Difference between revisions of "Team:SZU-China/Parts"

 
(6 intermediate revisions by 2 users not shown)
Line 176: Line 176:
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232000">BBa_K2232000</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232000">BBa_K2232000</a></td>
−
                             <td>TSLV1-CA-&#945;</td>
+
                             <td>TSLV1-CA</td>
 
                             <td>Basic</td>
 
                             <td>Basic</td>
  
Line 184: Line 184:
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232005">BBa_K2232005</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232005">BBa_K2232005</a></td>
−
                             <td>OF4-CA-&#945;</td>
+
                             <td>OF4-CA</td>
 
                             <td>Basic</td>
 
                             <td>Basic</td>
  
Line 201: Line 201:
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232002">BBa_K2232002</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232002">BBa_K2232002</a></td>
−
                             <td>c125-nhaC</td>
+
                             <td>C125-nhaC</td>
 
                             <td>Basic</td>
 
                             <td>Basic</td>
  
−
                             <td>Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus c125</i></td>
+
                             <td>Coding sequence for Na<sup>+</sup> - H<sup>+</sup> antiporter nhaC from<i> Bacillus Subtilus C125</i></td>
  
 
                         </tr>
 
                         </tr>
Line 218: Line 218:
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232003">BBa_K2232003</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232003">BBa_K2232003</a></td>
−
                             <td>c125-TupA</td>
+
                             <td>C125-tupA</td>
 
                             <td>Basic</td>
 
                             <td>Basic</td>
  
−
                             <td style="width:300px;padding-left:5px">Coding sequence for another Na<sup>+</sup> - H<sup>+</sup> antiporter TupA from<i> Bacillus Subtilus c125 </i></td>
+
                             <td style="width:300px;padding-left:5px">Coding sequence for another Na<sup>+</sup> - H<sup>+</sup> antiporter TupA from<i> Bacillus Subtilus C125 </i></td>
  
 
                         </tr>
 
                         </tr>
Line 261: Line 261:
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232011">BBa_K2232011</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232011">BBa_K2232011</a></td>
−
                             <td>RBS-c125-TupA-T1</td>
+
                             <td>RBS-C125-tupA-T1</td>
 
                             <td>Composite</td>
 
                             <td>Composite</td>
  
−
                             <td style="width:300px;padding-left:5px">Native sequence from <i>  Mycobacterium NBB4 </i>, contained within the ethylene metabolism operon</td>
+
                             <td style="width:300px;padding-left:5px">Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125</td>
  
 
                         </tr>
 
                         </tr>
Line 272: Line 272:
 
                             <td>Composite</td>
 
                             <td>Composite</td>
  
−
                             <td style="width:300px;padding-left:5px">Coding sequence of EtnR1 from <i>Mycobacterium NBB4 </i>, codon-optimised for expression in <i> E. coli</i> K12</td>
+
                             <td style="width:300px;padding-left:5px">The gene nhaC from the Bacillus pseudofirmus OF4 encodes the Na+/H+ antiporter</td>
  
 
                         </tr>
 
                         </tr>
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232013">BBa_K2232013</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232013">BBa_K2232013</a></td>
−
                             <td>p43-nhaC-T1</td>
+
                             <td>P43-nhaC-T1</td>
 
                             <td>Composite</td>
 
                             <td>Composite</td>
  
−
                             <td style="width:300px;padding-left:5px">Coding sequence of EtnR1 from <i>Mycobacterium </i> NBB4, codon-optimised for expression in<i> E. coli </i>K12</td>
+
                             <td style="width:300px;padding-left:5px">This part is the Na+/H+ antiporter coding sequence (CDS) from the B. subtilis strain 168</td>
  
 
                         </tr>
 
                         </tr>
Line 286: Line 286:
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232014">BBa_K2232014</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232014">BBa_K2232014</a></td>
−
                             <td>p43-TSLV1-CA-&#945;</td>
+
                             <td>P43-TSLV1-CA</td>
 
                             <td>Composite</td>
 
                             <td>Composite</td>
  
Line 294: Line 294:
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232015">BBa_K2232015</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232015">BBa_K2232015</a></td>
−
                             <td>P43-OF4-CA-&#945;</td>
+
                             <td>P43-OF4-CA</td>
 
                             <td>Composite</td>
 
                             <td>Composite</td>
  
Line 303: Line 303:
 
                         <tr>
 
                         <tr>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232026">BBa_K2232026</a></td>
 
                             <td><a href="http://parts.igem.org/Part:BBa_K2232026">BBa_K2232026</a></td>
−
                             <td>p43-CA-β</td>
+
                             <td>P43-CA-β</td>
 
                             <td>Composite</td>
 
                             <td>Composite</td>
  
Line 352: Line 352:
 
                         <tr>
 
                         <tr>
 
                             <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232022">BBa_K2232022</a><br /><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1465205" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K2465205</a></td>
 
                             <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232022">BBa_K2232022</a><br /><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1465205" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K2465205</a></td>
−
                             <td>p43-csos3</td>
+
                             <td>RBS-CsoS3-T1</td>
 
                             <td>Composite</td>
 
                             <td>Composite</td>
  
Line 360: Line 360:
 
                         <tr>
 
                         <tr>
 
                             <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232025">BBa_K2232025</a><br /><a href="http://parts.igem.org/Part:BBa_K302035" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K302035</a></td>
 
                             <td style="padding-top: 5px; padding-bottom: 5px;"><a href="http://parts.igem.org/Part:BBa_K2232025">BBa_K2232025</a><br /><a href="http://parts.igem.org/Part:BBa_K302035" style="color:#808080;font-size:12px!important;font-style:italic">BBa_K302035</a></td>
−
                             <td>mazEF</td>
+
                             <td>Sucrose-limitation induced kill switch</td>
 
                             <td>Composite</td>
 
                             <td>Composite</td>
  
Line 367: Line 367:
 
                         </tr>
 
                         </tr>
 
                     </tbody>
 
                     </tbody>
−
 
+
Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125
 
                 </table>
 
                 </table>
  

Latest revision as of 04:01, 2 November 2017

PARTS



Basic Parts

This year, our team ultilizes 2 kinds of new parts: one is for carbonic anhydrase expression, another is for Na+-H+ antiporter, which can help improve alkaline resistance.You can click on the part number to see the details.


Part Number Name Type Part Description
BBa_K2232000 TSLV1-CA Basic Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus TSLV1
BBa_K2232005 OF4-CA Basic Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus OF4
BBa_K2232001 OF4-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus OF4
BBa_K2232002 C125-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus C125
BBa_K2232007 BS168-nhaC Basic Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus 168
BBa_K2232003 C125-tupA Basic Coding sequence for another Na+ - H+ antiporter TupA from Bacillus Subtilus C125





Composite Parts

The 2 kinds of basic parts described above is assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.


Part Number Name Type Part Description
BBa_K2232011 RBS-C125-tupA-T1 Composite Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125
BBa_K2232012 P43-OF4-nhaC-T1 Composite The gene nhaC from the Bacillus pseudofirmus OF4 encodes the Na+/H+ antiporter
BBa_K2232013 P43-nhaC-T1 Composite This part is the Na+/H+ antiporter coding sequence (CDS) from the B. subtilis strain 168
BBa_K2232014 P43-TSLV1-CA Composite Coding region of the pink mutant version of amilCP
BBa_K2232015 P43-OF4-CA Composite Coding region of the purple mutant version of amilCP
BBa_K2232026 P43-CA-β Composite Coding region of the green mutant version of amilCP





Improved Parts

This year, our team have improved the existing parts by either chracterization or optimize their sequence. The improved parts (in black) and original existing parts (in grey) are presented below. You can click on the part number to see the details.


Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125
Part Number Name Type Part Description Improvement
BBa_K2232020
BBa_K911008		 PsspB-gerAa Composite Germination-promoting receptor (gerA) expression system Optimize its sequence
BBa_K2232022
BBa_K2465205		 RBS-CsoS3-T1 Composite Carbonic anhydrase (csoS3) expression system Optimize its sequence
BBa_K2232025
BBa_K302035		 Sucrose-limitation induced kill switch Composite Coding region of Sucrose-limitation induced kill switch Characterize in Bacillus Subtilus