Difference between revisions of "Team:Dalhousie/anothertest"

 
(82 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
<html lang="en">
 
<html lang="en">
  
 
<head>
 
<head>
−
 
+
<!--glyphicons-->
−
 
+
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
  
 
<!--just added all this shit-->
 
<!--just added all this shit-->
Line 35: Line 34:
 
});
 
});
 
</script>
 
</script>
 +
 +
 
  <style>
 
  <style>
 
body, html, .main {
 
body, html, .main {
Line 45: Line 46:
  
  
 +
<!--fullpage settings-->
 +
#home_logo, #sideMenu { display:none; }
 +
#sideMenu, #top_title, .patrollink  {display:none;}
 +
#content { width:100%; padding:0px;  margin-top:-42px; margin-left:0px; margin-bottom:50px;}</style>
  
  
−
 
−
 
−
#home_logo, #sideMenu { display:none; }
 
−
#sideMenu, #top_title, .patrollink  {display:none;}
 
−
#content { width:100%; padding:0px;  margin-top:-42px; margin-left:0px;}</style>
 
−
<link rel="stylesheet" href="https://2017.igem.org/Team:Dalhousie/libraries/css/fonts?action=raw&ctype=text/css" />
 
  
 
     <meta charset="utf-8">
 
     <meta charset="utf-8">
 
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
 
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
 
     <meta name="viewport" content="width=device-width, initial-scale=1">
 
     <meta name="viewport" content="width=device-width, initial-scale=1">
−
    <meta name="description" content="">
 
−
    <meta name="author" content="">
 
  
 
     <title>Dalhousie iGem</title>
 
     <title>Dalhousie iGem</title>
  
 
     <!-- Bootstrap Core CSS -->
 
     <!-- Bootstrap Core CSS -->
−
    <link href="https://2017.igem.org/wiki/index.php?title=Team:Dalhousie/bootstrapmincss&action=raw&ctype=text/css" rel="stylesheet">
+
  <link href="https://2017.igem.org/wiki/index.php?title=Team:Dalhousie/bootstrapmincss&action=raw&ctype=text/css" rel="stylesheet">
  
−
    <!-- Custom Fonts -->
+
<!-- Custom Fonts -->
−
    <link href="https://2017.igem.org/wiki/index.php?title=Team:Dalhousie/fontawesome&action=raw&ctype=text/css" rel="stylesheet" type="text/css">
+
<link href="https://2017.igem.org/wiki/index.php?title=Team:Dalhousie/fontawesome?action=raw&ctype=text/css" rel="stylesheet" type="text/css">
 
     <link href="https://fonts.googleapis.com/css?family=Lora:400,700,400italic,700italic" rel="stylesheet" type="text/css">
 
     <link href="https://fonts.googleapis.com/css?family=Lora:400,700,400italic,700italic" rel="stylesheet" type="text/css">
 
     <link href="https://fonts.googleapis.com/css?family=Montserrat:400,700" rel="stylesheet" type="text/css">
 
     <link href="https://fonts.googleapis.com/css?family=Montserrat:400,700" rel="stylesheet" type="text/css">
 +
    <link href="https://2017.igem.org/Team:Dalhousie/libraries/css/fonts?action=raw&ctype=text/css" rel="stylesheet" type="text/css">
  
−
    <!-- Theme CSS -->
 
−
    <link href="https://2017.igem.org/wiki/index.php?title=Team:Dalhousie/grayscale&action=raw&ctype=text/css" rel="stylesheet">
 
  
 +
<!-- Theme CSS -->
 +
<link href="https://2017.igem.org/wiki/index.php?title=Team:Dalhousie/grayscale&action=raw&ctype=text/css" rel="stylesheet">
 +
 +
   
 +
<!------------------------no idea what this is-------------------------->
 
     <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries -->
 
     <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries -->
 
     <!-- WARNING: Respond.js doesn't work if you view the page via file:// -->
 
     <!-- WARNING: Respond.js doesn't work if you view the page via file:// -->
Line 80: Line 81:
 
     <![endif]-->
 
     <![endif]-->
  
−
</head>
+
<!----------------------------------------------navbarshit---------------------------------------------->
  
−
<body id="page-top" data-spy="scroll" data-target=".navbar-fixed-top">
 
  
−
    <!-- Navigation -->
+
  <meta charset="utf-8">
−
    <nav class="navbar navbar-custom navbar-fixed-top" role="navigation">
+
  <meta name="viewport" content="width=device-width, initial-scale=1">
−
        <div class="container">
+
  <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
−
            <div class="navbar-header">
+
  <script src="https://ajax.googleapis.com/ajax/libs/jquery/3.2.1/jquery.min.js"></script>
−
                <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-main-collapse">
+
  <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
−
                    Menu <i class="fa fa-bars"></i>
+
</head>
−
                </button>
+
−
               
+
−
            </div>
+
  
−
            <!-- Collect the nav links, forms, and other content for toggling -->
 
−
            <div class="collapse navbar-collapse navbar-right navbar-main-collapse">
 
−
                <ul class="nav navbar-nav">
 
−
                    <!-- Hidden li included to remove active class from about link when scrolled up past about section -->
 
−
                    <li class="hidden">
 
−
                        <a href="#page-top"></a>
 
−
                    </li>
 
  
  
  
  
 +
    <!------------------------------------------------------------------------------- Navbar -------------------------------------------------------------------------->
 +
<nav class="navbar navbar-inverse navbar-fixed-bottom">
 +
  <div class="container-fluid">
 +
   
 +
    <ul class="nav navbar-nav navbar-center">
 +
      <li class="active"><a href="https://2017.igem.org/Team:Dalhousie/anothertest">Home</a></li>
 +
      <li class="dropdown">
 +
        <a class="dropdown-toggle" data-toggle="dropdown" href="#">The Project
 +
        <span class="caret"></span></a>
 +
        <ul class="dropdown-menu">
 +
          <li><a href="#">Design</a></li>
 +
          <li><a href="#">Experiments</a></li>
 +
          <li><a href="#">Notebook</a></li>
 +
          <li><a href="#">Results</a></li>
 +
          <li><a href="#">Safety</a></li>
 +
        </ul>
 +
      </li>
  
−
                    <li>
+
      <li><a href="#">Our Team</a></li>
−
                         <a class="page-scroll" href="#about">About Us</a>
+
      <li><a href="#">Attributions</a></li>
 +
<li><a href="#">Outreach</a></li>
 +
<li><a href="https://2017.igem.org/Team:Dalhousie/Human_Practices">Human practices</a></li>
 +
    </ul>
 +
<ul class="nav navbar-nav navbar-right">
 +
    <div class="navbar-header">
 +
      <a class="navbar-brand" >Contact us today </a>
 +
    </div>
 +
<li>
 +
                         <a href="mailto:igemdalhousie@gmail.com" ><i class="fa fa-envelope-o fa-fw"></i> <span class="network-name"></span></a>
 +
                    </li>
 +
<li>
 +
                        <a href="https://twitter.com/dalhousie_igem?lang=en" ><i class="fa fa-twitter fa-fw"></i> <span class="network-name"></span></a>
 
                     </li>
 
                     </li>
−
                   
 
 
                     <li>
 
                     <li>
−
                         <a class="page-scroll" href="#contact">Contact</a>
+
                         <a href="https://www.facebook.com/Dalhousie.iGEM/" ><i class="fa fa-facebook fa-fw"></i> <span class="network-name"></span></a>
 
                     </li>
 
                     </li>
−
                </ul>
+
    </ul>
−
            </div>
+
  </div>
−
            <!-- /.navbar-collapse -->
+
</nav>
−
        </div>
+
   
−
        <!-- /.container -->
+
             
−
    </nav>
+
 
 +
 
 +
 
 +
       
  
 
     <!-- Intro Header -->
 
     <!-- Intro Header -->
Line 126: Line 147:
 
                 <div class="row">
 
                 <div class="row">
 
                     <div class="col-md-8 col-md-offset-2">
 
                     <div class="col-md-8 col-md-offset-2">
−
                         <h1 class="brand-heading">Dalhousie iGem</h1>
+
                         <h1 class="brand-heading">Dalhousie iGEM</h1>
 
                        
 
                        
−
                         <a href="#about" class="btn btn-circle page-scroll">
+
 
−
                             <i class="fa fa-angle-double-down animated"></i>
+
<!---------------------button----------------------------------------------------->
 +
 
 +
 
 +
 
 +
                         <a href="#about" class="btn btn-link btn-lg  page-scroll">
 +
                             <span class="glyphicon glyphicon-arrow-down" aria-hidden="true"></span>
 
                         </a>
 
                         </a>
 +
 +
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
Line 136: Line 164:
 
         </div>
 
         </div>
 
     </header>
 
     </header>
−
 
−
 
−
 
−
 
−
 
−
 
−
 
  
 
     <!-- About Section -->
 
     <!-- About Section -->
Line 148: Line 169:
 
         <div class="row">
 
         <div class="row">
 
             <div class="col-lg-8 col-lg-offset-2">
 
             <div class="col-lg-8 col-lg-offset-2">
−
                <h2>About us</h2>
+
             
−
                <p style="text-align:center">>this is where we write stuff. </p>
+
             
 +
<h4>Background</h4>
 +
<p style="text-align:center">  
 +
Canada’s forests represent not only an established source of economic revenue, but also a potential source for biofuel substrate. Ligno-cellulosic by-products from pulp and paper processing are removed using chemicals, heat, and water. Microbial cellulose-degrading enzymes are high-value targets for industrial applications. Focused on innovative applications of synthetic biology, the undergraduate Dalhousie iGEM team has undertaken a multi-year project to harness the degradative capacity of microorganisms to convert cellulose into ethanol for biofuel applications. Advances in DNA sequencing technology and bioinformatics have revolutionized our ability to identify useful genes in complex biological samples. We hypothesize that the porcupine microbiome, which includes microorganisms capable of digesting bark and tree resin, will be a rich source of these useful genes. If our hypothesis is correct, mining the porcupine microbiome has the advantage of finding a suite of enzymes that have evolved to work in concert to efficiently degrade cellulose. </p>
 +
 +
<h4>The Project</h4>
 +
<p style="text-align:center">
 +
Using a combination of metagenomic library construction and bioinformatic analysis we aim to find known cellulolytic enzymes as well as discover novel enzymes.
 +
Screening of our metagenomic library will allow us to look for a diverse selection of enzymes, including cellulolytic and lignolytic ones by plating on cellulose-only media. The clones that grow can be sequenced for confirmation and cloned into biobricks.
 +
Our new bioinformatic pipeline now allows us to look for conserved domains rather than known enzymes. This is extremely powerful as it may allow us to discover new enzymes that share catalytic domains with cellulases but are otherwise totally novel.
 +
The goal of this project is to construct a bioreactor where cellulose would be the input, D-glucose the output and E.coli expressing a suite of cellulolytic enzymes would be the workhorse, converting one to the other. Future goals would be to combine this in co-culture with yeast for the last step in bio-ethanol production.</p>
 +
 
 +
 
 
                  
 
                  
 
                  
 
                  
Line 158: Line 191:
 
    
 
    
  
−
   <!-- Contact Section -->
+
    
−
    <section id="contact" class="content-section text-center">
+
−
        <div class="download-section">
+
−
            <div class="container">
+
−
                <div class="col-lg-8 col-lg-offset-2">
+
−
                    <h2>Contact us</h2>
+
−
                    <p style="text-align:center">Feel free to contact us to collab, or to just say hello!</p>
+
−
               
+
−
                <ul class="list-inline banner-social-buttons">
+
−
                   
+
−
<li>
+
−
                        <a href="mailto:igemdalhousie@gmail.com" class="btn btn-default btn-lg"><i class="fa fa-google-plus fa-fw"></i> <span class="network-name">Email+</span></a>
+
−
                    </li>
+
−
<li>
+
−
                        <a href="https://twitter.com/dalhousie_igem?lang=en" class="btn btn-default btn-lg"><i class="fa fa-twitter fa-fw"></i> <span class="network-name">Twitter</span></a>
+
−
                    </li>
+
−
                    <li>
+
−
                        <a href="https://www.facebook.com/Dalhousie.iGEM/" class="btn btn-default btn-lg"><i class="fa fa-github fa-fw"></i> <span class="network-name">Facebook</span></a>
+
−
                    </li>
+
−
                   
+
−
                    </li>
+
−
                </ul>
+
−
                </div>
+
−
            </div>
+
−
        </div>
+
−
    </section>
+
−
 
+
  
  
Line 197: Line 204:
 
     <script src="https://cdnjs.cloudflare.com/ajax/libs/jquery-easing/1.3/jquery.easing.min.js"></script>
 
     <script src="https://cdnjs.cloudflare.com/ajax/libs/jquery-easing/1.3/jquery.easing.min.js"></script>
  
−
     <!-- Google Maps API Key - Use your own API key to enable the map feature. More information on the Google Maps API can be found at https://developers.google.com/maps/ -->
+
      
−
    <script type="text/javascript" src="https://maps.googleapis.com/maps/api/js?key=AIzaSyCRngKslUGJTlibkQ3FkfTxj3Xss1UlZDA&sensor=false"></script>
+
  
 
     <!-- Theme JavaScript -->
 
     <!-- Theme JavaScript -->

Latest revision as of 20:16, 10 August 2017

Dalhousie iGem

Dalhousie iGEM

Background

Canada’s forests represent not only an established source of economic revenue, but also a potential source for biofuel substrate. Ligno-cellulosic by-products from pulp and paper processing are removed using chemicals, heat, and water. Microbial cellulose-degrading enzymes are high-value targets for industrial applications. Focused on innovative applications of synthetic biology, the undergraduate Dalhousie iGEM team has undertaken a multi-year project to harness the degradative capacity of microorganisms to convert cellulose into ethanol for biofuel applications. Advances in DNA sequencing technology and bioinformatics have revolutionized our ability to identify useful genes in complex biological samples. We hypothesize that the porcupine microbiome, which includes microorganisms capable of digesting bark and tree resin, will be a rich source of these useful genes. If our hypothesis is correct, mining the porcupine microbiome has the advantage of finding a suite of enzymes that have evolved to work in concert to efficiently degrade cellulose.

The Project

Using a combination of metagenomic library construction and bioinformatic analysis we aim to find known cellulolytic enzymes as well as discover novel enzymes. Screening of our metagenomic library will allow us to look for a diverse selection of enzymes, including cellulolytic and lignolytic ones by plating on cellulose-only media. The clones that grow can be sequenced for confirmation and cloned into biobricks. Our new bioinformatic pipeline now allows us to look for conserved domains rather than known enzymes. This is extremely powerful as it may allow us to discover new enzymes that share catalytic domains with cellulases but are otherwise totally novel. The goal of this project is to construct a bioreactor where cellulose would be the input, D-glucose the output and E.coli expressing a suite of cellulolytic enzymes would be the workhorse, converting one to the other. Future goals would be to combine this in co-culture with yeast for the last step in bio-ethanol production.