Yasmin1304 (Talk | contribs) |
Yasmin1304 (Talk | contribs) |
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</table> | </table> | ||
<p style="font-size: 20px;margin-left:40px; margin-right40px;">2. Averaging and normalizing the fluorescence readings measured for our blank and two constructs. </p> | <p style="font-size: 20px;margin-left:40px; margin-right40px;">2. Averaging and normalizing the fluorescence readings measured for our blank and two constructs. </p> | ||
+ | |||
+ | <table style="width:50%"> | ||
+ | |||
+ | <tr> | ||
+ | <th></th> | ||
+ | <th></th> | ||
+ | <th>0mM</th> | ||
+ | <th>50mM</th> | ||
+ | <th>100mM</th> | ||
+ | <th>200mM</th> | ||
+ | <th>400mM</th> | ||
+ | <th>800mM</th> | ||
+ | <th>1600mM</th> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td rowspan="3">Average </td> | ||
+ | <td>NFkB</td> | ||
+ | <td>324239</td> | ||
+ | <td>321849.33</td> | ||
+ | <td>323644.33</td> | ||
+ | <td>324455</td> | ||
+ | <td>327717.7</td> | ||
+ | <td>415060.7</td> | ||
+ | <td>430193.3 </td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | |||
+ | <td>Consensus</td> | ||
+ | <td>325839.33</td> | ||
+ | <td>325792</td> | ||
+ | <td>325800.33</td> | ||
+ | |||
+ | <td>328046.7</td> | ||
+ | <td>336568.3</td> | ||
+ | <td>369599.7</td> | ||
+ | <td>383859.7</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> Wildtype</td> | ||
+ | <td>322429</td> | ||
+ | <td>323656</td> | ||
+ | <td>326336.33</td> | ||
+ | <td>324749.3</td> | ||
+ | |||
+ | <td>331049.3</td> | ||
+ | <td>354069.7</td> | ||
+ | <td>402995</td> | ||
+ | |||
+ | </tr> | ||
+ | <tr> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td rowspan="2">NFkB</td> | ||
+ | <th>Consensus</th> | ||
+ | <th>1600.3333</th> | ||
+ | <th>3942.6667</th> | ||
+ | <th>2156</th> | ||
+ | <th>3591.667</th> | ||
+ | <th>8850.667</th> | ||
+ | <th>-45461</th> | ||
+ | <th>-46333.7 </th> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>WildType</td> | ||
+ | <td>-1810</td> | ||
+ | <td>1806.6667</td> | ||
+ | <td>2692</td> | ||
+ | <td>294.3333</td> | ||
+ | <td>3331.667</td> | ||
+ | <td>-60991</td> | ||
+ | <td>-27198.3</td> | ||
+ | |||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </table> | ||
<p style="font-size: 20px;margin-left:40px; margin-right40px;">3. The normalized fluorescence values for the two constructs at different NaCl concentrations. | <p style="font-size: 20px;margin-left:40px; margin-right40px;">3. The normalized fluorescence values for the two constructs at different NaCl concentrations. |
Revision as of 01:27, 2 November 2017
BioSensor Lab Results
As we were unable to transform the construct, therefore, the results for Salt Sensor testing were obtained from iGEM Pitt as a part of the collaboration
1. The results for the RFP fluorescence measured for three replicated of the blank as well as two constructs at excitation 585nm/emission 607nm.
NFkB (negative control) | 0mM | 50mM | 100mM | 200mM | 400mM | 800mM | 1600mM |
---|---|---|---|---|---|---|---|
1 | 326525 | 322701 | 324569 | 324359 | 329257 | 424215 | 447835 |
2 | 324213 | 321232 | 323679 | 324437 | 326850 | 421070 | 436049 |
3 | 321979 | 321615 | 322685 | 324569 | 327046 | 399897 | 406696 |
Wildtype | 0mM | 50mM | 100mM | 200mM | 400mM | 800mM | 1600mM |
1 | 322379 | 324207 | 326546 | 324794 | 330924 | 341689 | 388728 |
2 | 322539 | 323331 | 326077 | 324704 | 331310 | 367254 | 438918 |
3 | 322369 | 323430 | 326386 | 324750 | 330914 | 353266 | 381339 |
Consensus | 0mM | 50mM | 100mM | 200mM | 400mM | 800mM | 1600mM |
1 | 326742 | 326016 | 325673 | 329309 | 337418 | 377574 | 378246 |
2 | 325087 | 326368 | 326416 | 327722 | 337804 | 367197 | 400206 |
3 | 325689 | 324992 | 325312 | 327109 | 334483 | 364028 | 373127 |
2. Averaging and normalizing the fluorescence readings measured for our blank and two constructs.
0mM | 50mM | 100mM | 200mM | 400mM | 800mM | 1600mM | ||
---|---|---|---|---|---|---|---|---|
Average | NFkB | 324239 | 321849.33 | 323644.33 | 324455 | 327717.7 | 415060.7 | 430193.3 |
Consensus | 325839.33 | 325792 | 325800.33 | 328046.7 | 336568.3 | 369599.7 | 383859.7 | |
Wildtype | 322429 | 323656 | 326336.33 | 324749.3 | 331049.3 | 354069.7 | 402995 | |
NFkB | Consensus | 1600.3333 | 3942.6667 | 2156 | 3591.667 | 8850.667 | -45461 | -46333.7 |
WildType | -1810 | 1806.6667 | 2692 | 294.3333 | 3331.667 | -60991 | -27198.3 |
3. The normalized fluorescence values for the two constructs at different NaCl concentrations.