Difference between revisions of "Team:Lambert GA/Collaborations"

 
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Notebook">Notebook</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Notebook">Notebook</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/InterLab">InterLab</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/InterLab">InterLab</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Contribution">Contribution</a>
 
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a>
 
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Results">Results</a><a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Demonstrate">Demonstrate</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Results">Results</a><a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Demonstrate">Demonstrate</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Attributions">Attributions</a>
 
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Part Collection">Part Collection</a>
 
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<center> <h1 id="MainTitle"><b> Collaborations </b></h1>
 
<center> <h1 id="MainTitle"><b> Collaborations </b></h1>
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<center><p style="font-size: 20px; color: white; margin-left: 220px;">For more information, see our <a style="color:#D49AE6;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver Human Practices page</a>.</p>
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<h2 style="text-align:center"> Styczynski Lab at Georgia Tech </h2>
 
  
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<h2 style="text-align:center" color: #D49AE6>Gibson Ridge Software </h2>
 
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<i style="font-size: 14px;">Mr. Gibson meeting with our hardware committee.</i>  
 
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Mr. Gibson, the CEO and Founder of Gibson Ridge Software, worked with the Lambert High School iGEM team, specifically with the Hardware/Software committee to provide advice and feedback on the Chrome-Q. He met with the team during each iteration of the design and helped troubleshoot the electronic circuit. He also gave direction and feedback for developing the software.
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Our iGEM team was inspired for our project by the work in the Styczynski Lab at the Georgia Institute of Technology. Professor Styczynski and his graduate students, Monica McNerney and Daniel Watstein are currently working on engineering a zinc biosensor.  (Watstein, McNerney and Styczynski 2015) Their use of the violacein and carotenoid pathway as a biosensor for zinc micronutrient deficiencies led us to investigate other visual detection sensors.  Chromoproteins from the Uppsala 2013 collection seemed to be a natural place to start.  The Endy Lab and Edinburgh's 2014 projects into "degrons" or degradation tags were also inspirations for our project.
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In addition, Dr. Styczynski and Monica McNerney have been mentors and advisors to us. They have met with us multiple times to revise our project and troubleshoot issues both in person and by Skype calls. Dr. Styczynski has also generously provided materials that a high school lab does not have access to, and has periodically allowed us use of his lab for training and use of specialized equipment. We could not have completed our project without this collaboration!
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<h2 style="text-align:center" color: #D49AE6 > University of Georgia </h2>
 
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<h2 style="text-align:center" color: #D49AE6 > Centers for Disease Control (CDC) </h2>
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<i style="font-size: 14px;">Gaurav working with the plate reader at UGA.</i>  
 
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In order to further understand the impact of our project, we discussed micronutrient deficiencies with Dr. Ralph Whitehead, a researcher from the CDC. During the interview we asked questions about micronutrient
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By collaborating with the University of Georgia’s iGEM team, Lambert iGEM was able to complete the interlab measurement experiments. These were a series of detailed protocols involving the use of a plate reader to ensure accuracy within the fluorescence data. This allowed both teams to compare their data and openly discuss any unusual results that could have caused issues in the data analysis and interpretation. Specific errors were discovered and both teams were able to improve upon the technique demonstrating inter-laboratory studies to create a similar, comparable method of GFP measurement.
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deficiencies in multiple regions around the globe. The insightful answers gave us a better understanding of the micronutrient problems that affect different areas. Micronutrient deficiencies are often unspecified through different regions because micronutrient amounts available in the soil can vary between agricultural fields within the same village. He shared the specific process of blood analysis for micronutrient amounts. He also named other organizations that are working on the development of a household indicator for blood analysis. The process from blood sampling to results and actions to resolve the deficiencies can take weeks and has enormous resource costs. The blood samples must stay refrigerated and travel long distances to clinics or labs. The results of the tests then needs to be delivered back to the patient and caretakers. Dr. Whitehead also touched on the CDC’s specific process for blood analysis in targeted areas and was interested in micronutrient biosensors as a promising solution to an ever growing problem.
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<h2 style="text-align:center" color: #D49AE6 > New England Biolabs Representative : Chris Cook </h2>
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<h2 style="text-align:center" color: #D49AE6 > TAS Taipei </h2>
 
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<i style="font-size: 14px;">Lambert iGEM team in a conference call with TAS Taipei</i>  
 
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In the spring, a representative from New England Biolabs, Chris Cook, came to our school to discuss restriction enzymes and other products that we use in our lab.  The presentation was helpful not only on the scientific side, but also because he discussed career opportunities beyond a research lab.    
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Lambert iGEM sent Chrome-Q design files to TAS Taipei early in the design process.  A video conference call was held and subsequent communications took place to offer help and receive feedback. Specifically TAS Taipei gave Lambert some tips on integrating human practices. Lambert was able to share information about working in underfunded labs.  
 
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<h2 style="text-align:center" color: #D49AE6 > Alverno California and CAPS_Kansas </h2>
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<h2 style="text-align:center" color: #D49AE6 > Emory iGEM Team: Ethics Collaboration </h2>
 
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<i style="font-size: 14px;">Lambert iGEM team with the Emory iGEM team</i>  
 
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Our team collaborated with Alverno Heights Academy, a fellow high school iGEM team in California, and the CAPS Kansas iGEM Team. We set up a video call with them and discussed our project as well as helped them with understanding the iGEM Jamboree, and emailed multiple times back and forth, answering questions about the process of competing in iGEM and various requirements for Jamboree.
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The Lambert iGEM team was able to collaborate with the Emory iGEM team to discuss the ethics about CRISPR Cas9 along with a group of other Emory students and professors. Through this discussion, they were able to gain knowledge about the different perspectives of genetic modification and to what extent people are willing to justify experiments as ethical. Also, the Emory iGEM team led a tour of their labs to demonstrate their daily lab workings, allowing both teams to grasp the overall purpose of each other’s projects.  Lambert used this ethics conference as a springboard for their own investigations into ethics.  As a result the team developed a simple flowchart for the synthetic biologists to use when considering the ethics of their own projects. Members of the team also attended a Business Ethics Experience held by the University of North Georgia.
 
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<h2 style="text-align:center" color: #D49AE6 > Georgia State University </h2>
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<h2 style="text-align:center" color: #D49AE6 > Lambert High School: Engineering and MSA </h2>
 
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We collaborated with the Georgia State iGEM team in order to host our booth at the Maker Faire Atlanta. Members of GSU iGEM volunteered at the event and discussed their project with us during the breaks. They also generously donated materials we needed for our activities and were greatly helpful. We could not have hosted our booth without them!
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The Chrome-Q prototypes designed by the team were 3-D printed by the Lambert High School Engineering department. The final Chrome-Q prototype was ordered from Shapeways.  The engineering department printed the 3-D fuge designs obtained from the Prakash Lab from their Paperfuge project. An engineering student at Lambert High School, Sahil Jain, modified one of the 3-D fuge designs to improve ease of usability. This final model was used to process cells for the final data collection.
 
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<h2 style="text-align:center" color: #D49AE6 > TAS Taipei </h2>
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<h2 style="text-align:center" color: #D49AE6 > iGEM Twitter Group </h2>
 
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Description
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Thanks to Twitter, iGEM teams from all over the world are in a group chat where they can easily communicate and ask questions about iGEM, Jamboree, wiki, and much more! iGEM teams have easy access to other teams and can ask questions about their projects, send out surveys, or promote their apps/programs through this platform. A great and fun way to collaborate!
 
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<h2 style="text-align:center" color: #D49AE6 > Lambert High School: Engineering and MSA </h2>
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<h2 style="text-align:center" color: #D49AE6 >Chrome-Q</h2>
 
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In order to make our lightbox device, we collaborated with the engineering department at our school. A member of that department, David Park, helped adjust our existing blueprints and allowed us to use his 3-D printer. In addition, David also helped troubleshoot when we had problems assembling the lightbox and gave us ideas to improve future versions of it. We also collaborated with MSA, Lambert's very own Medical Science Academy, who helped us with events and volunteered for set-up and clean-up for our outreach.
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Lambert iGEM will continue to use the Chrome-Q instead of an expensive plate readers. Regardless of the project, the team will use chromoproteins as a reporter of the genetic sequences. As Lambert iGEM develops future projects, the team will improve the structure of the Chrome-Q. To add, the software will be further developed to be able to distinguish more color variations. To summarize, Lambert iGEM will expand the use of the Chrome-Q and app to accommodate the future projects.  
 
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<h2 style="text-align:center" color: #D49AE6 >Future Collaborations</h2>
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Thanks to Twitter, iGEM teams from all over the world are in a group chat where we can easily communicate and ask questions about iGEM, Jamboree, wiki, and much more! iGEM teams have easy access to other teams and can ask questions about their projects, send out surveys, or promote their apps/programs through this platform. A great and fun way to collaborate!
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The Lambert iGEM will continue to improve the 2017 project. They will collaborate with microbiologists at Emory and CDC, so the team can improve the genetic constructs that were not able to function. To add, they will continue to talk with Gibson Ridge Software to finish the app. Lastly, it is a goal of the Lambert iGEM team to send out the Chrome-Q to numerous labs. Dr. Dana Boyd Barr has shown interest in using the hardware for her labs in Thailand, Rwanda, and Peru.
 
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Latest revision as of 14:18, 15 December 2017


Collaborations


For more information, see our Silver Human Practices page.



Gibson Ridge Software




Mr. Gibson meeting with our hardware committee.
Mr. Gibson, the CEO and Founder of Gibson Ridge Software, worked with the Lambert High School iGEM team, specifically with the Hardware/Software committee to provide advice and feedback on the Chrome-Q. He met with the team during each iteration of the design and helped troubleshoot the electronic circuit. He also gave direction and feedback for developing the software.





University of Georgia




Gaurav working with the plate reader at UGA.
By collaborating with the University of Georgia’s iGEM team, Lambert iGEM was able to complete the interlab measurement experiments. These were a series of detailed protocols involving the use of a plate reader to ensure accuracy within the fluorescence data. This allowed both teams to compare their data and openly discuss any unusual results that could have caused issues in the data analysis and interpretation. Specific errors were discovered and both teams were able to improve upon the technique demonstrating inter-laboratory studies to create a similar, comparable method of GFP measurement.




TAS Taipei




Lambert iGEM team in a conference call with TAS Taipei
Lambert iGEM sent Chrome-Q design files to TAS Taipei early in the design process. A video conference call was held and subsequent communications took place to offer help and receive feedback. Specifically TAS Taipei gave Lambert some tips on integrating human practices. Lambert was able to share information about working in underfunded labs.















Emory iGEM Team: Ethics Collaboration




Lambert iGEM team with the Emory iGEM team
The Lambert iGEM team was able to collaborate with the Emory iGEM team to discuss the ethics about CRISPR Cas9 along with a group of other Emory students and professors. Through this discussion, they were able to gain knowledge about the different perspectives of genetic modification and to what extent people are willing to justify experiments as ethical. Also, the Emory iGEM team led a tour of their labs to demonstrate their daily lab workings, allowing both teams to grasp the overall purpose of each other’s projects. Lambert used this ethics conference as a springboard for their own investigations into ethics. As a result the team developed a simple flowchart for the synthetic biologists to use when considering the ethics of their own projects. Members of the team also attended a Business Ethics Experience held by the University of North Georgia.




Lambert High School: Engineering and MSA



The Chrome-Q prototypes designed by the team were 3-D printed by the Lambert High School Engineering department. The final Chrome-Q prototype was ordered from Shapeways. The engineering department printed the 3-D fuge designs obtained from the Prakash Lab from their Paperfuge project. An engineering student at Lambert High School, Sahil Jain, modified one of the 3-D fuge designs to improve ease of usability. This final model was used to process cells for the final data collection.





iGEM Twitter Group



Thanks to Twitter, iGEM teams from all over the world are in a group chat where they can easily communicate and ask questions about iGEM, Jamboree, wiki, and much more! iGEM teams have easy access to other teams and can ask questions about their projects, send out surveys, or promote their apps/programs through this platform. A great and fun way to collaborate!






Chrome-Q



Lambert iGEM will continue to use the Chrome-Q instead of an expensive plate readers. Regardless of the project, the team will use chromoproteins as a reporter of the genetic sequences. As Lambert iGEM develops future projects, the team will improve the structure of the Chrome-Q. To add, the software will be further developed to be able to distinguish more color variations. To summarize, Lambert iGEM will expand the use of the Chrome-Q and app to accommodate the future projects.





Future Collaborations


The Lambert iGEM will continue to improve the 2017 project. They will collaborate with microbiologists at Emory and CDC, so the team can improve the genetic constructs that were not able to function. To add, they will continue to talk with Gibson Ridge Software to finish the app. Lastly, it is a goal of the Lambert iGEM team to send out the Chrome-Q to numerous labs. Dr. Dana Boyd Barr has shown interest in using the hardware for her labs in Thailand, Rwanda, and Peru.