Difference between revisions of "Team:Amazonas Brazil/Project"

 
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<title>Wiki_iGEM_Amazonas</title>
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  <title>Wiki_iGEM_Amazonas</title>
  
 
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         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
 
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         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Model">Modelling</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Model">Modelling</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/InterLab">Interlab</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/InterLab">Interlab</a></li>
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         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">Design</a></li>
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         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">Applied Design</a></li>
 
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          <div class="subcontainer" id ="top">
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    <a class="Menu" id="Link_Menu1" href="https://2017.igem.org/Team:Amazonas_Brazil/Project">PROJECT</a>
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              <a class="Menu" id="Link_Menu1" href="https://2017.igem.org/Team:Amazonas_Brazil/Project">PROJECT</a>
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    <a class="Menu" id="Link_Menu2" href="https://2017.igem.org/Team:Amazonas_Brazil/Biobricks">BIOBRICKS</a>
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              <a class="Menu" id="Link_Menu2" href="https://2017.igem.org/Team:Amazonas_Brazil/Biobricks">BIOBRICKS</a>
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    <a class="Menu" id="Link_Menu3" href="https://2017.igem.org/Team:Amazonas_Brazil/Software">SOFTWARE</a>
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              <a class="Menu" id="Link_Menu3" href="https://2017.igem.org/Team:Amazonas_Brazil/Software">SOFTWARE</a>
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    <a class="Menu" id="Link_Menu4" href="https://2017.igem.org/Team:Amazonas_Brazil/Human_Practices">HUMAN PRACTICES</a>
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              <a class="Menu" id="Link_Menu4" href="https://2017.igem.org/Team:Amazonas_Brazil/Human_Practices">HUMAN PRACTICES</a>
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<a class="Menu" id="Link_Menu5" href="https://2017.igem.org/Team:Amazonas_Brazil/Achievements">ACHIEVEMENTS</a>
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              <a class="Menu" id="Link_Menu5" href="https://2017.igem.org/Team:Amazonas_Brazil/Achievements">ACHIEVEMENTS</a>
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<a class="Menu" id="Link_Menu6" href="https://2017.igem.org/Team:Amazonas_Brazil/People">PEOPLE</a>
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              <a class="Menu" id="Link_Menu6" href="https://2017.igem.org/Team:Amazonas_Brazil/People">PEOPLE</a>
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   </header>
 
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<div class="hero-full-container background-image-container white-text-container" style="background-image: url(https://static.igem.org/mediawiki/2017/1/11/T--Amazonas_Brazil--PROJECT.jpg); " >
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  <div class="hero-full-container background-image-container white-text-container" style="background-image: url(https://static.igem.org/mediawiki/2017/1/11/T--Amazonas_Brazil--PROJECT.jpg); ">
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  <div class="container">
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          <div class="text-content  text-center">
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            <div class="text-content  text-center">
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            <h2 class="Page_Title" align="middle">PROJECT</h2>
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              <h2 class="Page_Title" align="middle">PROJECT</h2>
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          <h2 class="Page_Subtitle" align="middle">pCRISPeasy</h2>
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              <h2 class="Page_Subtitle" align="middle">pCRISPeasy</h2>
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            </div>
 
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     </div>
 
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             <h1 class="First"style="text-align: center;height: 2.3em;">Navigate into CRISPeasy roadmap</h1>
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             <h1 class="First" style="text-align: center;height: 2.3em;">Navigate into CRISPeasy roadmap</h1>
 
             <div>
 
             <div>
  
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               <div style='position:relative;top:0px; left:0px;'> <!-- div para toda a imagem central -->
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              <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
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                <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto" src="https://static.igem.org/mediawiki/2017/7/7b/Amazonas_Brazil_Project_Roadmap.png" />
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                          src="https://static.igem.org/mediawiki/2017/a/ab/Fluxo.png"/>
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                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Human_Practices">
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                  <!-- div para imagens que se sobrepoem -->
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                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/HP/Gold_Integrated">
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto; width:150px; height:50px;"
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto; width:150px; height:50px;"
 
                                               src="https://static.igem.org/mediawiki/2017/1/1a/1_HumanPratices.png"/>
 
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                   </a>
 
                   </a>
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                  <!-- div para imagens que se sobrepoem -->
 
                   <a href="#Specifications">
 
                   <a href="#Specifications">
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                                               src="https://static.igem.org/mediawiki/2017/3/37/2_Specifications.png"/>
 
                                               src="https://static.igem.org/mediawiki/2017/3/37/2_Specifications.png"/>
 
                   </a>
 
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                <div style='position:absolute;top:18%; left:66%;'>
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                  <!-- div para imagens que se sobrepoem -->
 
                   <a href="#Design">
 
                   <a href="#Design">
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                                               src="https://static.igem.org/mediawiki/2017/c/c0/3_Design.png"/>
 
                                               src="https://static.igem.org/mediawiki/2017/c/c0/3_Design.png"/>
 
                   </a>
 
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                <div style='position:absolute;top:75%; left:66%;'>
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                  <!-- div para imagens que se sobrepoem -->
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Model">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Model">
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                                               src="https://static.igem.org/mediawiki/2017/7/79/4_Modelling.png"/>
 
                                               src="https://static.igem.org/mediawiki/2017/7/79/4_Modelling.png"/>
 
                   </a>
 
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                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Assembly">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Assembly">
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                                               src="https://static.igem.org/mediawiki/2017/4/4c/5_Assembly.png"/>
 
                                               src="https://static.igem.org/mediawiki/2017/4/4c/5_Assembly.png"/>
 
                   </a>
 
                   </a>
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                <div style='position:absolute;top:76%; left:24%;'>
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                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Testing">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Testing">
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                                               src="https://static.igem.org/mediawiki/2017/a/a4/6_Testing.png"/>
 
                                               src="https://static.igem.org/mediawiki/2017/a/a4/6_Testing.png"/>
 
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                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">
 
                   <a href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                       <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
 
                                               src="https://static.igem.org/mediawiki/2017/3/38/7_Implementation.png"/>
 
                                               src="https://static.igem.org/mediawiki/2017/3/38/7_Implementation.png"/>
 
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               <h1 style=" margin: 0;padding-top: 0.1em;"id="Specifications"></h1>
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               <h1 style=" margin: 0;padding-top: 0.1em;" id="Specifications"></h1>
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               <h2 style="margin-top: 1.4em;">Specifications</h2>
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               <h3>Our proposal is to provide a bacterial genome editing approach based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR</h3>
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               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
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               <p class="p">
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              src="https://static.igem.org/mediawiki/2017/f/fa/Amazonas_Brazil_Project_Specifications.png">
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                The scientific community had achieved great results using CRISPR-Cas9 mediated genome editing techniques. While these methods are moving forward, some basic methodological aspects related to this approach could be further improved to expand this scientific revolution.
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In prokaryotes, the genome editing based on CRISPR/Cas9 machinery require quite laborious days of work, the system is weakly founded on bioengineering principles, demands lots of steps, each one carrying a part of the CRISPR/Cas9 apparatus. In addition, off-target events happen frequently, the machinery post-effect in the cell isn’t entirely known yet, among others. To legitimate this information, we integrated our project design with other iGEM's teams who had worked with CRISPR/Cas9 in previous years. You can read more about what we found here (LINK PRA INTEGRADA)
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The decoupling of all these shortcomings paved the way to the project's delineation and we elected to focus on a challenge: the lack of standardization, which is a real-world problem - included but not limited to SynBio. Then, to optimize the previous methods, we designed a trustworthy, well functioning standardized framework based on BioBrick parts called: the CRISPeasy toolbox.
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               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
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              src="https://static.igem.org/mediawiki/2017/1/14/Amazonas_Brazil_Project_Prokaryotes.png">
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               <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
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              src="https://static.igem.org/mediawiki/2017/3/3f/Amazonas_Brazil_Project_Events.png">
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              <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
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              src="https://static.igem.org/mediawiki/2017/5/5b/Amazonas_Brazil_Project_Lack.png">
  
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                </p>
 
 
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            <h1 style=" margin: 0;padding-top: 0.1em;" id="Design" ></h1>
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              <h1 style=" margin: 0;padding-top: 0.1em;" id="Design"></h1>
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            <h2 style="margin-top: 1.4em;">Design</h2>
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            <h3>Check all the parts involved and our approaches with closer details!</h3>
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              <img alt="Erro" style="width: 80%;height: 80%;margin-left: 10%"
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              <p class="p">
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              src="https://static.igem.org/mediawiki/2017/b/b6/Amazonas_Brazil_Substitui%C3%A7%C3%A3o_Design.png">
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                Cas9 (BBa_K2457001): Standardized Cas9 device, regulated by the AraC_pBAD regulatory module.
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                In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex
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            <!--  <p class="p">Adicionar aqui</p> -->
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                which cleaves specifics target sequences from DNA, leading to double-strand breaks. Through cell repair
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                pathways, it will lays the road to genomic editing in living cells.
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