Latest revision as of 03:06, 2 November 2017

Protocols

This page includes all our protocols that we made when we were cloning initially, as well as being a repository for protocols we made for experiments that did or didn't work to characterise our parts. Although a lot of them are simple protocols, and probably do not vary much from other teams', we have left them as we used them, so that other teams can get an indication of the sort of things we feel are important to include when you make protocols for your team. We used Benchling to write them, and it meant that demonstrators only had to explain things to one member of our team, rather than 12 different times!

Protocols for Initial Cloning

We wanted part of this page to summarise the steps that we took from designing our parts all the way through to sending them to iGEM to add to the registry. It is the sort of overview we would have found useful when we first started, and we hope it will help other teams.

Create DNA in Snapgene

Order DNA from IDT

PCR from G-Blocks

Run a Gel of the PCR

Digest and Ligate

Digest and Ligate Into Shipping Vector

Transform and Plate

Inoculate

Miniprep

Test Digest and Sequence

Send DNA to iGEM

Other Protocols

Below we have listed all the other protocols we used over the summer, they are linked to from throughout the wiki, and we hope other teams find them useful.

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+<p>This page includes all our protocols that we made when we were cloning initially, as well as being a repository for protocols we made for experiments that did or didn't work to characterise our parts. Although a lot of them are simple protocols, and probably do not vary much from other teams', we have left them as we used them, so that other teams can get an indication of the sort of things we feel are important to include when you make protocols for your team. We used Benchling to write them, and it meant that demonstrators only had to explain things to one member of our team, rather than 12 different times!</p>
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+<p> We wanted part of this page to summarise the steps that we took from designing our parts all the way through to sending them to iGEM to add to the registry. It is the sort of overview we would have found useful when we first started, and we hope it will help other teams.</p>
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+     <img class="img-responsive img-circle team-member-img sumaera_open" src="https://static.igem.org/mediawiki/2017/1/1d/T--Oxford--Protocols--Figure_11.png">
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      </div>
      </div>
-    <div class="col-sm-1">
      </div>
+<br>
+<br>
+<h2>Other Protocols</h2>
+<p>Below we have listed all the other protocols we used over the summer, they are linked to from throughout the wiki, and we hope other teams find them useful.</p>
+<br>
+<div class="row">
+<div class="col-sm-3">
+<a href="#demo" class="btn btn-info" data-toggle="collapse">Bits and Pieces</a>
+  <div id="demo" class="collapse">
+<ul>
+<li><a href="https://static.igem.org/mediawiki/2017/b/b6/T--Oxford--Protocols--bap1.pdf">gBlock Resuspending</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/b/b7/T--Oxford--Protocols--bap2.pdf">Making Loading Dye</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/9/90/T--Oxford--Protocols--bap3.pdf">Making 1kb+ Ladder</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/8/8a/T--Oxford--Protocols--bap4.pdf">Making Plates</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/f/f1/T--Oxford--Protocols--bap5.pdf">Making Competent Cells</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/2/27/T--Oxford--Protocols--bap6.pdf">Making Antibiotics</a> </li>
+</ul>
+</div>
+</div>
+<div class="col-sm-3">
+<a href="#demoa" class="btn btn-info" data-toggle="collapse">Various Types of PCR</a>
+  <div id="demoa" class="collapse">
+<ul>
+<li><a href="https://static.igem.org/mediawiki/2017/5/5b/T--Oxford--Protocols--pcr1.pdf">Normal PCR</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/f/ff/T--Oxford--Protocols--pcr2.pdf">Colony PCR</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/4/4f/T--Oxford--Protocols--pcr3.pdf">Quick-change PCR</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/9/96/T--Oxford--Protocols--pcr4.pdf">Phusion PCR</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/a/a3/T--Oxford--Protocols--pcr5.pdf">Overlap Extension PCR</a></li>
+</ul>
+</div>
+</div>
+<div class="col-sm-3">
+<a href="#demob" class="btn btn-info" data-toggle="collapse">Protein Purification</a>
+  <div id="demob" class="collapse">
+<ul>
+<li><a href="https://static.igem.org/mediawiki/2017/2/2e/T--Oxford--Protocols--purification1.pdf">Making Buffers for Purification</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/4/4a/T--Oxford--Protocols--purification2.pdf">Using the Avanti Centrifuge</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/a/aa/T--Oxford--Protocols--purification3.pdf">Using the Berks Cell Disruptor</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/c/cf/T--Oxford--Protocols--purification4.pdf">E.g. of a Purification TEV-mCherry-His</a> </li>
+</ul>
+</div>
+</div>
+<div class="col-sm-2">
+<a href="#democ" class="btn btn-info" data-toggle="collapse">Microscopy</a>
+  <div id="democ" class="collapse">
+<!--<p>In brief, mid-log phase cells were immobilised on a thin layer of 1% w/v agarose in water on a slide with a cover slip. Microscopy was carried out using a Nikon TE200 inverted microscope with filter sets for CFP, GFP, YFP and mCherry from Chroma. Images were acquired on a Hamamatsu Orca ER CCD camera and processed using SimplePCI software. All biological samples to be compared were grown simultaneously and the microscope settings, exposure times and post processing of each image were identical.</p>-->
+<ul>
+<li><a href="https://static.igem.org/mediawiki/2017/9/90/T--Oxford--Protocols--microscopy1.pdf">Making Slides</a> </li>
+<li><a href="https://static.igem.org/mediawiki/2017/d/d4/T--Oxford--Protocols--microscopy2.pdf">Using the Fluorescence Microscope</a> </li>
+</ul>
+</div>
+</div>
+<div class="col-sm-1">
+<a href="#demod" class="btn btn-info" data-toggle="collapse">OMVs</a>
+  <div id="demod" class="collapse">
+<ul>
+<li><a href="https://static.igem.org/mediawiki/2017/1/1a/T--Oxford--Protocols--OMV.pdf">OMV Extraction</a> </li>
+</ul>
+</div>
+</div>
+</div>
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@@ Line 395: / Line 347: @@
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+{{Oxford/footer}}

Difference between revisions of "Team:Oxford/Protocols"