Difference between revisions of "Team:Northwestern/Safety"
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<p class="body-cont w4-center" style="font-family: josefin-sans, sans-serif; font-size:22px"> In addition to completing iGEM's safety requirements through boot-camp training, every member of our team also had to go through Northwestern's safety modules, NSIS. Our team received safety advice from our professors and graduate students throughout our summer process. A variety of safety issues arise when considering packaging applications of genome editing proteins. </p> | <p class="body-cont w4-center" style="font-family: josefin-sans, sans-serif; font-size:22px"> In addition to completing iGEM's safety requirements through boot-camp training, every member of our team also had to go through Northwestern's safety modules, NSIS. Our team received safety advice from our professors and graduate students throughout our summer process. A variety of safety issues arise when considering packaging applications of genome editing proteins. </p> | ||
| − | <h1 style= "font-family:aladin, sans-serif; font-size: 30 px" class="w3-center"> Cas9 issues </h1> | + | <h1 style= "font-family:aladin, sans-serif; font-size: 30 px; color: grey" class="w3-center"> Cas9 issues </h1> |
<p class="body-cont w4-center" style="font-family: josefin-sans, sans-serif; font-size:22px"> Off target effects are always a concern when working with gene editing. Undesirable DNA cleavage can cause fatal effects for a cell and negate the efficacy of treatment. However, the usage of OMV transport reduces off target effects as it ensures that uptake will only occur within a bacterial cell. Additionally, OMVs are preferable over viral transport due to OMVs ability to deliver whole proteins. Whole proteins are not incorporated in the host cell's genome, thereby minimizing off target risks. <br> <br> | <p class="body-cont w4-center" style="font-family: josefin-sans, sans-serif; font-size:22px"> Off target effects are always a concern when working with gene editing. Undesirable DNA cleavage can cause fatal effects for a cell and negate the efficacy of treatment. However, the usage of OMV transport reduces off target effects as it ensures that uptake will only occur within a bacterial cell. Additionally, OMVs are preferable over viral transport due to OMVs ability to deliver whole proteins. Whole proteins are not incorporated in the host cell's genome, thereby minimizing off target risks. <br> <br> | ||
Revision as of 20:59, 29 August 2017
Saftey
Science innovation comes from the intersection of practical and theoretical application. Scientists have to consider the ethical implications of their work when solving the issues of today.
The 2017 NU iGEM team made safety a priority throughout the experience and considered ethics, public safety, and risks.
Safety concerns
In addition to completing iGEM's safety requirements through boot-camp training, every member of our team also had to go through Northwestern's safety modules, NSIS. Our team received safety advice from our professors and graduate students throughout our summer process. A variety of safety issues arise when considering packaging applications of genome editing proteins.
Cas9 issues
Off target effects are always a concern when working with gene editing. Undesirable DNA cleavage can cause fatal effects for a cell and negate the efficacy of treatment. However, the usage of OMV transport reduces off target effects as it ensures that uptake will only occur within a bacterial cell. Additionally, OMVs are preferable over viral transport due to OMVs ability to deliver whole proteins. Whole proteins are not incorporated in the host cell's genome, thereby minimizing off target risks.
Effective design of guide RNA (gRNA) will also minimize off target effects.
