Latest revision as of 02:03, 2 November 2017

Dspb Lab Results

The following results were obtained for the Dispersin B, which is an improvement on an Existing part:

1. The results for the GFP fluorescence measured for three replicates of the LB (blank) as well as two constructs at excitation 585nm/emission 607nm

				Average	SD	t test
LB	-0.004	-0.0013	-0.055	-0.0201	0.030254421
t=0 supernatant	-0.034	0.009	0.005	0.006666667	0.023755701	0.328585241
t=16 supernatant	0.153	0.038	0.046	0.079	0.064210591	0.050130586

2. Averaging and normalizing the fluorescence readings measured from culture before pelleting the cells and then after pelleting the cells.

	Fluorescence
pDP12	-0.033	0.02	0.036	0.007666667	0.036115555
cells t=0	-0.029	0.008	-0.008	-0.009666667	0.018556221
cells t=16	0.09	0.032	0.07	0.064	0.02946184
supernatant t=0	0	-0.145	0.0065	-0.046166667	0.085653858
supernatant t=16	0.048	0.122	0.045	0.071666667	0.043615746

Norm. cells t=0	0.004	-0.012	-0.044	-0.017333333	0.024440404
Norm. cells t=16	0.123	0.012	0.034	0.056333333	0.058773577
Norm. supernatant t=0	0.033	-0.165	-0.0295	-0.053833333	0.101217999
Norm. supernatant t=16	0.081	0.102	0.009	0.064	0.048774994

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-<button type="button" class="btn btn-default btn-lg btn-block" style= "font-size: 35px;"><a href="https://2017.igem.org/Team:CMUQ/Results"> Results<a/></button>
+<button type="button" class="btn btn-default btn-lg btn-block" style= "font-size: 35px;"><a href="https://2017.igem.org/Team:CMUQ/Results"> Results</a></button>
+<p style="font-size: 20px;margin-left:40px; margin-right:40px;">The following results were obtained for the Dispersin B, which is an improvement on an Existing part: </p>
+<p style="font-size: 20px;margin-left:80px; margin-right:40px;">1. The results for the GFP fluorescence measured for three replicates of the LB (blank) as well as two constructs at excitation 585nm/emission 607nm </p>
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+<table style="width:50%; margin-left:100px;">
+  <tr>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th></th>
+    <th>Average</th>
+    <th>SD</th>
+    <th>t test</th>
+  </tr>
+  <tr>
+    <td>LB </td>
+    <td>-0.004</td>
+    <td>-0.0013</td>
+    <td>-0.055</td>
+    <td>-0.0201</td>
+    <td>0.030254421</td>
+    <td></td>
+  </tr>
+  <tr>
+    <td>t=0 supernatant</td>
+    <td>-0.034</td>
+    <td>0.009</td>
+    <td>0.005</td>
+    <td>0.006666667</td>
+    <td>0.023755701</td>
+    <td>0.328585241</td>
+  </tr>
+   <tr>
+    <td>t=16 supernatant</td>
+    <td>0.153</td>
+    <td>0.038</td>
+    <td>0.046</td>
+    <td>0.079</td>
+    <td>0.064210591</td>
+    <td>0.050130586</td>
+  </tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2017/b/b3/Dspb1.png" width=55% style="margin-left:100px;">
+<p style="font-size: 20px;margin-left:80px; margin-right:40px;">2. Averaging and normalizing the fluorescence readings measured from culture before pelleting the cells and then after pelleting the cells. </p>
+<table style="width:50%; margin-left:100px;">
+  <tr>
+    <th></th>
+    <th colspan="5">Fluorescence </th>
+  </tr>
+  <tr>
+    <td>pDP12 </td>
+    <td>-0.033</td>
+    <td>0.02</td>
+    <td>0.036</td>
+    <td>0.007666667</td>
+    <td>0.036115555</td>
+  </tr>
+  <tr>
+    <td>cells t=0</td>
+    <td>-0.029</td>
+    <td>0.008</td>
+    <td>-0.008</td>
+    <td>-0.009666667</td>
+    <td>0.018556221</td>
+  </tr>
+   <tr>
+    <td>cells t=16</td>
+    <td>0.09</td>
+    <td>0.032</td>
+    <td>0.07</td>
+    <td>0.064</td>
+    <td>0.02946184</td>
+  </tr>
+   <tr>
+    <td>supernatant t=0</td>
+    <td>0</td>
+    <td>-0.145</td>
+    <td>0.0065</td>
+    <td>-0.046166667</td>
+    <td>0.085653858</td>
+  </tr>
+    <tr>
+    <td>supernatant t=16</td>
+    <td>0.048</td>
+    <td>0.122</td>
+    <td>0.045</td>
+    <td>0.071666667</td>
+    <td>0.043615746</td>
+  </tr>
+  <th></th>
+  <th></th>
+  <th></th>
+  <th></th>
+  <th></th>
+  <th></th>
+  <tr>
+  </tr>
+    <tr>
+    <td>Norm. cells t=0</td>
+    <td>0.004</td>
+    <td>-0.012</td>
+    <td>-0.044</td>
+    <td>-0.017333333</td>
+    <td>0.024440404</td>
+  </tr>
+    <tr>
+    <td>Norm. cells t=16</td>
+    <td>0.123</td>
+    <td>0.012</td>
+    <td>0.034</td>
+    <td>0.056333333</td>
+    <td>0.058773577</td>
+  </tr>
+    <tr>
+    <td>Norm. supernatant t=0</td>
+    <td>0.033</td>
+    <td>-0.165</td>
+    <td>-0.0295</td>
+    <td>-0.053833333</td>
+    <td>0.101217999</td>
+  </tr>
+    <tr>
+    <td>Norm. supernatant t=16</td>
+    <td>0.081</td>
+    <td>0.102</td>
+    <td>0.009</td>
+    <td>0.064</td>
+    <td>0.048774994</td>
+  </tr>
+</table>
 </div>

Difference between revisions of "Team:CMUQ/Dspb Lab R"

Latest revision as of 02:03, 2 November 2017

Dspb Lab Results