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| − | <center> <h1> Emory</h1> </center>
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| − | <center><font size="5"><b>Project Description</b></font></center>
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| − | <p><font size="4" face="Georgia">The WaterHub at Emory University is a wastewater treatment plant on campus that utilizes bacteria and plants to recycle 400,000 gallons of water a day for non-potable use. Emory iGEM team is interested in using synthetic biology to help optimize their system to minimize maintenance processes. Currently, the WaterHub resorts to adding polyaluminum chloride to combat orthophosphate levels in some instances. The aim of our project is to engineer a bacteria that can combat their orthophosphate levels by creating a phosphate accumulating organism. By utilizing the Keio and ASKA <i>E. coli</i> collections, we plan to create an experimental strain of bacteria with high copy number plasmids of polyphosphate kinase and the exopolyphosphatase gene knocked out. We also plan to test the phosphate types and concentration levels within the WaterHub system. After engineering the bacteria, we hope to see the long-term efficiency of the cells’ phosphate uptake as well as survival within the WaterHub conditions. A secondary endeavor our team will pursue is the removal of excess calcium ions (Ca<sup>2+</sup>) from the water by exploring the potential of calmodulin-like proteins native to <i>E.coli</i>.</font></p>
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