Difference between revisions of "Team:Northwestern/Lab"

 
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<body>  
 
<body>  
  
<br> <br>  
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<br>  
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<center><h1 style="padding-top:2%; padding-right: 15%; padding-left:15%"><br><br>Lab Notebook</h3></center>
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<br>
  
 
<div id="nestedAccordion" class="w3-center">
 
<div id="nestedAccordion" class="w3-center">
   <button class="w3-button w3-block"> Weekly Summaries</button>
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   <button class="w3-button w3-block panel-collapse collapse" style="font-size:30px; padding-top: 2%; padding-bottom: 2%"> Weekly summaries</button>
     <div>
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     <div style="display:none;">
         <h3> Week 1/2 </h3>
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         <h3 class="w3-button w3-block"> Week 1 and 2</h3>
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/archive/3/3d/20170802184027%21T--Northwestern--Week2.pdf" type="application/pdf" width="75%" height="600px">
 
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</object> </center> </div>
         <h3>Week 3</h3>
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         <h3 class="w3-button w3-block"> Week 3</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/d/d9/T--Northwestern--Week3.pdf" type="application/pdf" width="75%" height="600px">
 
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         <h3>Week 4</h3>
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         <h3 class="w3-button w3-block"> Week 4</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/6/66/T--Northwestern--Week4.pdf" type="application/pdf" width="75%" height="600px">
 
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</object> </center> </div>
           <h3>Week 5</h3>
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           <h3 class="w3-button w3-block"> Week 5</h3 >
 
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</object> </center> </div>
           <h3>Week 6</h3>
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           <h3 class="w3-button w3-block"> Week 6</h3 >
 
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           <h3>Week 7</h3>
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           <h3 class="w3-button w3-block"> Week 7</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/b/b9/T--Northwestern--Week7.pdf" type="application/pdf" width="75%" height="600px">
 
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        <h3 class="w3-button w3-block"> Week 8-9</h3 >
 +
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        <h3 class="w3-button w3-block"> Week 10</h3 >
 +
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     </div>
  <button class="w3-button w3-block"> Interlab </button>
 
    <div>
 
        <h3><center><object data="https://static.igem.org/mediawiki/2017/1/1c/T--Northwestern--Interlab.pdf" type="application/pdf" width="50%" height="600px">
 
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    </div>
 
<button class="w3-button w3-block"> OMV Fusion Project  </button>
 
    <div> </div>
 
  
  <button class="w3-button w3-block"> Protocols  </button>
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  <button class="w3-button w3-block" style="font-size : 30px; padding-top: 2%; padding-bottom: 2%"> Protocols  </button>
     <div>  
+
     <div style="display:none;">  
     <h3> Making TAE Buffer </h3>  
+
     <h3 id = "input" class="w3-button w3-block"> Making TAE buffer</h3 >  
 
<div> <center><object data="https://static.igem.org/mediawiki/2017/c/ca/T--Northwestern--10xTAE.pdf
 
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</object> </center> </div>  
 
</object> </center> </div>  
  
     <h3> Culture Innoculation </h3>  
+
     <h3 class="w3-button w3-block"> Culture Innoculation</h3 >
 
       <div>  <center><object data="https://static.igem.org/mediawiki/2017/7/72/T--Northwestern--CultureInoculation.pdf
 
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"></a>
 
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</object> </center> </div>  
 
</object> </center> </div>  
  
     <h3> Gemstone Dye Protocol </h3>  
+
     <h3 class="w3-button w3-block"> Gemstone dye protocol</h3 >  
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/f/f7/T--Northwestern--GemstoneDye.pdf
 
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       <h3> Fractionation </h3>  
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       <h3 class="w3-button w3-block"> Fractionation</h3 >
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/1/18/T--Northwestern--Fractionation.pdf
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/1/18/T--Northwestern--Fractionation.pdf
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"></a>
 
"></a>
 
</object> </center>  </div>  
 
</object> </center>  </div>  
 
      
 
      
       <h3> LB + Plate </h3>  
+
       <h3 class="w3-button w3-block"> LB + Plate</h3 >
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/d/da/T--Northwestern--LBPlate.pdf
 
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</object> </center> </div>  
 
</object> </center> </div>  
  
       <h3> Supplememnted Media </h3>  
+
       <h3 class="w3-button w3-block"> Supplemented Media</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/7/73/T--Northwestern--M9SupplementedMedia.pdf
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/7/73/T--Northwestern--M9SupplementedMedia.pdf
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</object> </center> </div>  
 
          
 
          
         <h3> Transformations </h3>  
+
         <h3 class="w3-button w3-block"> Transformations</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/f/f8/T--Northwestern--TransformationProtocol.pdf
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/f/f8/T--Northwestern--TransformationProtocol.pdf
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</object> </center> </div>
 
</object> </center> </div>
 
          
 
          
           <h3> Miniprep (Promega) </h3>  
+
           <h3 class="w3-button w3-block"> Miniprep (Promega)</h3 >  
 
           <div> <center><object data="https://static.igem.org/mediawiki/2017/9/9b/T--Northwestern--MiniprepPromega.pdf
 
           <div> <center><object data="https://static.igem.org/mediawiki/2017/9/9b/T--Northwestern--MiniprepPromega.pdf
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</object> </center> </div>  
 
</object> </center> </div>  
 
          
 
          
           <h3> OMV Purification </h3>
+
           <h3 class="w3-button w3-block"> OMV Purification</h3 >
 
           <div> <center><object data="https://static.igem.org/mediawiki/2017/e/e0/T--Northwestern--OMVPurification.pdf
 
           <div> <center><object data="https://static.igem.org/mediawiki/2017/e/e0/T--Northwestern--OMVPurification.pdf
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</object> </center> </div>  
 
</object> </center> </div>  
 
+
<h3 class="w3-button w3-block">PCR for Removal of His6 Tag from DsbA</h3 >
            <h3> PCR for Removal of His6 Tag from DsbA </h3>  
+
 
             <div> <center><object data="https://static.igem.org/mediawiki/2017/9/91/T--Northwestern--PCRHisRemoval.pdf
 
             <div> <center><object data="https://static.igem.org/mediawiki/2017/9/91/T--Northwestern--PCRHisRemoval.pdf
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</object> </center> </div>  
 
</object> </center> </div>  
  
             <h3> Sequencing </h3>  
+
             <h3 class="w3-button w3-block">Sequencing</h3 >
 
             <div> <center><object data="https://static.igem.org/mediawiki/2017/7/71/T--Northwestern--Sequencing.pdf
 
             <div> <center><object data="https://static.igem.org/mediawiki/2017/7/71/T--Northwestern--Sequencing.pdf
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</object> </center> </div>  
 
</object> </center> </div>  
  
             <h3> 2xYT </h3>  
+
             <h3 class="w3-button w3-block">2xYT</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/5/54/T--Northwestern--RecipeFor2xYT.pdf
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/5/54/T--Northwestern--RecipeFor2xYT.pdf
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</object> </center> </div>  
 
</object> </center> </div>  
 
            
 
            
             <h3> Self-circularization of Linear DNA </h3>  
+
             <h3 class="w3-button w3-block">Self-circularization of linear DNA</h3 >  
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/b/b3/T--Northwestern--SelfCircularization.pdf
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/b/b3/T--Northwestern--SelfCircularization.pdf
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"></a>
 
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</object> </center> </div>
 
</object> </center> </div>
  
             <h3> Spectinmyocin </h3>  
+
             <h3 class="w3-button w3-block">Spectinmyocin</h3 >
 
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              <h3> Tyler's Transformation and FLuorescence </h3>  
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"></a>
 
"></a>
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Latest revision as of 03:55, 2 November 2017

Northwestern Template Northwestern Template

Northwestern Template



Lab Notebook


Week 1 and 2

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Week 3

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Week 4

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Week 5

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Week 6

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Week 7

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Week 8-9

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Week 10

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Making TAE buffer

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Culture Innoculation

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Gemstone dye protocol

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Fractionation

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LB + Plate

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Supplemented Media

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Transformations

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Miniprep (Promega)

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OMV Purification

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PCR for Removal of His6 Tag from DsbA

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Sequencing

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2xYT

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Self-circularization of linear DNA

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Spectinmyocin

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Tyler's Transformation and Fluorescence

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