Difference between revisions of "Team:Lambert GA/Collaborations"

 
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Notebook">Notebook</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Notebook">Notebook</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/InterLab">InterLab</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/InterLab">InterLab</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Contribution">Contribution</a>
 
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Results">Results</a><a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Demonstrate">Demonstrate</a>
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Results">Results</a><a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Demonstrate">Demonstrate</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Improve">Improve</a>
 
 
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Attributions">Attributions</a>
 
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Part Collection">Part Collection</a>
 
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       <a href="https://2017.igem.org/Team:Lambert_GA/HP/Silver" class="dropbtn">Human Practices</a>
 
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Human_Practices">Human Practices</a>
 
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a>
 
       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Gold_Integrated">Integrated and Gold</a>
 
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Engagement">Public Engagement</a>
 
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       <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Applied_Design">Applied Design</a>
 
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Software">Software</a>
 
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<center> <h1 id="MainTitle"><b> Collaborations </b></h1>
 
<center> <h1 id="MainTitle"><b> Collaborations </b></h1>
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<center><p style="font-size: 20px; color: white; margin-left: 220px;">For more information, see our <a style="color:#D49AE6;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver Human Practices page</a>.</p>
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<h2 style="text-align:center"> Styczynski Lab at Georgia Tech </h2>
 
  
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<h2 style="text-align:center" color: #D49AE6>Gibson Ridge Software </h2>
 
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<i style="font-size: 14px;">Mr. Gibson meeting with our hardware committee.</i>  
 
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Mr. Gibson, the CEO and Founder of Gibson Ridge Software, worked with the Lambert High School iGEM team, specifically with the Hardware/Software committee to provide advice and feedback on the Chrome-Q. He met with the team during each iteration of the design and helped troubleshoot the electronic circuit. He also gave direction and feedback for developing the software.
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Our iGEM team was inspired for our project by the work in the Styczynski Lab at the Georgia Institute of Technology. Professor Styczynski and his graduate students, Monica McNerney and Daniel Watstein are currently working on engineering a zinc biosensor.  (Watstein, McNerney and Styczynski 2015) Their use of the violacein and carotenoid pathway as a biosensor for zinc micronutrient deficiencies led us to investigate other visual detection sensors.  Chromoproteins from the Uppsala 2013 collection seemed to be a natural place to start.  The Endy Lab and Edinburgh's 2014 projects into "degrons" or degradation tags were also inspirations for our project.
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In addition, Dr. Styczynski and Monica McNerney have been mentors and advisors to us. They have met with us multiple times to revise our project and troubleshoot issues both in person and by Skype calls. Dr. Styczynski has also generously provided materials that a high school lab does not have access to, and has periodically allowed us use of his lab for training and use of specialized equipment. We could not have completed our project without this collaboration!
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<h2 style="text-align:center" color: #D49AE6 > University of Georgia </h2>
 
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<h2 style="text-align:center" color: #D49AE6 > Open House </h2>
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<i style="font-size: 14px;">Gaurav working with the plate reader at UGA.</i>  
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Lambert iGEM introduced their 2017 project to companies, parents, and students in our community. Individuals were able to conduct lab protocols and work on lab techniques such as gel electrophoresis and micropipetting. Furthermore, Lambert iGEM held a banquet for Lambert iGEM graduates.  
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By collaborating with the University of Georgia’s iGEM team, Lambert iGEM was able to complete the interlab measurement experiments. These were a series of detailed protocols involving the use of a plate reader to ensure accuracy within the fluorescence data. This allowed both teams to compare their data and openly discuss any unusual results that could have caused issues in the data analysis and interpretation. Specific errors were discovered and both teams were able to improve upon the technique demonstrating inter-laboratory studies to create a similar, comparable method of GFP measurement.
 
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<h2 style="text-align:center" color: #D49AE6 >Gibson Ridge Software </h2>
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<h2 style="text-align:center" color: #D49AE6 > TAS Taipei </h2>
 
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<i style="font-size: 14px;">Lambert iGEM team in a conference call with TAS Taipei</i>  
 
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Mr. Gibson, the CEO and Founder of Gibson Ridge Software, came to Lambert High School and met with the Hardware/Software community to provide advice and give feedback on the current state of our prototype, the DomeQ. He also gave recommendations on the software side of the DomeQ.
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Lambert iGEM sent Chrome-Q design files to TAS Taipei early in the design process. A video conference call was held and subsequent communications took place to offer help and receive feedback. Specifically TAS Taipei gave Lambert some tips on integrating human practices. Lambert was able to share information about working in underfunded labs.  
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<h2 style="text-align:center" color: #D49AE6 > University of Georgia </h2>
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Lambert iGEM collaborated with Dr. Karls, a microbiology professor at the University of Georgia. She gave feedback about how to assemble our construct more effectively.
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<h2 style="text-align:center" color: #D49AE6 > Emory iGEM Team: Ethics Collaboration </h2>
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<h2 style="text-align:center" color: #D49AE6 > TAS Taipei </h2>
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<i style="font-size: 14px;">Lambert iGEM team with the Emory iGEM team</i>  
 
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Description
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The Lambert iGEM team was able to collaborate with the Emory iGEM team to discuss the ethics about CRISPR Cas9 along with a group of other Emory students and professors. Through this discussion, they were able to gain knowledge about the different perspectives of genetic modification and to what extent people are willing to justify experiments as ethical. Also, the Emory iGEM team led a tour of their labs to demonstrate their daily lab workings, allowing both teams to grasp the overall purpose of each other’s projects.  Lambert used this ethics conference as a springboard for their own investigations into ethics.  As a result the team developed a simple flowchart for the synthetic biologists to use when considering the ethics of their own projects. Members of the team also attended a Business Ethics Experience held by the University of North Georgia. 
 
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<h2 style="text-align:center" color: #D49AE6 > Lambert High School: Engineering and MSA </h2>
 
<h2 style="text-align:center" color: #D49AE6 > Lambert High School: Engineering and MSA </h2>
 
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In order to make our DomeQ, we collaborated with the engineering department at our school. A student, Sahil Jain, printed the early prototypes of the DomeQ. The engineering department allowed us to use their 3-D printer, and print our current prototype. In addition, they were also able to print out wheels for Paperfuge&#9400;. We also collaborated with MSA, Lambert's very own Medical Science Academy, who helped us with events and volunteered for set-up and clean-up for our outreach.
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The Chrome-Q prototypes designed by the team were 3-D printed by the Lambert High School Engineering department. The final Chrome-Q prototype was ordered from Shapeways.  The engineering department printed the 3-D fuge designs obtained from the Prakash Lab from their Paperfuge project. An engineering student at Lambert High School, Sahil Jain, modified one of the 3-D fuge designs to improve ease of usability. This final model was used to process cells for the final data collection.
 
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<h2 style="text-align:center" color: #D49AE6 > iGEM Twitter Group </h2>
 
<h2 style="text-align:center" color: #D49AE6 > iGEM Twitter Group </h2>
 
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Thanks to Twitter, iGEM teams from all over the world are in a group chat where we can easily communicate and ask questions about iGEM, Jamboree, wiki, and much more! iGEM teams have easy access to other teams and can ask questions about their projects, send out surveys, or promote their apps/programs through this platform. A great and fun way to collaborate!
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Thanks to Twitter, iGEM teams from all over the world are in a group chat where they can easily communicate and ask questions about iGEM, Jamboree, wiki, and much more! iGEM teams have easy access to other teams and can ask questions about their projects, send out surveys, or promote their apps/programs through this platform. A great and fun way to collaborate!
 
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<h2 style="text-align:center" color: #D49AE6 > Lambert High School: Engineering and MSA </h2>
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<h2 style="text-align:center" color: #D49AE6 >Chrome-Q</h2>
 
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In order to make our DomeQ, we collaborated with the engineering department at our school. A student, Sahil Jain, printed the early prototypes of the DomeQ. The engineering department allowed us to use their 3-D printer, and print our current prototype. In addition, they were also able to print out wheels for Paperfuge&#9400;. We also collaborated with MSA, Lambert's very own Medical Science Academy, who helped us with events and volunteered for set-up and clean-up for our outreach.
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Lambert iGEM will continue to use the Chrome-Q instead of an expensive plate readers. Regardless of the project, the team will use chromoproteins as a reporter of the genetic sequences. As Lambert iGEM develops future projects, the team will improve the structure of the Chrome-Q. To add, the software will be further developed to be able to distinguish more color variations. To summarize, Lambert iGEM will expand the use of the Chrome-Q and app to accommodate the future projects.  
 
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<h2 style="text-align:center" color: #D49AE6 > iGEM Twitter Group </h2>
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<h2 style="text-align:center" color: #D49AE6 >Future Collaborations</h2>
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Latest revision as of 14:18, 15 December 2017


Collaborations


For more information, see our Silver Human Practices page.



Gibson Ridge Software




Mr. Gibson meeting with our hardware committee.
Mr. Gibson, the CEO and Founder of Gibson Ridge Software, worked with the Lambert High School iGEM team, specifically with the Hardware/Software committee to provide advice and feedback on the Chrome-Q. He met with the team during each iteration of the design and helped troubleshoot the electronic circuit. He also gave direction and feedback for developing the software.





University of Georgia




Gaurav working with the plate reader at UGA.
By collaborating with the University of Georgia’s iGEM team, Lambert iGEM was able to complete the interlab measurement experiments. These were a series of detailed protocols involving the use of a plate reader to ensure accuracy within the fluorescence data. This allowed both teams to compare their data and openly discuss any unusual results that could have caused issues in the data analysis and interpretation. Specific errors were discovered and both teams were able to improve upon the technique demonstrating inter-laboratory studies to create a similar, comparable method of GFP measurement.




TAS Taipei




Lambert iGEM team in a conference call with TAS Taipei
Lambert iGEM sent Chrome-Q design files to TAS Taipei early in the design process. A video conference call was held and subsequent communications took place to offer help and receive feedback. Specifically TAS Taipei gave Lambert some tips on integrating human practices. Lambert was able to share information about working in underfunded labs.















Emory iGEM Team: Ethics Collaboration




Lambert iGEM team with the Emory iGEM team
The Lambert iGEM team was able to collaborate with the Emory iGEM team to discuss the ethics about CRISPR Cas9 along with a group of other Emory students and professors. Through this discussion, they were able to gain knowledge about the different perspectives of genetic modification and to what extent people are willing to justify experiments as ethical. Also, the Emory iGEM team led a tour of their labs to demonstrate their daily lab workings, allowing both teams to grasp the overall purpose of each other’s projects. Lambert used this ethics conference as a springboard for their own investigations into ethics. As a result the team developed a simple flowchart for the synthetic biologists to use when considering the ethics of their own projects. Members of the team also attended a Business Ethics Experience held by the University of North Georgia.




Lambert High School: Engineering and MSA



The Chrome-Q prototypes designed by the team were 3-D printed by the Lambert High School Engineering department. The final Chrome-Q prototype was ordered from Shapeways. The engineering department printed the 3-D fuge designs obtained from the Prakash Lab from their Paperfuge project. An engineering student at Lambert High School, Sahil Jain, modified one of the 3-D fuge designs to improve ease of usability. This final model was used to process cells for the final data collection.





iGEM Twitter Group



Thanks to Twitter, iGEM teams from all over the world are in a group chat where they can easily communicate and ask questions about iGEM, Jamboree, wiki, and much more! iGEM teams have easy access to other teams and can ask questions about their projects, send out surveys, or promote their apps/programs through this platform. A great and fun way to collaborate!






Chrome-Q



Lambert iGEM will continue to use the Chrome-Q instead of an expensive plate readers. Regardless of the project, the team will use chromoproteins as a reporter of the genetic sequences. As Lambert iGEM develops future projects, the team will improve the structure of the Chrome-Q. To add, the software will be further developed to be able to distinguish more color variations. To summarize, Lambert iGEM will expand the use of the Chrome-Q and app to accommodate the future projects.





Future Collaborations


The Lambert iGEM will continue to improve the 2017 project. They will collaborate with microbiologists at Emory and CDC, so the team can improve the genetic constructs that were not able to function. To add, they will continue to talk with Gibson Ridge Software to finish the app. Lastly, it is a goal of the Lambert iGEM team to send out the Chrome-Q to numerous labs. Dr. Dana Boyd Barr has shown interest in using the hardware for her labs in Thailand, Rwanda, and Peru.