Difference between revisions of "Team:Oxford"

(Undo revision 507996 by Kmansatta (talk))
 
(85 intermediate revisions by 6 users not shown)
Line 5: Line 5:
 
<style>
 
<style>
 
canvas {
 
canvas {
−
    border:1px solid #d3d3d3;
 
 
     background-color: #e8d5d7;
 
     background-color: #e8d5d7;
−
     width:100%;
+
     width:80%;
 +
}
 +
#canvas_happy{
 +
    background-color: #002147;
 
}
 
}
  
−
</style>
+
hr {
−
</head>
+
    display: block;
−
<body onload="startStartScreen()">
+
    height: 1px;
 +
    border: 0;
 +
    border-top: 1px solid black;
 +
    margin: 1em 0;
 +
    padding: 0;
 +
}
  
−
 
+
.btn-circle {
−
<img id="magnify-glass" src="https://static.igem.org/mediawiki/2017/a/a6/T--oxford--manifying-glass.png" style = "display:none">
+
  width: 50px;
−
<img id="red-blood-cell" src = "https://static.igem.org/mediawiki/2017/d/d6/T--oxford--home-rbc.png" style = "display:none">
+
   height: 50px;
−
<img id="t-cruzi" src = "https://static.igem.org/mediawiki/2017/4/42/T--oxford--t_cruzi_cartoon.png" style = "display: none">
+
    padding: 10px 16px;
−
<img id="start_screen_img" src = "https://static.igem.org/mediawiki/2017/c/c8/T--oxford--home--background.jpeg" style = "display: none">
+
     border-radius: 50%;
−
 
+
     font-size: 24px;
−
<div class="container-fluid">
+
     line-height: 1.33;
−
<div id='header' style="margin-top: 100px"></div>
+
     background-color: rgba(95,155,175,0.9);
−
<h1 id='title' class="text-center"><i>See. cruzi</i></h1>    
+
−
<div id="wrapper" role="wrapper">
+
−
<center><canvas id="main" width="960" height="540" style="display: block; background-color: #f7eff0;"></canvas></center>
+
−
</div>
+
−
<div class="jumbotron"> 
+
−
     <p><b>Developing a cell-free detection system for Chagas disease in the acute phase by detecting biomarkers in the blood</b></p>
+
−
    <p>The aim of our iGEM project is to design a cell-free diagnosis kit to detect the presence of biomarkers specific to the <i>Trypanosoma cruzi</i>, the protozoan parasite responsible for Chagas disease, a neglected tropical disease impacting 6-7 million people primarily in Latin America.</p>
+
−
    <p>Currently, the acute (early) phase of Chagas disease is difficult to diagnose, due to it being asymptomatic and methods using DNA amplification or immunoassays being both expensive and inaccessible to the majority of those affected by Chagas disease. Current treatments using benznidazole and nifurtimox are almost 100% effective if given shortly after the onset of the acute phase. However, lack of diagnosis in the acute phase leads to the onset of the chronic phase, which causes irreversible pathological consequences to the heart, digestive system, and nervous system.</p>
+
−
     <p>The final diagnostic kit would allow Chagas disease to be diagnosed in the acute phase by preventing the clotting of a blood sample, by releasing hirudin (a peptide from leeches) upon cleavage by the protease cruzipain (a cysteine protease specific to <i>T. cruzi</i>). Our system would ultimately be completely cell-free, with the components freeze-dried and competent after rehydration, allowing it to be used in the field without the need for expensive equipment or highly trained professionals - all it will require is a blood sample. We imagine that this system would be adaptable for other parasites and pathogens which have similar difficulties in detection, both in the input for the system and the options for output.</p>
+
−
    <p>We are developing two methods to detect the presence of cruzipain, both being modelled in <i>E. coli</i>. The first is DNA based, and involves the cleavage of a repressor protein to lead to expression of hirudin (modelled using fluorescent proteins and using  TEV instead of cruzipain). The second involves tagging hirudin (again modelled with a fluorophore) to outer membrane vesicles, where the peptide could be cleaved and released from OmpA by cruzipain (modelled with TEV). Our systems would ultimately be completely cell-free, with the components freeze-dried and competent after rehydration.</p>
+
−
    <p>Chagas disease claims over 12000 lives per year. We believe that the death toll could be significantly reduced by a synthetic biology solution, which is cost effective, accessible, and accurate in the swift diagnosis of Chagas disease; a solution that our project aims to provide.</p>
+
−
     <p>If you want to collaborate with us, please send us an email: <u>oxfordigem@bioch.ox.ac.uk</u></p>
+
−
    <p>Follow us on Instagram and Twitter: @OxfordiGEM</p>
+
−
    <p>Please also support us by liking our Facebook page
+
−
    <a href="https://www.facebook.com/oxfordigem/">
+
−
    <img src="https://static.igem.org/mediawiki/2017/e/e4/T--oxford--fbicon.png" alt="Facebook Page" width="20" height="20"></a></p><br></br>
+
−
    <div class="center" > <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/1/10/Oxford_banner.png"> </div>
+
−
    </div>
+
−
</div>
+
−
 
+
−
 
+
−
<script>
+
−
 
+
−
var myGamePiece;
+
−
var cells_size = 250;
+
−
var cells = [cells_size];
+
−
var tCruzi;
+
−
var delay = 0;
+
−
var magnification = 2;
+
−
var rbc_w = 60/2;
+
−
var rbc_h = 53/2;
+
−
var t_cruzi_w = 50;
+
−
var t_cruzi_h = 81; //t cruzi image height
+
−
var magnify_w = 2874;
+
−
var magnify_h = 3475;
+
−
var peek_size = 100;
+
−
var time;
+
−
var checked = false;
+
−
var found = false;
+
−
 
+
−
function startStartScreen(){
+
−
     document.getElementById('main').style.cursor = "block";
+
−
    //document.getElementById('header').style.cursor = "block";
+
−
    //document.getElementById('title').style.cursor = "block";
+
−
    myStartScreen.start();
+
−
    //console.log('startScreen');
+
 
}
 
}
  
−
function startGame() {
+
.btn-circle-lg {
−
     document.getElementById('main').style.cursor = "none";
+
     width: 240px;
−
     //document.getElementById('header').style.cursor = "none";
+
     height: 240px;
−
     //document.getElementById('title').style.cursor = "none";
+
     padding: 10px 16px;
−
     myGamePiece = new createMouse(10, 120);
+
     border-radius: 50%;
−
    myGameArea.start();
+
     font-size: 60px;
−
     initialize();
+
     line-height: 1.33;
−
     //console.log('startGame');
+
 
}
 
}
  
−
function initialize(){
+
.videoWrapper {
−
    for (var i = 0; i < cells_size; i++ ) {
+
position: relative;
−
    var dirr = (Math.random() * 2 * Math.PI);
+
padding-bottom: 56.25%; /* 16:9 */
−
    var posx = (Math.random() * (main.width - 60));
+
padding-top: 25px;
−
    var posy = (Math.random() * (main.height - 53));
+
height: 0;
−
    cells[i] = new createComponent(posx,posy,dirr,"cell");
+
}
−
 
+
.videoWrapper iframe {
−
    }
+
position: absolute;
−
    var dirr = (Math.random() * 2 * Math.PI);
+
top: 0;
−
    var posx = (Math.random() * (main.width - 50));
+
left: 0;
−
    var posy = (Math.random() * (main.height - 81));
+
width: 100%;
−
    tCruzi = new createComponent(posx,posy,dirr,"parasite");
+
height: 100%;
−
    time = new createComponent(30, 30, 0, "time");
+
−
 
+
 
}
 
}
  
 +
.carousel-caption a {
 +
    font-size: 50px;
 +
    font-weight: bolder;
 +
    text-shadow: 1px 3px 5px rgba(0,0,0,0.8);
 +
    color: white;
 +
}
  
−
var myStartScreen = {
+
#button-span {
−
     start : function(){
+
     font-weight: bold;
−
        this.frameNo = 0;
+
    font-size: 20px;
−
        this.interval = setInterval(updateStartScreen, 20);
+
    color: black;
−
        window.addEventListener('mousemove', function(e){
+
    line-height: 0.2;
−
            myStartScreen.x = e.pageX;
+
    text-align:center;
−
            myStartScreen.y = e.pageY-200;
+
     position: relative;
−
        })
+
     left: 0px;
−
        myStartScreen.mouseClick = false
+
     right: 0px;
−
        window.addEventListener('mousedown', function(e){
+
     display: block;
−
            xleft = $("#main").offset().left; //getting the coordinates of the canvas
+
−
            ytop = $("#main").offset().top;
+
−
            xright = $("#main").offset().left + main.width;
+
−
            ybottom = $("#main").offset().top + main.height;
+
−
            //console.log(e.pageX);
+
−
            //console.log(e.pageY);
+
−
            if(e.pageX < xright){
+
−
                if(e.pageX > xleft){
+
−
                    if(e.pageY < ybottom){
+
−
                        if(e.pageY > ytop){
+
−
                            myStartScreen.mouseClick = true;
+
−
                        }
+
−
                    }
+
−
                    //console.log("mouth inside detected");
+
−
                }
+
−
            }
+
−
           
+
−
        })
+
−
        window.addEventListener('resize',function(e){
+
−
            $("#main").outerHeight($(window).height()-$("#main").offset().top- Math.abs($("#main").outerHeight(true) - $("#main").outerHeight()));
+
−
        })
+
−
     },
+
−
     clear : function() {
+
−
        main.getContext("2d").clearRect(0, 0, main.width, main.height);
+
−
     },
+
−
     stop : function(){
+
−
        clearInterval(this.interval);
+
−
    }
+
−
 
+
 
}
 
}
  
−
var myGameArea = {
+
#button-lg-span {
−
     start : function() {
+
     font-weight: bold;
−
        this.frameNo = 0;
+
    font-size: 20px;
−
        this.interval = setInterval(updateGameArea, 20);
+
    color: black;
−
        window.addEventListener('mousemove', function(e){
+
    line-height: 1;
−
            myGameArea.x = e.pageX;
+
    text-align:center;
−
            myGameArea.y = e.pageY-200;
+
    position: relative;
−
        })
+
    left: 0px;
−
        myGameArea.mouseSensor = false;
+
     right: 0px;
−
        window.addEventListener('mousedown', function(e){
+
     display: block;
−
            xleft = $("#main").offset().left; //getting the coordinates of the canvas
+
−
            ytop = $("#main").offset().top;
+
−
            xright = $("#main").offset().left + main.width;
+
−
            ybottom = $("#main").offset().top + main.height;
+
−
            console.log(xleft);
+
−
            console.log(xright);
+
−
            console.log(e.pageX);
+
−
            console.log(ytop);
+
−
            console.log(ybottom);
+
−
            console.log(e.pageY);
+
−
            if(e.pageX < xright){
+
−
                if(e.pageX > xleft){
+
−
                    if(e.pageY < ybottom){
+
−
                        if(e.pageY > ytop){
+
−
                            myGameArea.mouseSensor = true;
+
−
                        }
+
−
                    }
+
−
                }
+
−
            }
+
−
           
+
−
        })
+
−
    },
+
−
     clear : function() {
+
−
        main.getContext("2d").clearRect(0, 0, main.width, main.height);
+
−
     },
+
−
    stop : function() {
+
−
        clearInterval(this.interval);
+
−
 
+
−
    }
+
 
}
 
}
−
 
+
#bodyContent .colourbox {
−
function createMouse(x, y) {
+
  list-style-type: none;
−
    this.x = x;
+
  background-color: #DCE7EF;
−
    this.y = y; 
+
  border-radius: 25px;
−
 
+
}
−
    this.update = function(){
+
#bodyContent ul{
−
        main.style.display = "block";
+
   list-style-type: none;
−
        ctx = main.getContext("2d");
+
}
−
        var img = document.getElementById("magnify-glass");
+
#circles_home {
−
        ctx.drawImage(img, this.x-magnify_w/22-13, this.y-magnify_w/22+13, magnify_w/11, magnify_h/11);
+
         margin: 50px 50px 50px 80px;
−
        ctx.save();
+
}
−
        ctx.beginPath();
+
#texthome{
−
        ctx.arc(this.x, this.y, peek_size, 0, 2 * Math.PI);
+
  width: 80%;
−
        ctx.fillStyle = 'rgb(255, 237, 237)';
+
−
        ctx.fill();
+
−
        //ctx.lineWidth = 3;
+
−
        ctx.strokeStyle = '#003300';
+
−
        //ctx.stroke();
+
−
        ctx.clip();
+
−
        ctx.restore()
+
−
    }
+
−
 
+
−
    this.updateFound = function(){
+
−
        main.style.display = "block";
+
−
        ctx = main.getContext("2d");
+
−
        var img = document.getElementById("magnify-glass");
+
−
        ctx.drawImage(img, this.x-magnify_w/22-13, this.y-magnify_w/22+13, magnify_w/11, magnify_h/11);
+
−
        ctx.save();
+
−
        ctx.beginPath();
+
−
        ctx.arc(this.x, this.y, peek_size, 0, 2 * Math.PI);
+
−
        ctx.fillStyle = "rgba(0,33,71,.5)";
+
−
        ctx.fill();
+
−
        //ctx.lineWidth = 3;
+
−
        ctx.strokeStyle = '#003300';
+
−
        ctx.font = "20px Calibri";
+
−
        ctx.fillStyle = "#FFFFFF";
+
−
        this.text1 = "              Yay!"
+
−
        this.text2 = "      You've found"
+
−
        this.text3 = " it in " + Math.round(myGameArea.frameNo*20/1000) + " seconds. In"
+
−
        this.text4 = " practice, it takes 5-10"
+
−
        this.text5 = "   minutes for a sample"
+
−
        this.text6 = "  of blood under the"
+
−
        this.text7 = "        microscope."
+
−
        ctx.fillText(this.text1, this.x-80, this.y-80);
+
−
        ctx.fillText(this.text2, this.x-80, this.y-60);
+
−
        ctx.fillText(this.text3, this.x-80, this.y-40);
+
−
        ctx.fillText(this.text4, this.x-90, this.y-20);
+
−
        ctx.fillText(this.text5, this.x-100, this.y);
+
−
        ctx.fillText(this.text6, this.x-90, this.y+20);
+
−
        ctx.fillText(this.text7, this.x-80, this.y+40);
+
−
        ctx.font = "15px Calibri";
+
−
        ctx.fillStyle = "#FFFFFF";
+
−
        this.text9 = "click for"
+
−
        ctx.fillText(this.text9, this.x-20, this.y+60);
+
−
 
+
−
 
+
−
        ctx.font = "35px Calibri";
+
−
        ctx.fillStyle = "#1569d6";
+
−
        this.text8 = "RESTART"
+
−
        ctx.fillText(this.text8, this.x-58, this.y+90);
+
−
 
+
−
        //ctx.stroke();
+
−
        //ctx.clip();
+
−
        ctx.restore();
+
−
        if (myGameArea.mouseSensor) {
+
−
         checked = false;
+
−
        found=false;
+
−
        myGameArea.stop();
+
−
        startStartScreen();
+
−
        //console.log("mouse detected after found");
+
−
    }
+
−
 
+
−
    }
+
−
 
+
−
    this.found = function(){
+
−
        var distance_x = Math.abs(this.x - tCruzi.x - t_cruzi_w/8);
+
−
        var distance_y = Math.abs(this.y - tCruzi.y - t_cruzi_h/8);
+
−
        if ((distance_x*distance_x + distance_y*distance_y) < 100){
+
−
            return true;
+
−
        }
+
−
        else {return false;}
+
−
    }
+
 
}
 
}
  
−
function createComponent(x, y, dir, type){
+
</style>
−
    this.type = type;
+
</head>
−
    this.x = x;
+
<body onload="startStartScreen()">
−
    this.y = y;
+
−
    this.dir = dir;
+
−
    this.is_peeking = false;
+
−
    this.dx = 0;
+
−
    this.dy = 0;
+
−
    this.distance = 0;
+
−
    this.peek_calc = function(on_canvas){
+
−
        if (on_canvas)
+
−
        {
+
−
            this.dx = myGameArea.x - this.x;
+
−
            this.dy = myGameArea.y - this.y;
+
−
            this.distance = Math.sqrt(this.dx*this.dx + this.dy*this.dy);
+
−
            this.is_peeking = (this.distance < peek_size * 1.5)? true:false;
+
−
        }
+
−
        else
+
−
        {
+
−
            this.is_peeking = false;
+
−
        }
+
−
    }
+
−
    this.draw_main = function(){
+
−
            ctx = main.getContext("2d");
+
−
            if(this.type == "cell"){
+
−
               
+
−
                var img = document.getElementById("red-blood-cell");
+
−
                ctx.drawImage(img, this.x, this.y, rbc_w, rbc_h);
+
−
        }
+
−
            if(this.type == "parasite"){
+
−
               
+
−
                var img = document.getElementById("t-cruzi");
+
−
                ctx.drawImage(img, this.x, this.y, t_cruzi_w/4, t_cruzi_h/4);
+
−
            }
+
−
            //if(this.type)
+
−
    }
+
  
−
    this.draw_peek = function(){
+
<div class="container"> 
−
        if (this.is_peeking)
+
<div style="margin-top: 60px"></div>
−
        {
+
<div class="row">
−
            ctx = main.getContext("2d");
+
    <h1 style="line-height:60px">OXFORD iGEM 2017<br/><small><i>See cruzi </i></small><br/></h1>
−
            ctx.save();
+
</div>
−
            ctx.beginPath();
+
−
            ctx.arc(myGameArea.x, myGameArea.y, peek_size, 0, 2 * Math.PI);
+
−
            ctx.clip();
+
−
            if(this.type == "cell"){
+
−
           
+
−
                var img = document.getElementById("red-blood-cell");
+
−
                ctx.drawImage(img, (this.x - this.dx * magnification), (this.y - this.dy * magnification), rbc_w * magnification * 1.5, rbc_h * magnification * 1.5);
+
−
        }
+
−
            if(this.type == "parasite"){
+
−
             
+
−
                var img = document.getElementById("t-cruzi");
+
−
                ctx.drawImage(img, (this.x - this.dx * magnification), (this.y - this.dy * magnification), t_cruzi_w/4 * magnification * 1.5, t_cruzi_h/4 * magnification * 1.5);
+
−
            }
+
  
−
            ctx.restore();
+
  <div class="row">
−
        }
+
      <div id="myCarousel" class="carousel slide" data-ride="carousel">
−
     }
+
  <!-- Indicators -->
 +
  <ol class="carousel-indicators">
 +
     <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
 +
    <li data-target="#myCarousel" data-slide-to="1"></li>
 +
    <li data-target="#myCarousel" data-slide-to="2"></li>
 +
    <li data-target="#myCarousel" data-slide-to="3"></li>
 +
    <li data-target="#myCarousel" data-slide-to="4"></li>
 +
  </ol>
  
−
    this.move = function(){
+
  <!-- Wrapper for slides -->
 +
  <div class="carousel-inner">
  
−
        var newXDirection = 0.1*Math.cos(this.dir);
+
    <div class="item active">
−
        var newYDirection = 0.1*Math.sin(this.dir);
+
    <img src="https://static.igem.org/mediawiki/2017/9/92/T--Oxford--NewHome--awardresize.jpg" alt="Award">
−
        this.x += newXDirection;
+
<div class="carousel-caption">
−
        this.y += newYDirection;
+
    <a href="https://2017.igem.org/Team:Oxford/Team">
 +
    <h2> MEET THE TEAM! </h2>
 +
    </a>
 +
    </div>
 +
    </div>
  
−
        var bottomline = main.height - rbc_h;
+
    <div class="item">
−
        var rightline = main.width - rbc_w;
+
<img src="https://static.igem.org/mediawiki/2017/1/18/T--Oxford--NewHome--Posterresize.jpg" alt="Poster">
−
        if(this.y > bottomline || this.x > rightline || this.y < 0 || this.x < 0){
+
    <div class="carousel-caption">
−
         
+
    <a href="https://2017.igem.org/Team:Oxford/Team">
−
            this.x -= newXDirection*2;
+
    <h2> MEET THE TEAM! </h2>
−
            this.y -= newYDirection*2;
+
    </a>
 +
    </div>
 +
    </div>
  
−
        }
+
    <div class="item">
−
       
+
      <img src="https://static.igem.org/mediawiki/2017/8/81/T--Oxford--NewHome--Meetingresize.jpg" alt="Meeting">
−
     }
+
<div class="carousel-caption">
 +
    <a href="https://2017.igem.org/Team:Oxford/Team">
 +
    <h2> MEET THE TEAM! </h2>
 +
    </a>
 +
    </div>
 +
     </div>
  
−
    this.updateTime = function(){
+
<div class="item">
−
        ctx.font = "30px Arial";
+
      <img src="https://static.igem.org/mediawiki/2017/0/0a/T--Oxford--NewHome--Jokesresize.jpg" alt="Jokes">
−
        ctx.fillStyle = "#000000";
+
<div class="carousel-caption">
−
        ctx.fillText(this.text, this.x, this.y);
+
    <a href="https://2017.igem.org/Team:Oxford/Team">
−
     }
+
    <h2> MEET THE TEAM! </h2>
 +
    </a>
 +
    </div>
 +
     </div>
  
−
}
+
    <div class="item">
 +
      <img src="https://static.igem.org/mediawiki/2017/2/2c/T--Oxford--NewHome--Ladiesresize.jpg" alt="Ladies">
 +
<div class="carousel-caption">
 +
    <a href="https://2017.igem.org/Team:Oxford/Team">
 +
    <h2> MEET THE TEAM! </h2>
 +
    </a>
 +
    </div>
 +
    </div>
 +
  </div>
  
−
function centerText(ctx, text, y) {
+
  <!-- Left and right controls -->
−
     var measurement = ctx.measureText(text);
+
  <a class="left carousel-control" href="#myCarousel" data-slide="prev">
−
     var x = (ctx.canvas.width - measurement.width) / 2;
+
     <span class="glyphicon glyphicon-chevron-left"></span>
−
     ctx.fillText(text, x-50, y);
+
     <span class="sr-only">Previous</span>
−
}
+
  </a>
 +
  <a class="right carousel-control" href="#myCarousel" data-slide="next">
 +
     <span class="glyphicon glyphicon-chevron-right"></span>
 +
    <span class="sr-only">Next</span>
 +
  </a>
 +
</div>
  
−
var title = 'Finding      T. Cruzi';
+
<div class="row">
−
var hue = 0;
+
      <div class="col-sm-6">
−
var direction = 1;
+
        <a href="https://2017.igem.org/Team:Oxford/Awards">
−
var ctx = main.getContext('2d');
+
        <img class="img-responsive img-center" style="width:100%" src="https://static.igem.org/mediawiki/2017/f/f4/T--Oxford--NewHome--Prizesbox.png">
−
var wasButtonDown = false;
+
        </a>
 +
      </div>
 +
      <div class="col-sm-6">
 +
        <a class="twitter-timeline" data-width="100%" data-height="500" href="https://twitter.com/OxfordiGEM?ref_src=twsrc%5Etfw">Tweets by OxfordiGEM</a> <script async src="https://platform.twitter.com/widgets.js" charset="utf-8"></script>
 +
      </div>
 +
  </div>
  
−
function updateStartScreen(){
+
<div class="row">
−
    hue += 1 * direction;
+
<div class="col-sm-12">
−
    if (hue > 255) direction = -1;
+
<div class="embed-responsive embed-responsive-16by9">
−
    if (hue < 78) direction = 1;
+
  <iframe class="embed-responsive-item" src="https://www.youtube.com/embed/4heo3c2qvWo" allowfullscreen></iframe>
 +
</div>
 +
</div>
 +
</div>
  
−
    var y = ctx.canvas.height / 2;
+
</div>
−
    var color = 'rgb(7,38,' + hue + ')';
+
  
−
    myStartScreen.clear();
+
<div class="container"> 
 +
<div style="margin-top: 60px"></div>
 +
<div class="row">
 +
<h2> Our Project </h2><br/>
 +
</div>
 +
<div class="row" align="centre">
  
−
    var img = document.getElementById("start_screen_img");
+
<a href="https://2017.igem.org/Team:Oxford/Game"> <img class="img-responsive img-center" src="https://static.igem.org/mediawiki/2017/thumb/a/a6/T--oxford--play2.png/320px-T--oxford--play2.png"> </a>
−
    //console.log(main.width);
+
−
    //console.log(main.height);
+
−
    var screen_width_height_ratio = main.width/main.height;
+
−
    console.log(screen_width_height_ratio);
+
−
    var ssi_width_height_ratio = start_screen_img.width/start_screen_img.height;//ssi = start screen img
+
−
    //console.log(ssi_width_height_ratio);
+
−
    if(screen_width_height_ratio>ssi_width_height_ratio){
+
−
        var start_screen_x = (main.width-main.height*ssi_width_height_ratio)/2;
+
−
        ctx.drawImage(img, start_screen_x, 0, main.height*ssi_width_height_ratio, main.height);
+
−
    }
+
−
    if(screen_width_height_ratio<ssi_width_height_ratio){
+
−
        var start_screen_y = (main.height-main.width/ssi_width_height_ratio)/2;
+
−
        ctx.drawImage(img, start_screen_x, 0, main.width, main.width/ssi_width_height_ratio);
+
−
    }
+
  
−
    ctx.fillStyle = 'white';
+
<p><br/>The aim of our project was to develop a cell-free detection system for congenital Chagas disease in the acute phase by detecting the cruzipain in the blood.  
−
    ctx.font = '48px monospace';
+
Congenital Chagas disease is a growing global concern and at present, the acute phase of Chagas disease is difficult to diagnose as it is asymptomatic and current diagnostics are both expensive and inaccessible to the majority of those affected. Our final diagnostic kit would allow Chagas disease to be detected by preventing the clotting of a blood sample. </p>
−
    centerText(ctx, title, main.height*0.4);
+
<p> We imagine that this system would be adaptable for other parasites and pathogens which have similar difficulties in detection, both in the input for the system and the options for output.</p>
−
 
+
   </div>
−
    ctx.fillStyle = color;
+
</div>
−
    ctx.font = '40px Helvetica';
+
</div>
−
    centerText(ctx, 'click to begin', main.height*0.9);
+
−
 
+
−
 
+
−
    if (myStartScreen.mouseClick) {
+
−
      // do something here to transition to the actual game
+
−
      myStartScreen.stop();
+
−
      startGame();
+
−
      //console.log('startgamefromclick');
+
−
     
+
−
    }
+
−
}
+
−
 
+
−
 
+
−
function updateGameArea() {
+
−
    var on_screen = false;
+
−
    if (myGameArea.x){
+
−
        if(myGameArea.y){
+
−
        myGamePiece.x = myGameArea.x;
+
−
        myGamePiece.y = myGameArea.y;    
+
−
        on_screen = true;
+
−
        }   
+
−
    }
+
  
 +
<div class="container"> 
 +
<div style="margin-top: 60px"></div>
 +
<div class="row">
 +
    <div class="col-sm-3">
 +
    <form action="https://2017.igem.org/Team:Oxford/Description">
 +
  <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(72,145,220)"><span id="button-lg-span">Project<br>Description</span></button>
 +
    </form>
 +
    </div>
 
      
 
      
−
     myGameArea.clear();
+
     <div class="col-sm-3">
−
     if(checked == false){
+
    <form action="https://2017.igem.org/Team:Oxford/Overview_Wet_Lab">
−
        found = myGamePiece.found();
+
     <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(234,139,68)"><span id="button-lg-span">Wet Lab<br>Overview</span></button>
−
        if(found) {
+
     </form>
−
            checked = true;
+
     </div>
−
            //console.log("checked = true")
+
−
            //myGameArea.stop();
+
−
        }
+
−
     }
+
−
 
+
−
     if(!found){myGameArea.frameNo += 1;} //stop the timing when t cruzi is found
+
  
 +
    <div class="col-sm-3">
 +
    <form action="https://2017.igem.org/Team:Oxford/Model">
 +
    <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(185, 207, 150)"><span id="button-lg-span">Dry Lab<br>Overview</span></button>
 +
    </form>
 +
    </div>
 
      
 
      
−
 
+
    <div class="col-sm-3">
−
     myGamePiece.update();
+
     <form action="https://2017.igem.org/Team:Oxford/Applied_Design">
 +
    <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(95, 155, 175)"><span id="button-lg-span">Applied<br>Design</span></button>
 +
    </form>
 +
    </div>
 
      
 
      
−
    delay += 1;
+
</div>
−
    if (delay >= 2) {
+
</div>
−
        for (var i = 0; i < cells.length; i++) {
+
−
            cells[i].dir += (Math.random() * Math.PI/5) - (Math.PI/10);
+
−
            cells[i].move();
+
−
            cells[i].peek_calc(on_screen);
+
−
        }
+
−
        tCruzi.dir += (Math.random() * Math.PI/5) - (Math.PI/10);
+
−
        tCruzi.move();
+
−
        tCruzi.peek_calc(on_screen);
+
−
    }
+
−
   
+
−
    for (var i = 0; i < cells.length; i++ ) {
+
−
        //console.log("cell" + i + "is updated");
+
−
        cells[i].draw_main();
+
−
       
+
−
    }
+
−
    //tCruzi.draw_main();
+
−
   
+
−
 
+
−
 
+
−
    myGamePiece.update();
+
−
    //console.log(tCruzi.type);
+
−
    for (var i = 0; i < cells.length; i++ ) {
+
−
        //console.log("cell" + i + "is updated");
+
−
        cells[i].draw_peek();
+
−
       
+
−
    }
+
−
    tCruzi.draw_peek();
+
−
 
+
−
    time.text="time elapsed: " + Math.round(myGameArea.frameNo*20/1000) + " seconds";
+
−
    time.updateTime();
+
−
 
+
−
 
+
−
if (found)
+
−
    {myGamePiece.updateFound();
+
−
        //console.log("third update");
+
−
    }
+
−
}
+
−
 
+
−
</script>
+
  
 
</body>
 
</body>
  
 
</html>
 
</html>
 +
{{Oxford/footer}}

Latest revision as of 23:37, 15 December 2017

OXFORD iGEM 2017
See cruzi

Our Project



The aim of our project was to develop a cell-free detection system for congenital Chagas disease in the acute phase by detecting the cruzipain in the blood. Congenital Chagas disease is a growing global concern and at present, the acute phase of Chagas disease is difficult to diagnose as it is asymptomatic and current diagnostics are both expensive and inaccessible to the majority of those affected. Our final diagnostic kit would allow Chagas disease to be detected by preventing the clotting of a blood sample.

We imagine that this system would be adaptable for other parasites and pathogens which have similar difficulties in detection, both in the input for the system and the options for output.