Difference between revisions of "Team:Newcastle/Results"

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           <p>In order to implement the Formaldehyde biosensor variant to the Sensynova platform, a design was created by replacing the IPTG sensing system in the original detector module with the construct detailed above, creating part <a href="http://parts.igem.org/Part:BBa_K2205030">BBa_K2205030 </a>.   
 
           <p>In order to implement the Formaldehyde biosensor variant to the Sensynova platform, a design was created by replacing the IPTG sensing system in the original detector module with the construct detailed above, creating part <a href="http://parts.igem.org/Part:BBa_K2205030">BBa_K2205030 </a>.   
 
           </br></br>
 
           </br></br>
We chose to redesign the Formaldehyde biosensor detailed above to mirror the design used when producing the Psicose detector variant. The system detailed in the image below is made up of the constitutive promoter present within the platform triggering transcription of the FrmR repressing the PfrmR and subsequently the connector 1 of the Sensynova platform. We have also replaced the colour output present in the TMU-Tokyo design, we have added our part K2205008, which produces our first connector in order to trigger a response from following modules of the Sensynova platform in the presence of Formaldehyde.</p>
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We chose to redesign the Formaldehyde biosensor detailed above to mirror the design used when producing the Psicose detector variant. The system detailed in the image below is made up of the constitutive promoter present within the platform triggering transcription of the FrmR repressing the PfrmR and subsequently the connector 1 of the Sensynova platform. We have also replaced the colour output present in the TMU-Tokyo design, we have added our part <a href="http://parts.igem.org/Part:BBa_K2205008">BBa_K2205008</a>, which produces our first connector in order to trigger a response from following modules of the Sensynova platform in the presence of Formaldehyde.</p>
  
 
           <img src=" https://static.igem.org/mediawiki/2017/9/9e/T--Newcastle--Lais--FO--Design2.jpg " class="img-fluid border border-dark rounded" style="margin: 2%">
 
           <img src=" https://static.igem.org/mediawiki/2017/9/9e/T--Newcastle--Lais--FO--Design2.jpg " class="img-fluid border border-dark rounded" style="margin: 2%">
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           <h2 style="font-family: Rubik; text-align: left; margin-top: 1%"> References </h2>
 
           <h2 style="font-family: Rubik; text-align: left; margin-top: 1%"> References </h2>
           <p>iGEM Community. (2012). Team TMU-Tokyo 2012. [online] Available at: https://2012.igem.org/Team:TMU-Tokyo [Accessed 1 Nov. 2017].
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           <p>iGEM Community (2012). Team TMU-Tokyo 2012. [online] Available at: https://2012.igem.org/Team:TMU-Tokyo [Accessed 1 Nov. 2017].
 
           </p>
 
           </p>
  

Revision as of 16:59, 1 November 2017

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Our Experimental Results


Below is a diagram of our Sensynova Framework. Clicking on each part of the framework (e.g. detector modules) links to the relevant results.

Alternatively, at the bottom of this page are tabs which will show you results for every part of the project



Framework

Framework Chassis

Biochemical Adaptor

Target

Detector Modules

Multicellular Framework Testing

C12 HSL: Connector 1

Processor Modules

Framework in Cell Free Protein Synthesis Systems

C4 HSL: Connector 2

Reporter Modules



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