As detailed on our "Experiments" page, we measured chrR6 chromium reduction activity using DPC assay. The strong r² value of 0.9944 for the linear trend line of our standard DPC curve indicates that this is an accurate Cr(VI) detection method within this range of concentrations. Therefore, when we investigate the reduction efficiencies of our reductases, we will use initial Cr(VI) concentrations within this range.

Figure 4: DPC standard curve

Figure 1: Chromium reduction by E. coli MG1655 transformed with chrR6 and sulfate transport complex.

cysPUWA Effect on Cell Membrane Permeability to Cr(VI)

Kill Switch Failsafe Mechanism

Figure 1: IPTG induces toxin expression leading to cell death

Figure 4: IPTG-induced mCherry fluorescence vs. time

Figure 4: IPTG-induced mCherry fluorescence/OD vs. time

PgntK Promoter Activity

Project Achievements