Difference between revisions of "Team:CMUQ/InterLab"

 
(17 intermediate revisions by 2 users not shown)
Line 5: Line 5:
 
     <link href="https://fonts.googleapis.com/css?family=Montserrat" rel="stylesheet">
 
     <link href="https://fonts.googleapis.com/css?family=Montserrat" rel="stylesheet">
  
−
    <link href="//2015.igem.org/Team:Oxford/Assets/Bootstrap?action=raw&ctype=text/css" rel="stylesheet">
+
 
−
    <link href="//2015.igem.org/Team:Oxford/Assets/stylesheet?action=raw&ctype=text/css" rel="stylesheet">
+
−
    <link rel="stylesheet" href="//2015.igem.org/Team:Oxford/Assets/Font-Awesome?action=raw&ctype=text/css">
+
−
    <script type="text/javascript" src="//2015.igem.org/Team:Oxford/Assets/Bootstrapj?action=raw&ctype=text/javascript"></script>
+
−
    <script type="text/javascript" src="//2015.igem.org/Team:Oxford/Assets/contents?action=raw&ctype=text/javascript"></script>
+
−
 
+
−
    <script src="https://2015.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script>
+
−
 
+
 
<link rel='stylesheet prefetch' href='https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/css/bootstrap.min.css'>
 
<link rel='stylesheet prefetch' href='https://maxcdn.bootstrapcdn.com/bootstrap/3.3.5/css/bootstrap.min.css'>
  
Line 19: Line 12:
  
 
<style>
 
<style>
 +
@media (min-width: 768px) {
 +
    .dropdown:hover .dropdown-menu {
 +
    display: block;
 +
    }
 +
}
 +
.footer{
 +
    display: table;
 +
    text-align: center;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
}
 
body, html {
 
body, html {
 
     height: 100%;
 
     height: 100%;
 
}
 
}
 +
.centered {
 +
    position: absolute;
 +
    top: 200px;
 +
    left: 330px;
  
 +
  color: white;
 +
}
 
.main{
 
.main{
 
     /* The image used */
 
     /* The image used */
−
     background-image: url('#');
+
     background-image: url('https://static.igem.org/mediawiki/2017/9/97/IMG_9697.jpg');
  
 
     /* Full height */
 
     /* Full height */
Line 126: Line 136:
 
      .navbar-default.affix {
 
      .navbar-default.affix {
 
        padding: 0;
 
        padding: 0;
 +
margin-top: 9px;
 
        background-color: white;
 
        background-color: white;
 
        /* Add additional styles here for the COLLAPSED state */
 
        /* Add additional styles here for the COLLAPSED state */
Line 405: Line 416:
 
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#">PROJECT <span class="caret"> </span></a>
 
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#">PROJECT <span class="caret"> </span></a>
 
                 <ul class="dropdown-menu">
 
                 <ul class="dropdown-menu">
−
                            <li><a href="https://2017.igem.org/Team:CMUQ/descritpion">Description</a></li>
+
  <li><a href="https://2017.igem.org/Team:CMUQ/Description">Description</a></li>
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/design">Design</a></li>
+
                             <li><a href="https://2017.igem.org/Team:CMUQ/Design">Design</a></li>
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/safety">Safety</a></li>
+
                             <li><a href="https://2017.igem.org/Team:CMUQ/Safety">Safety</a></li>
 
                             <li><a href="https://2017.igem.org/Team:CMUQ/Demonstrate">Demonstrate</a></li>
 
                             <li><a href="https://2017.igem.org/Team:CMUQ/Demonstrate">Demonstrate</a></li>
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/Model">Model</a></li>
+
                             <li><a href="https://2017.igem.org/Team:CMUQ/Parts">Parts</a></li>
 
                             <li><a href="https://2017.igem.org/Team:CMUQ/Collaborations">Collaborations</a></li>
 
                             <li><a href="https://2017.igem.org/Team:CMUQ/Collaborations">Collaborations</a></li>
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/achievments">Achievements</a></li>
+
                             <li><a href="https://2017.igem.org/Team:CMUQ/JudgingForm">Judging Form </a></li>
 +
                           
 
                 </ul>
 
                 </ul>
 
             </li>
 
             </li>
 
             <li class="dropdown">
 
             <li class="dropdown">
−
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#"> PRACTICES <span class="caret"> </span></a>
+
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#"> COMMUNITY INVOLVEMENT <span class="caret"> </span></a>
 
                 <ul class="dropdown-menu">
 
                 <ul class="dropdown-menu">
 
                             <li><a href="https://2017.igem.org/Team:CMUQ/humanpractices">Human Practices</a></li>
 
                             <li><a href="https://2017.igem.org/Team:CMUQ/humanpractices">Human Practices</a></li>
 
                             <li><a href="https://2017.igem.org/Team:CMUQ/HP/Silver"> Silver HP </a></li>
 
                             <li><a href="https://2017.igem.org/Team:CMUQ/HP/Silver"> Silver HP </a></li>
 +
                            <li><a href="https://2017.igem.org/Team:CMUQ/HP/Gold_Integrated"> Gold HP </a></li>
 +
                            <li><a href="https://2017.igem.org/Team:CMUQ/Engagement"> Outreach</a></li>
 
                 </ul>
 
                 </ul>
 
             </li>
 
             </li>
 
             <li class="dropdown">
 
             <li class="dropdown">
−
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#"> WET LAB <span class="caret"> </span></a>
+
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#"> LAB WORK <span class="caret"> </span></a>
 
                 <ul class="dropdown-menu">
 
                 <ul class="dropdown-menu">
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/results">Overview & Results</a></li>
+
                              
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/notebook">Notebook</a></li>
+
                             <li><a href="https://2017.igem.org/Team:CMUQ/Notebook">Notebook</a></li>
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/protocols">Protocols</a></li>
+
                             <li><a href="https://2017.igem.org/Team:CMUQ/protocols">Procedures</a></li>
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/InterLab">InterLab</a></li>
+
                             <li><a href="https://2017.igem.org/Team:CMUQ/InterLab">InterLab Study</a></li>
−
                </ul>
+
                            <li><a href="https://2017.igem.org/Team:CMUQ/Results">Results</a></li>
−
            </li>
+
−
            <li class="dropdown">
+
−
                <a class="dropdown-toggle" data-toggle="dropdown" href="#">DRY LAB<span class="caret"> </span></a>
+
−
                <ul class="dropdown-menu">
+
−
                    <li><a href="https://2017.igem.org/Team:CMUQ/modelling">Modelling</a></li>
+
 
                 </ul>
 
                 </ul>
 
             </li>
 
             </li>
 +
           
 
             <li class="dropdown">
 
             <li class="dropdown">
 
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#"> TEAM <span class="caret"> </span></a>
 
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#"> TEAM <span class="caret"> </span></a>
 
                 <ul class="dropdown-menu">
 
                 <ul class="dropdown-menu">
−
                     <li><a href="https://2017.igem.org/Team:CMUQ/team">Team</a></li>
+
                     <li><a href="https://2017.igem.org/Team:CMUQ/Team">Team</a></li>
−
                             <li><a href="https://2017.igem.org/Team:CMUQ/attributions">Attributions</a></li>
+
                             <li><a href="https://2017.igem.org/Team:CMUQ/Attributions">Attributions</a></li>
 
                 </ul>
 
                 </ul>
 
             </li>
 
             </li>
Line 451: Line 461:
 
     <!-- /.container -->
 
     <!-- /.container -->
 
   </nav>
 
   </nav>
 +
  
 
<div class="main">
 
<div class="main">
 +
<div class="centered"> <h1>Interlab </h1> </div>
  
 
</div>
 
</div>
Line 458: Line 470:
 
<body>
 
<body>
  
 +
<div>
 +
<br>
 +
<br>
 +
<p style="font-family: 'Lora', serif; margin-right: 120px; margin-left:120px;font-size:25px;margin-top:30px;">
 +
iGEM CMUQ participated in the Inter Lab study for 2017 and submitted the results on time. The following are the two sheets, for plater reader experiment and the flow cytometer experiment, which were submitted:
 +
</p>
 +
<br>
 +
<div style="font-family: 'Montserrat', sans-serif;font-size:50px; text-align: center; class="button_01"><a href="https://static.igem.org/mediawiki/2017/8/8a/CMUQ_InterLab_2017_Measurements.pdf" target="_blank">Plater Reader </a></div>
 +
<br>
 +
<br>
 +
<br>
 +
<div style="font-family: 'Montserrat', sans-serif;font-size:50px; text-align: center; class="button_01"><a href="https://static.igem.org/mediawiki/2017/5/52/CMUQ_iGEM2017_Flow_Cytometry_Worksheet.pdf" target="_blank">Flow Cytometry</a></div>
 +
</div>
  
−
<div style = "padding: 35px;">
 
−
<p style= "font-size: 50px;"> BioSensor Lab </p>
 
  
−
<h3>A1: T7 salt sensor </h3>
 
−
<h4> Introduction </h4>
 
−
<p> The goal is amplify T7 salt sensor, digest it along with pSB1C3 plasmid with EcoRI and PstI</p>
 
−
<h4>Materials </h4>
 
−
<ul>
 
−
<li> Polymerase: Fusion Taq or Ampli tas (gold) </li>
 
−
<li>Primers: PrefixF  and SuffixR  </li>
 
−
<li>Nuclease free H2O </li>
 
−
<li>5xHF PCR Buffer </li>
 
−
<li>gBlock DNA </li>
 
−
<li> dNTPs</li>
 
−
<li>PCR tubes </li>
 
−
</ul>
 
−
 
−
<h4> Procedure </h4>
 
−
<p style= "font-size: 20px;> Preparation of reagents </p>
 
−
 
−
<ol>
 
−
<li> Resuspend gBLOCK in 20µL nuclease free dH2O</li>
 
−
<li> Resuspend primers to make 100µM stock then dilute to make a 10µM stock</li>
 
−
 
−
<li>Add the following reagents to a PCR tube: </li>
 
−
<li>Run the PCR machine </li>
 
−
 
−
<li> Digest amplified sequence with EcoRI and PstI </li>
 
−
<li> Digest pSB1C3 vector with EcoRI and PstI</li>
 
−
<li> If you clone into a vector that expresses a fluorescent protein, other than red since you are cloning in red, then colonies that still express that fluorescent protein will be negative clones and the red or white colonies will be the ones that you want.</li>
 
−
<li>Run on an agarose gel, cut out the bands and purify.  </li>
 
−
<li> Send clones for sequencing  </li>
 
−
</ol>
 
  
 +
<div class="footer">
 +
<hr> 
 +
<span class="glyphicon glyphicon-chevron-up"></span>
  
 +
</br>
 +
<img src = "https://static.igem.org/mediawiki/2017/e/e5/Occidental_Logo..png" width = "15%;" >
 +
<img src = "https://static.igem.org/mediawiki/2017/6/66/Logo_w-o_bg_green_mangroves.png" width = "20%;" >
 +
<img src = "https://static.igem.org/mediawiki/2017/f/fa/IDT_logo.cmuq.png" width = "20%;" >
 +
<img src = "https://static.igem.org/mediawiki/2017/5/53/Benchling-logo.png" width = "20%; " >
 
</div>
 
</div>
−
 
−
 
−
 
  
  

Latest revision as of 02:59, 2 November 2017

Interlab



iGEM CMUQ participated in the Inter Lab study for 2017 and submitted the results on time. The following are the two sheets, for plater reader experiment and the flow cytometer experiment, which were submitted: