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/4/4c/Brick6.jpeg" class="bigphoto" width=70%>
 +
 
 +
<p class="content-1">B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)</p>
 +
 
 +
<!--compositepart 4-->
 +
 
 +
<a href="http://parts.igem.org/Part:BBa_K2383301" class="biobrick"><p class="title">Composite BBa_K2383301</p></a>
 +
<p class="content">This is a Report system of UV with LacI. The biobrick will be like:</p>
  
<p class="content">B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)</p>
+
<img src="https://static.igem.org/mediawiki/2017/7/7a/Brick2.jpeg" class="bigphoto" width=70%>
 +
<p class="content-1">BBa_K1725400 (PlsiR) + B0034 + E1010 (RFP) + B0034 + BBa_K2383010</p>
  
 +
<!--compositepart 5-->
  
 +
<a href="http://parts.igem.org/Part:BBa_K2383302" class="biobrick"><p class="title">Composite BBa_K2383302</p></a>
 +
<p class="content">This is a Report system of Temperature with LacI. The biobrick will be like:</p>
  
 +
<img src="https://static.igem.org/mediawiki/2017/0/0c/Brick5.jpeg" class="bigphoto" width=70%>
 +
<p class="content-1">BBa_K1725400 (PlsiR) + B0034 + BBa_K592009 + B0034 + BBa_K2383010 (LacI) + B0010 + B0012</p>
  
 +
<p class="content">These are the composite parts we use in our project.</p>
  
 
</div>
 
</div>

Latest revision as of 01:04, 2 November 2017

Composite Parts

We have know the function of two basic parts, the cjBlue-LVA and LacI without LVA. Hence, we create biobrick using the basic parts we mentioned and other different parts which will be mentioned below.

Composite BBa_K2383100

We us the cjBlue_LVA in this part, and add UirR, UirS into it. The biobrick will be like:

R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)

Composite BBa_K2383200

This is a RhlR Regulating system with RNA thermometer. The biobrick will be like:

J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 (RhlI)

Composite BBa_K2383300

This is a Report system of UV and Temperature with LacI. The biobrick will be like:

B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)

Composite BBa_K2383301

This is a Report system of UV with LacI. The biobrick will be like:

BBa_K1725400 (PlsiR) + B0034 + E1010 (RFP) + B0034 + BBa_K2383010

Composite BBa_K2383302

This is a Report system of Temperature with LacI. The biobrick will be like:

BBa_K1725400 (PlsiR) + B0034 + BBa_K592009 + B0034 + BBa_K2383010 (LacI) + B0010 + B0012

These are the composite parts we use in our project.