Difference between revisions of "Team:Kobe/HP/Gold Integrated"

 
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<img src=" https://static.igem.org/mediawiki/2017/4/4d/T--Kobe--temomi.png " height="200"/>
 
<img src=" https://static.igem.org/mediawiki/2017/4/4d/T--Kobe--temomi.png " height="200"/>
 
</div>
 
</div>
−
 
+
<h1>Visit to tea farm</h1>
−
 
+
<p>For our Human Practice project, we hope to help promoting Japanese tea with our biosensor. Our tea-quality theanine sensor should be useful when our project reflects the actual situation of tea industry. The best way to learn and better understand the current situation of Japanese tea world is, literally to get out of the lab and experience it with our hearts!</p>
 
     <h3>
 
     <h3>
−
         <br/>Interview with Mr.Matsumoto: Master of farm work in “Teppen”
+
         <br/><br/><br/>Interview with Mr.Matsumoto: The owner of a farm house, “Teppen”
 
     </h3>
 
     </h3>
 
     <p>
 
     <p>
 
         8 Oct. 2017
 
         8 Oct. 2017
 
<br />
 
<br />
−
  <br />      We visited the facility named “Teppen” which is a guest house and a farm.
+
  <br />      We visited a tea farm house, “Teppen” in Ehime prefecture.
 
         <br />
 
         <br />
−
       <br /> <a href="https://teppen-stay.jimdo.com/%E3%81%A6%E3%81%A3%E3%81%BA%E3%82%93%E7%B4%85%E8%8C%B6/">The link of the inn</a>  
+
       <br /> <a href="https://teppen-stay.jimdo.com/">The link of the farm house</a>  
 
         <br />
 
         <br />
−
   <br />     “Yabukita”, a type of tea is cultivated there. It usually used in green tea.
+
   <br />     They cultivate “Yabukita”, a type of tea plant usually used for green tea. We heard a lot of interesting and useful information related to our project. <br/><br/>Below are the topics we heard.  
−
  <br />    I learned interesting and useful information about our study from Mr.Matsumoto, his wife and the worker.
+
−
  <br />     Below are the itemized information that I have gathered.
+
  
 
     </p>
 
     </p>
−
     <h4>
+
     <h4><br/>
−
         ○Most of the currently marketed green tea contains the additive
+
         ○ Some of the marketed green tea contains additives.
 
     </h4>
 
     </h4>
  
 
     <p>
 
     <p>
−
         They sometimes compare the tastes of marketed tea.
+
         They sometimes tastes marketed tea pruduct.
−
         Cheap or imported one often contain the additive to enhance the taste of the tea.
+
         Cheap or imported ones often contain additives to enhance the taste of the tea.
  
 
     </p>
 
     </p>
 
     <p>
 
     <p>
−
         The farmers determined if the tea has additive or not by putting it overnight. If there are a lot of additives, it will turn into a milky color.
+
         Mr.Matsumoto said he can learn whether additives are contained in the tea or not by putting it overnight. If they contain lots of additives, the tea will turn into milky color.
 
     </p>
 
     </p>
  
 
     <p>
 
     <p>
−
         According to the following references, adding sodium glutamate into green tea have been undertaken from Sanin Region through into the Hokuriku region in Japan.
+
         According to the following references, the custom of adding monosodium glutamate (MSG) into green tea is widely seen from Sanin Region through into the Hokuriku region in Japan.<br/>
−
         <br />後藤 哲久・堀江 秀樹・向井 俊博（2009），添加茶の化学成分による判別法（Japanese）
+
         <br /><a href="https://www.jstage.jst.go.jp/article/cha1953/1992/76/1992_76_33/_pdf">添加茶の化学成分による判別法</a>（Japanese)
 
     </p>
 
     </p>
 
     <p>
 
     <p>
−
         This type of green tea can be determined by the ratio of the content of glutamic acid to the content of Theanine.
+
         The amount of additives in tea can be determined by the ratio of glutamate content to the theanine content.
 
     </p>
 
     </p>
 
     <p>
 
     <p>
−
         In the case of tea additive-free, the ratio of the content of glutamic acid to the content of Theanine is 0.2 or less. However, the ratio is sometimes more than 0.4 in cases where the tea is containing the additive.
+
         In the case of natural tea (without additives), the value of this ratio is about 0.2 or less. However, this ratio sometimes exceeds more than 0.4 in cases where the tea is containing the additive. Glutamate is often added to enhance umami in Japanese green tea, but that taste is quite different from the original one(umami of theanine). Our theanine sensor can be used to detect the additives in tea.
 
     </p>
 
     </p>
  
−
     <p>
+
     <h4><br/>
−
        The taste of glutamic acid which is much cheaper than Theanine is completely different from the original taste of Japanese green tea.
+
         ○The taste of green tea is affected by many factors.
−
        Our Theanine sensor can be used to differentiate the tea containing the additive.
+
−
    </p>
+
−
 
+
−
    <h4>
+
−
         ○The taste of green tea changed for some reasons despite the same cultivation method.
+
 
     </h4>
 
     </h4>
 
     <p>
 
     <p>
−
         There are many factors to consider that affect the change of the taste of the tea despite the same cultivation method.
+
         There are many factors that could change the taste of tea. The taste of tea is variable even if it is cultivated in the same way.
−
         For example, the slight difference of place like facing across the road, the degree of damage done by insects and the different way of how people processed the tea.
+
         For example, the factors include the places they are planted, the degree of damage caused by insects, and the way of processing the tea.
  
 
     </p>
 
     </p>
−
     <p>
+
     <p>  
−
         Only large facilities have the device to measure the component analysis so it is usually measured all at once. However, they said that these slight differences which described above are important factors to evaluate the quality of tea. We recognized the importance of inexpensive sensor.
+
         Only the large scale facilities own the devices to analyze the components in tea because such devices are expensive. Small farms cannot afford them so they cannot check their tea quality when they want. We realized again the importance of synthetic biological tool to measure the tea quality.
 
     </p>
 
     </p>
  
−
     <h4>
+
     <h4><br/>
 
         ○Decrease in consumption of green tea
 
         ○Decrease in consumption of green tea
 
     </h4>
 
     </h4>
  
 
     <p>
 
     <p>
−
         According to the following references, the amount of consumption of green tea in Japan is decreasing these days.<br />
+
         According to the following references, the consumption of green tea in Japan is decreasing these days.<br />
 
         <br /><a href="http://www.zennoh.or.jp/bu/nousan/tea/seisan01b.htm">茶類の国内消費量の推移</a>（Japanese）
 
         <br /><a href="http://www.zennoh.or.jp/bu/nousan/tea/seisan01b.htm">茶類の国内消費量の推移</a>（Japanese）
 
     </p>
 
     </p>
 
     <p>
 
     <p>
−
         Indeed, farmers stated about the decrease of sales of green tea.
+
         Indeed, the tea farmers also mentioned the decrease in green tea sales. Our "theanine sensor" would help promoting Japanese tea.
−
    </p>
+
Natural green tea contains a lot of L-theanine. Our sensor will prove that!
−
    <p>
+
−
        To educate the culture of Japanese green tea is important, but to make Japanese understand the good benefits brought by natural green tea is also important.    </p>
+
−
    <p>
+
−
        Our Theanine sensor might help the appeal of it because Japanese people nowadays are attracted to beverages that promises good healthy benefits but these beverages are added by Theanine artificially.
+
−
 
+
−
In green tea, there are a lot of Theanine which is naturally contained. Our sensor will make the fact known to the public.
+
−
 
+
 
     </p>
 
     </p>
 
      
 
      
 
         <p>
 
         <p>
−
         After our interview, we experienced tea-picking and hand-rolling.
+
         After the interview, the owner generously allowed us to experience tea-picking and hand-rolling.
−
         <br />(Hand rolling is the primary process of making green tea)
+
         (Hand rolling is the primary process of making green tea)
  
 
     </p>
 
     </p>
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       <img src="https://static.igem.org/mediawiki/2017/c/c1/T--Kobe--HP10.jpg"/>
 
       <img src="https://static.igem.org/mediawiki/2017/c/c1/T--Kobe--HP10.jpg"/>
 
       <figcaption>
 
       <figcaption>
−
           Tea leaves are heated to make it softer.
+
           Tea leaves were heated to be softened.
 
       </figcaption>
 
       </figcaption>
 
   </figure>
 
   </figure>
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         <img src="https://static.igem.org/mediawiki/2017/8/88/T--Kobe--HP11.jpg"/>
 
         <img src="https://static.igem.org/mediawiki/2017/8/88/T--Kobe--HP11.jpg"/>
 
         <figcaption>
 
         <figcaption>
−
             Hand-rolling to extract water from tea leaves
+
             Hand-rolling to dry tea leaves
 
         </figcaption>
 
         </figcaption>
 
     </figure>
 
     </figure>
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         <img src="https://static.igem.org/mediawiki/2017/a/a0/T--Kobe--HP12.jpg"/>
 
         <img src="https://static.igem.org/mediawiki/2017/a/a0/T--Kobe--HP12.jpg"/>
 
         <figcaption>
 
         <figcaption>
−
             Green tea have been dried in the sun to preserve it for a long time.
+
             Green tea was exposed in the sun. Dried tea leaves can be preserved for a long time.
 
         </figcaption>
 
         </figcaption>
 
     </figure>
 
     </figure>
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         <img src="https://static.igem.org/mediawiki/2017/0/07/T--Kobe--HP14.jpg" />
 
         <img src="https://static.igem.org/mediawiki/2017/0/07/T--Kobe--HP14.jpg" />
 
         <figcaption>
 
         <figcaption>
−
             We have consulted them about our pamphlets for green tea
+
             They helped us create our booklets.</figcaption>
−
        </figcaption>
+
 
     </figure>
 
     </figure>
 
</br>
 
</br>
  
 
     <h3>
 
     <h3>
−
         Interview with the representative of cultivation of Benifuuki group  
+
         <br/><br/><br/><br/>Interview with the representative of Benifuuki group  
 
     </h3>
 
     </h3>
 
     <p>
 
     <p>
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     <img src="https://static.igem.org/mediawiki/2017/7/77/T--Kobe--HP15.jpg" />
 
     <img src="https://static.igem.org/mediawiki/2017/7/77/T--Kobe--HP15.jpg" />
 
     <p>
 
     <p>
−
         We interviewed with the representative of cultivation of Benifuuki group.  
+
         We interviewed the representative of Benifuuki group.  
 
     </p>
 
     </p>
 
     <p>
 
     <p>
−
         “Benifuuki” is a type of tea which is commonly used in black tea. But these days, it is also used in green tea because it contains a lot of Catechin which suppress the symptoms accompanying the allergy to pollen.
+
         “Benifuuki” is a type of tea commonly used for black tea. But these days, it is also used for green tea because of its high content of catechin, the substance which suppresses the symptoms of hay fever.
−
         I learned interesting and useful information about our study from him.
+
         We heard interesting stories there.
−
<br />     Below are the itemized information that I have gathered.
+
<br /><br />        </p>
  
−
    </p>
+
          
−
 
+
<h4><br/>
−
    <h4>
+
         The necessity of small device to analyze  the components of tea accurately
−
         ○Lack of manpower due to aging
+
−
    </h4>
+
−
    <p>
+
−
        There are many tea plantations which have been left neglected for years.
+
−
These days, they change their orange farms into tea plantation because tea is easier to grow. So they grow “Benifuuki” which is new breed and attracts people’s attention by suppressing the symptoms of hay fever.
+
−
            If the market of health tea will increase, the labor shortage will also be solved little by little.
+
−
 
+
−
    </p>
+
−
    <figure>
+
−
         <img src="https://static.igem.org/mediawiki/2017/1/10/T--Kobe--HP16.jpg" />
+
−
        <figcaption>A tea plantation that is left to grow</figcaption>
+
−
    </figure>
+
−
   
+
−
<h4>
+
−
        ○The necessity of small device which can measure the components of tea accurately
+
 
</h4>
 
</h4>
  
 
<p>
 
<p>
  
−
                     To measure the component analysis in a way that is publicly certified is difficult on a small scale.
+
                     Analyzing the component of tea in a publicly certified way is difficult without costs.
−
            They said that the content of Catechin affect sales directly.
+
                      We believe that small biosensor will be able to measure the components of tea accurately and will be publicly certified.
−
            The green tea in Nakazima which we visited contains a lot of Cathchin but they cannot appeal about this because its small scale.
+
−
            We believe that small biosensor will be able to measure the components of tea accurately and will be publicly certified.
+
 
     </p>
 
     </p>
 
     <img src="https://static.igem.org/mediawiki/2017/c/cd/T--Kobe--HP17.jpg" />
 
     <img src="https://static.igem.org/mediawiki/2017/c/cd/T--Kobe--HP17.jpg" />
  
 +
    </br>
 +
<br> </br>
 +
    <h1><br/><br/><br/>
 +
        Survey on Tea Culture
 +
    </h1>
 
     <p>
 
     <p>
−
    ”Benifuuki” prefer warmer climate than “Yabukita” and have tolerance to sea breeze.</p>
+
      As a human practice project, we decided to help promoting Japanese tea to the world. We carried out a survey to collect useful data for promoting Japanese tea.  
−
</br>
+
          
−
 
+
−
    <h3>
+
−
        Survey on tea culture
+
−
    </h3>
+
−
    <p>
+
−
        The purpose of the survey is to compare data green tea culture among different regions.
+
−
         We ask them 9 questions.
+
 
     </p>
 
     </p>
 
     <img src="https://static.igem.org/mediawiki/2017/f/f6/T--Kobe--HP18.jpg" />
 
     <img src="https://static.igem.org/mediawiki/2017/f/f6/T--Kobe--HP18.jpg" />
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     </p>
 
     </p>
 
     <img src="https://static.igem.org/mediawiki/2017/a/a1/T--Kobe--HP19.jpg" />
 
     <img src="https://static.igem.org/mediawiki/2017/a/a1/T--Kobe--HP19.jpg" />
−
    <img src="https://static.igem.org/mediawiki/2017/1/15/T--Kobe--HP20.jpg" />
+
        <img src="https://static.igem.org/mediawiki/2017/1/15/T--Kobe--HP20.jpg" />
  
 
     <p>
 
     <p>
−
         As indicated in the chart, Japan has the greater number of people who enjoys tea.
+
         As indicated in the chart above, in Japan, there are greater number of people who enjoy tea.  
−
         For this data, enjoying the taste of tea has become a part of Japanese culture.
+
         For this data, we can say that enjoying the taste of tea has become an important part of Japanese culture.
 
     </p>
 
     </p>
 +
<img src="https://static.igem.org/mediawiki/2017/7/73/T--Kobe--HP23.jpg" />
 +
    <img src="https://static.igem.org/mediawiki/2017/2/27/T--Kobe--HP24.jpg" />
 +
    <p>
 +
        All participants who took the survey have already tasted green tea somewhere. Most of them already heard about  Japanese tea product.
 +
        Japanese tea seems to be more famous than we have thought.
 +
    </p>
 +
<img src="https://static.igem.org/mediawiki/2017/4/4d/T--Kobe--HP27.jpg"/>
 +
 
     <p>
 
     <p>
−
         Even though enjoying the taste of green tea is a Japanese culture, green tea doesn’t have big market share in Japan compared to other region.
+
         Even though green tea has been an important part of food culture in Japan, green tea does not have big market share in Japan compared to other region.
 
     </p>
 
     </p>
 
     <img src="https://static.igem.org/mediawiki/2017/9/93/T--Kobe--HP21.jpg" />
 
     <img src="https://static.igem.org/mediawiki/2017/9/93/T--Kobe--HP21.jpg" />
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     <p>
 
     <p>
−
         In all region, at least about 40% people think that health effect is a very important aspect when choosing tea and most of the people know that different type of tea have different effects on your health.
+
         In all regions, at least about 40% people think that health effect is a very important aspect when choosing tea. Most of them know that different type of tea have different effects on one's health.
−
         There are many people in the world who are interested in health effect of tea. The day that Theanine gets a lot of attention like Catechin is near.
+
         Many people are interested in health effects of tea. The day that theanine attract attention is near.
 
     </p>
 
     </p>
−
    <img src="https://static.igem.org/mediawiki/2017/7/73/T--Kobe--HP23.jpg" />
+
         <img src="https://static.igem.org/mediawiki/2017/3/39/T--Kobe--HP25.jpg" />
−
    <img src="https://static.igem.org/mediawiki/2017/2/27/T--Kobe--HP24.jpg" />
+
−
    <p>
+
−
         All the people who took the survey has already tasted the green tea and most of them know Japanese tea product.
+
−
        Japanese tea culture has higher name recognition than I thought.
+
−
    </p>
+
−
    <img src="https://static.igem.org/mediawiki/2017/3/39/T--Kobe--HP25.jpg" />
+
 
     <img src="https://static.igem.org/mediawiki/2017/2/28/T--Kobe--HP26.jpg" />
 
     <img src="https://static.igem.org/mediawiki/2017/2/28/T--Kobe--HP26.jpg" />
  
Line 214: Line 182:
  
 
     <p>
 
     <p>
−
         Profile of theanine in Japan was higher compared to other regions. (Except for category other regions in which there are only two people in this category and they know Theanine.)
+
         Theanine is more familiar in Japan than in other regions. (Except for category other regions in which there are only two people.)
−
         There are some people who pointed out that Japanese has a lot of interest in the healthy beverage in the free-answer column of the survey.
+
         Some people pointed out that Japanese has a lot of interest in the healthy beverage in the free-answer column of the survey.
−
         We agree with this opinion. They heard about theanine in various situations and was reported in a variety of media in Japan.
+
         We agree with this opinion.   </p>
−
    </p>
+
  
 
     <p>
 
     <p>
−
         Organization Chart
+
 
−
        <ul style="list-style-type: circle">
+
         <h5>Organization Chart</h5>
 +
</br>
 +
 
 +
            <ul style="list-style-type: circle">
 
             <li>Ehime Prefecture International Center<br /> Secretary‐general　Ryo Takaoka</li>
 
             <li>Ehime Prefecture International Center<br /> Secretary‐general　Ryo Takaoka</li>
 
             <li>Matsuyama International Center（MIC）　<br />Maki Mizuno</li>
 
             <li>Matsuyama International Center（MIC）　<br />Maki Mizuno</li>
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         }
 
         }
 
         img{
 
         img{
−
         max-width:100%
+
         max-width:100%;
 +
       
 
         }
 
         }
  

Latest revision as of 18:08, 15 December 2017


Integrated Human Practices

Visit to tea farm

For our Human Practice project, we hope to help promoting Japanese tea with our biosensor. Our tea-quality theanine sensor should be useful when our project reflects the actual situation of tea industry. The best way to learn and better understand the current situation of Japanese tea world is, literally to get out of the lab and experience it with our hearts!




Interview with Mr.Matsumoto: The owner of a farm house, “Teppen”

8 Oct. 2017

We visited a tea farm house, “Teppen” in Ehime prefecture.

The link of the farm house

They cultivate “Yabukita”, a type of tea plant usually used for green tea. We heard a lot of interesting and useful information related to our project.

Below are the topics we heard.


○ Some of the marketed green tea contains additives.

They sometimes tastes marketed tea pruduct. Cheap or imported ones often contain additives to enhance the taste of the tea.

Mr.Matsumoto said he can learn whether additives are contained in the tea or not by putting it overnight. If they contain lots of additives, the tea will turn into milky color.

According to the following references, the custom of adding monosodium glutamate (MSG) into green tea is widely seen from Sanin Region through into the Hokuriku region in Japan.

添加茶の化学成分による判別法（Japanese)

The amount of additives in tea can be determined by the ratio of glutamate content to the theanine content.

In the case of natural tea (without additives), the value of this ratio is about 0.2 or less. However, this ratio sometimes exceeds more than 0.4 in cases where the tea is containing the additive. Glutamate is often added to enhance umami in Japanese green tea, but that taste is quite different from the original one(umami of theanine). Our theanine sensor can be used to detect the additives in tea.


○The taste of green tea is affected by many factors.

There are many factors that could change the taste of tea. The taste of tea is variable even if it is cultivated in the same way. For example, the factors include the places they are planted, the degree of damage caused by insects, and the way of processing the tea.

Only the large scale facilities own the devices to analyze the components in tea because such devices are expensive. Small farms cannot afford them so they cannot check their tea quality when they want. We realized again the importance of synthetic biological tool to measure the tea quality.


○Decrease in consumption of green tea

According to the following references, the consumption of green tea in Japan is decreasing these days.

茶類の国内消費量の推移（Japanese）

Indeed, the tea farmers also mentioned the decrease in green tea sales. Our "theanine sensor" would help promoting Japanese tea. Natural green tea contains a lot of L-theanine. Our sensor will prove that!

After the interview, the owner generously allowed us to experience tea-picking and hand-rolling. (Hand rolling is the primary process of making green tea)

Tea-picking
Tea leaves were heated to be softened.
Hand-rolling to dry tea leaves
Green tea was exposed in the sun. Dried tea leaves can be preserved for a long time.
They helped us create our booklets.





Interview with the representative of Benifuuki group

9 Oct. 2017

We interviewed the representative of Benifuuki group.

“Benifuuki” is a type of tea commonly used for black tea. But these days, it is also used for green tea because of its high content of catechin, the substance which suppresses the symptoms of hay fever. We heard interesting stories there.


The necessity of small device to analyze the components of tea accurately

Analyzing the component of tea in a publicly certified way is difficult without costs. We believe that small biosensor will be able to measure the components of tea accurately and will be publicly certified.







Survey on Tea Culture

As a human practice project, we decided to help promoting Japanese tea to the world. We carried out a survey to collect useful data for promoting Japanese tea.

Percentage of individuals from different places who took the survey.

As indicated in the chart above, in Japan, there are greater number of people who enjoy tea. For this data, we can say that enjoying the taste of tea has become an important part of Japanese culture.

All participants who took the survey have already tasted green tea somewhere. Most of them already heard about Japanese tea product. Japanese tea seems to be more famous than we have thought.

Even though green tea has been an important part of food culture in Japan, green tea does not have big market share in Japan compared to other region.

In all regions, at least about 40% people think that health effect is a very important aspect when choosing tea. Most of them know that different type of tea have different effects on one's health. Many people are interested in health effects of tea. The day that theanine attract attention is near.

Theanine is more familiar in Japan than in other regions. (Except for category other regions in which there are only two people.) Some people pointed out that Japanese has a lot of interest in the healthy beverage in the free-answer column of the survey. We agree with this opinion.

Organization Chart

  • Ehime Prefecture International Center
    Secretary‐general　Ryo Takaoka
  • Matsuyama International Center（MIC）　
    Maki Mizuno
  • 愛媛県中予地方局商工観光室
    Miyuki Yamanaka
  • 愛媛県港湾管理事務所

Our sincere thanks to these people!