Difference between revisions of "Team:CMUQ/BioSensor Lab R"

 
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<div>
 
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<button type="button" class="btn btn-default btn-lg btn-block" style= "font-size: 35px;"><a href="https://2017.igem.org/Team:CMUQ/Results"> Results<a/></button>
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<button type="button" class="btn btn-default btn-lg btn-block" style= "font-size: 35px;"><a href="https://2017.igem.org/Team:CMUQ/Results"> Results</a></button>
 +
 
 +
<p style="font-size: 20px;margin-left:40px; margin-right40px;">As we were unable to transform the construct, therefore, the results for Salt Sensor testing were obtained from iGEM Pitt as a part of the collaboration </p>
 +
 
 +
 
 +
<p  style="font-size: 20px;margin-left:40px; margin-right40px;">1. The results for the RFP fluorescence measured for three replicated of the blank as well as two constructs at excitation 585nm/emission 607nm. </p>
 +
<table style="width:50%; margin-left:100px;">
 +
 
 +
  <tr>
 +
    <th>NFkB (negative control)</th>
 +
    <th>0mM</th>
 +
    <th>50mM</th>
 +
    <th>100mM</th>
 +
    <th>200mM</th>
 +
    <th>400mM</th>
 +
    <th>800mM</th>
 +
    <th>1600mM</th>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>1</td>
 +
    <td>326525</td>
 +
    <td>322701</td>
 +
    <td>324569</td>
 +
    <td>324359</td>
 +
    <td>329257</td>
 +
    <td>424215</td>
 +
    <td>447835</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>2</td>
 +
    <td>324213</td>
 +
    <td>321232</td>
 +
    <td>323679</td>
 +
   
 +
    <td>324437</td>
 +
    <td>326850</td>
 +
    <td>421070</td>
 +
    <td>436049</td>
 +
  </tr>
 +
 
 +
  <tr>
 +
        <td>3</td>
 +
    <td>321979</td>
 +
    <td>321615</td>
 +
    <td>322685</td>
 +
   
 +
    <td>324569</td>
 +
    <td>327046</td>
 +
    <td>399897</td>
 +
    <td>406696</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
 +
 
 +
  <tr>
 +
    <th>Wildtype</th>
 +
    <th>0mM</th>
 +
    <th>50mM</th>
 +
    <th>100mM</th>
 +
    <th>200mM</th>
 +
    <th>400mM</th>
 +
    <th>800mM</th>
 +
    <th>1600mM</th>
 +
  </tr>
 +
 
 +
  <tr>
 +
    <td>1</td>
 +
    <td>322379</td>
 +
    <td>324207</td>
 +
    <td>326546</td>
 +
    <td>324794</td>
 +
    <td>330924</td>
 +
    <td>341689</td>
 +
    <td>388728</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>2</td>
 +
    <td>322539</td>
 +
    <td>323331</td>
 +
    <td>326077</td>
 +
   
 +
    <td>324704</td>
 +
    <td>331310</td>
 +
    <td>367254</td>
 +
    <td>438918</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>3</td>
 +
    <td>322369</td>
 +
    <td>323430</td>
 +
    <td>326386</td>
 +
   
 +
    <td>324750</td>
 +
    <td>330914</td>
 +
    <td>353266</td>
 +
    <td>381339</td>
 +
 
 +
  </tr> 
 +
  <tr>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
 +
  <tr>
 +
    <th>Consensus</th>
 +
    <th>0mM</th>
 +
    <th>50mM</th>
 +
    <th>100mM</th>
 +
    <th>200mM</th>
 +
    <th>400mM</th>
 +
    <th>800mM</th>
 +
    <th>1600mM</th>
 +
  </tr>
 +
    <tr>
 +
    <td>1</td>
 +
    <td>326742</td>
 +
    <td>326016</td>
 +
    <td>325673</td>
 +
    <td>329309</td>
 +
    <td>337418</td>
 +
    <td>377574</td>
 +
    <td>378246</td>
 +
  </tr>
 +
    <tr>
 +
    <td>2</td>
 +
    <td>325087</td>
 +
    <td>326368</td>
 +
    <td>326416</td>
 +
    <td>327722</td>
 +
    <td>337804</td>
 +
    <td>367197</td>
 +
    <td>400206</td>
 +
  </tr>
 +
    <tr>
 +
    <td>3</td>
 +
    <td>325689</td>
 +
    <td>324992</td>
 +
    <td>325312</td>
 +
    <td>327109</td>
 +
    <td>334483</td>
 +
    <td>364028</td>
 +
    <td>373127</td>
 +
  </tr>
 +
 
 +
 
 +
</table>
 +
<p  style="font-size: 20px;margin-left:40px; margin-right40px;">2. Averaging and normalizing the fluorescence readings measured for our blank and two constructs. </p>
 +
 
 +
<table style="width:50%; margin-left:100px;">
 +
 
 +
  <tr>
 +
  <th></th>
 +
    <th></th>
 +
    <th>0mM</th>
 +
    <th>50mM</th>
 +
    <th>100mM</th>
 +
    <th>200mM</th>
 +
    <th>400mM</th>
 +
    <th>800mM</th>
 +
    <th>1600mM</th>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td rowspan="3">Average </td>
 +
    <td>NFkB</td>
 +
    <td>324239</td>
 +
    <td>321849.33</td>
 +
    <td>323644.33</td>
 +
    <td>324455</td>
 +
    <td>327717.7</td>
 +
    <td>415060.7</td>
 +
    <td>430193.3 </td>
 +
 
 +
  </tr>
 +
  <tr>
 +
   
 +
    <td>Consensus</td>
 +
    <td>325839.33</td>
 +
    <td>325792</td>
 +
    <td>325800.33</td>
 +
   
 +
    <td>328046.7</td>
 +
    <td>336568.3</td>
 +
    <td>369599.7</td>
 +
    <td>383859.7</td>
 +
  </tr>
 +
 
 +
  <tr>
 +
  <td>  Wildtype</td>
 +
        <td>322429</td>
 +
    <td>323656</td>
 +
    <td>326336.33</td>
 +
    <td>324749.3</td>
 +
 
 +
    <td>331049.3</td>
 +
    <td>354069.7</td>
 +
    <td>402995</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
    <td></td>
 +
  </tr>
 +
 
 +
  <tr>
 +
    <td rowspan="2">NFkB</td>
 +
    <th>Consensus</th>
 +
    <th>1600.3333</th>
 +
    <th>3942.6667</th>
 +
    <th>2156</th>
 +
    <th>3591.667</th>
 +
    <th>8850.667</th>
 +
    <th>-45461</th>
 +
    <th>-46333.7 </th>
 +
  </tr>
 +
 
 +
  <tr>
 +
    <td>WildType</td>
 +
    <td>-1810</td>
 +
    <td>1806.6667</td>
 +
    <td>2692</td>
 +
    <td>294.3333</td>
 +
    <td>3331.667</td>
 +
    <td>-60991</td>
 +
    <td>-27198.3</td>
 +
 
 +
  </tr>
 +
 
 +
 
 +
 
 +
</table>
 +
 
 +
<p  style="font-size: 20px;margin-left:40px; margin-right40px;">3. The normalized fluorescence values for the two constructs at different NaCl concentrations.
 +
 </p>
 +
<table style="width:50%; margin-left:100px;">
 +
 
 +
  <tr>
 +
 
 +
    <th>NaCl Concentration (mM)</th>
 +
    <th>Wildtype</th>
 +
    <th>Consensus</th>
 +
   
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>0 </td>
 +
    <td>-1810</td>
 +
    <td>1600.33</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
   
 +
    <td>50</td>
 +
    <td>1806.67</td>
 +
    <td>3942.67</td>
 +
  </tr>
 +
 
 +
  <tr>
 +
  <td>  100</td>
 +
  <td>2692</td>
 +
    <td>2156</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>200</td>
 +
    <td>294.33</td>
 +
    <td>3591.67</td>
 +
  </tr>
 +
 
 +
  <tr>
 +
    <td>400</td>
 +
    <td>3331.67</td>
 +
    <td>8850.67</td>
 +
  </tr>
 +
 
 +
  <tr>
 +
    <td>800</td>
 +
    <td>-60991</td>
 +
    <td>-45461</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>1600</td>
 +
    <td>-27198.33</td>
 +
    <td>-46333.67</td>
 +
 
 +
  </tr>
 +
 
 +
 
 +
 
 +
</table>
 +
 
 +
 
 +
 
  
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Latest revision as of 01:32, 2 November 2017

BioSensor Lab Results

As we were unable to transform the construct, therefore, the results for Salt Sensor testing were obtained from iGEM Pitt as a part of the collaboration

1. The results for the RFP fluorescence measured for three replicated of the blank as well as two constructs at excitation 585nm/emission 607nm. 

NFkB (negative control) 0mM 50mM 100mM 200mM 400mM 800mM 1600mM
1 326525 322701 324569 324359 329257 424215 447835
2 324213 321232 323679 324437 326850 421070 436049
3 321979 321615 322685 324569 327046 399897 406696
Wildtype 0mM 50mM 100mM 200mM 400mM 800mM 1600mM
1 322379 324207 326546 324794 330924 341689 388728
2 322539 323331 326077 324704 331310 367254 438918
3 322369 323430 326386 324750 330914 353266 381339
Consensus 0mM 50mM 100mM 200mM 400mM 800mM 1600mM
1 326742 326016 325673 329309 337418 377574 378246
2 325087 326368 326416 327722 337804 367197 400206
3 325689 324992 325312 327109 334483 364028 373127

2. Averaging and normalizing the fluorescence readings measured for our blank and two constructs. 

0mM 50mM 100mM 200mM 400mM 800mM 1600mM
Average NFkB 324239 321849.33 323644.33 324455 327717.7 415060.7 430193.3
Consensus 325839.33 325792 325800.33 328046.7 336568.3 369599.7 383859.7
Wildtype 322429 323656 326336.33 324749.3 331049.3 354069.7 402995
NFkB Consensus 1600.3333 3942.6667 2156 3591.667 8850.667 -45461 -46333.7
WildType -1810 1806.6667 2692 294.3333 3331.667 -60991 -27198.3

3. The normalized fluorescence values for the two constructs at different NaCl concentrations.  

NaCl Concentration (mM) Wildtype Consensus
0 -1810 1600.33
50 1806.67 3942.67
100 2692 2156
200 294.33 3591.67
400 3331.67 8850.67
800 -60991 -45461
1600 -27198.33 -46333.67