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<li>Improve previous part or project: | <li>Improve previous part or project: | ||
We improved a T7 promtore with RBS by adding a sequence in it. | We improved a T7 promtore with RBS by adding a sequence in it. | ||
− | <a href="http://parts.igem.org/Part:BBa_K2382003" target="_blank"><img src="https://static.igem.org/mediawiki/2017/6/66/T--CSMU_NCHU_Taiwan--see_more.png" alt=""style="cursor: pointer ;width:120px"></a> | + | <a href="http://parts.igem.org/Part:BBa_K2382003" target="_blank"><img src="https://static.igem.org/mediawiki/2017/6/66/T--CSMU_NCHU_Taiwan--see_more.png" alt=""style="cursor: pointer ;width:120px"></a> <br> |
We also modified the widely used RFP by removing the barcode after its sequence, and did codon optimization for E.coli as a better part for fusion protein. | We also modified the widely used RFP by removing the barcode after its sequence, and did codon optimization for E.coli as a better part for fusion protein. | ||
<a href="http://parts.igem.org/Part:BBa_K2382013" target="_blank"><img src="https://static.igem.org/mediawiki/2017/6/66/T--CSMU_NCHU_Taiwan--see_more.png" alt=""style="cursor: pointer ;width:120px"></a> | <a href="http://parts.igem.org/Part:BBa_K2382013" target="_blank"><img src="https://static.igem.org/mediawiki/2017/6/66/T--CSMU_NCHU_Taiwan--see_more.png" alt=""style="cursor: pointer ;width:120px"></a> |
Revision as of 21:15, 1 November 2017
Medal Criteria
This year iGEM CSMU_NCHU debuts the iGEM competition, completing all the requirements of the iGEM medal criteria, as listed below.
Bronze
Silver
Gold
- Registered for iGEM, planned to attend the Giant Jamboree
- Documented our project
- Attributed our work correctly>
- Created and documented Parts page on the Registry
- Submitted DNA samples to the Registry
- Completed safety forms about our lab, about our project and our final safety form
- Completed the judging form
- Complete interlab study
- Integrated Human Practices
- Improve previous part or project:
We improved a T7 promtore with RBS by adding a sequence in it.
We also modified the widely used RFP by removing the barcode after its sequence, and did codon optimization for E.coli as a better part for fusion protein. - Model our project:We did protein structure simulation and molecular docking to assist our fusion protein design. And moleded its expression quantity with time.
- Demonstrate: We successfully engineered a fusion protein Thioredoxin-MSMEG5998 that worked better than MSMEG_5998 alone, and proved its degrading ability in room & body temperature.