Difference between revisions of "Team:CMUQ/HP/Gold Integrated"

 
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                 <a class="dropdown-toggle" data-toggle="dropdown" href="#">PROJECT <span class="caret"> </span></a>
 
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                            <li><a href="https://2017.igem.org/Team:CMUQ/Description">Description</a></li>
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                             <li><a href="https://2017.igem.org/Team:CMUQ/Design">Design</a></li>
 
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1. Job title, experience and nature of work
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CMUQ team interviewed Taher Alkilani, a Chemical and Biocorrosion engineer at Occidental Petroleum, Qatar to learn more about the real problems that face oil companies as a result of SRB biofilms
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Taher Alkilani, oil and gas professional with 21 years experience in process, production, corrosion & scale. Taher is an expert in corrosion control; in depth hands-on experience in Microbial Induced Corrosion (MIC) monitoring and control, pipelines integrity management, well completion integrity management, failure analysis and materials selection.
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Bacteria can <b>sense</b> a vast range of environmental signals, from the concentrations of nutrients and toxins to oxygen levels, pH and <b>osmolarity</b>. For our project, we plan to genetically engineer both <b>sensor and remediation bacteria</b> to sense osmolarity of the water used to cool oil pipelines and then to secrete an enzyme to degrade the extracellular matrix of the biofilm
 
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Halanaerobium which is the major SRB found in these pipelines live on high salt concentrations (optimally at NaCl concentrations 1.7–2.5 M and requiring a minimum of 0.3–1.7 M NaCl for growth). Therefore, we plan to use water samples obtained from an oil extraction company in Qatar to determine the exact concentrations required for optimal growth of the SRB’s. We plan to use this data for determining if fresh water can be used to kill these bacteria. If successful, this idea can work as a novel method for non-invasive treatment of biofilms in oil-cooling pipelines. We will construct a salt biosensor using the osmo-responsive promoter of the ProU operon to drive a Red Fluorescent Protein (RFP) expression, which can be measured using fluorescence and a plate reader. This will allow us to monitor the osmolarity of outflow to determine if the SRB are likely to grow. After osmolarity biosensor is added, samples from the water can be taken frequently and the RFP’s fluorescence can be measured using simple fluorescence measurement in a plate reader.
 
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<div style="font-family: 'Montserrat', sans-serif;font-size:50px; text-align: center; class="button_01"><a href="https://static.igem.org/mediawiki/2017/4/42/Oxy_interview_pdf.pdf" target="_blank">Read the Interview here</a></div>
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If salt is still high after mixing with fresh water we can then use the ProU induction to increase expression of the Dispersin B (DspB) gene, which codes for a protein that digests the polysaccharides, a major component of the gram-positive bacterial cell wall and biofilms.
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Latest revision as of 03:58, 2 November 2017

Gold Integrated

CMUQ team interviewed Taher Alkilani, a Chemical and Biocorrosion engineer at Occidental Petroleum, Qatar to learn more about the real problems that face oil companies as a result of SRB biofilms