Difference between revisions of "Team:CSU Fort Collins"

 
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<p>Research performed in the last 50 years or so has forced humanity to reconsider its gluttonous appetite for petrol based products and energy. The effects of this "appetite" have been profound, scientifically undeniable, and perhaps the biggest threat humanity has faced.</p>
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<p>The answer: synthetic biology. Like any emerging field, synthetic biology is experiencing an explosion of novel methods and ideas. We have focused our efforts on altering the metabolic process of the archaea <i>Thermococcus kodakarensis</i> to produce the well-known terpenoid, limonene. Limonene is of great interest due to its myriad of industrial uses, including biofuels and plastics. Usage of an archaeal organism's mevalonate pathway allows for a simple incursion of one extra enzyme (limonene synthase) to convert GPP into limonene. Gas chromatography and western blot methods were used to quantify limonene concentration and detect gene expression respectively, in the altered <i>T. kodakarensis.</i> Limonene shows great potential for the future of energy and plastics, and archaea could be the key.</p>
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<p>Research done in the last 50 years or so has forced humanity to reconsider its gluttonous appetite for petrol based products and energy. The effects of this "appetite" have been profound, scientifically undeniable, and perhaps the biggest threat humanity has faced. The answer; synthetic biology. Like any emerging field, synthetic biology is experiencing an explosion of novel methods and ideas. We have focused our efforts on altering the metabolic process of the archaea Thermococcus kodakarensis to produce pragmatic amounts of the well-known terpenoid, limonene. Limonene is of great interest due to its myriad of industrial uses, namely as a biofuel. Usage of an archaeal organism's mevalonate pathway allows for a simple incursion of one extra enzyme (limonene synthase) to convert GPP into limonene. Gas chromatography and western blot methods were used to quantify limonene concentration and detect gene expression respectively, in the altered T. kodakarensis. </p>
 
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
 
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
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<h5> Uploading pictures and files </h5>
 
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
 
  
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<a href="https://2017.igem.org/Special:Upload">
 
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UPLOAD FILES
 
 
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Latest revision as of 03:59, 2 November 2017

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Research performed in the last 50 years or so has forced humanity to reconsider its gluttonous appetite for petrol based products and energy. The effects of this "appetite" have been profound, scientifically undeniable, and perhaps the biggest threat humanity has faced.

The answer: synthetic biology. Like any emerging field, synthetic biology is experiencing an explosion of novel methods and ideas. We have focused our efforts on altering the metabolic process of the archaea Thermococcus kodakarensis to produce the well-known terpenoid, limonene. Limonene is of great interest due to its myriad of industrial uses, including biofuels and plastics. Usage of an archaeal organism's mevalonate pathway allows for a simple incursion of one extra enzyme (limonene synthase) to convert GPP into limonene. Gas chromatography and western blot methods were used to quantify limonene concentration and detect gene expression respectively, in the altered T. kodakarensis. Limonene shows great potential for the future of energy and plastics, and archaea could be the key.