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+ | <br> | ||
+ | <br> | ||
+ | <h3>Experiment</h3> | ||
+ | <p class="main"> | ||
+ | The colonies was observed from blue light box. | ||
+ | The controls show that the plasmid containing GFP gene will give fluorescence. | ||
+ | <br> | ||
+ | <br> | ||
+ | The strength of promoter is correlated to the strength of promoter. The higher the strength, the higher the expression of GFP. Among the colonies from devices 4-6, the colonies from device 4 show the highest fluorescence while those from device 6 show the lowest. | ||
+ | Among the colonies from devices 1-3, the colonies from device 3 show the lowest fluorescence but <font color="red">no significant difference</font> in fluorescence between the colonies from devices 1 and 2. | ||
+ | </p> | ||
− | </ | + | <p><img src="https://static.igem.org/mediawiki/2017/1/12/Cuhkigemcolony.PNG" style="width:400px;height:500px;" ></p> |
+ | <p><img src="https://static.igem.org/mediawiki/2017/9/9c/CuhkigemOD600graph.PNG" style="width:800px;height:400px;" ></p> | ||
+ | <br><br> | ||
+ | <p> | ||
+ | <b>Fig.1 </b>shows an increasing trend of the cell growth under OD600 absorbance and Fig.2 shows an increasing trend of the fluorescence. <br> | ||
+ | <b>Fig.3 </b> shows the trend of fluorescence per cell.<br> | ||
+ | Among devices 4-6, the data match with the fluorescence of their colonies and the strength of their promoters. Device 4 has the highest fluorescence per cell and device 6 has the lowest. Among devices 1-3, the data also match with the fluorescence of their colonies and the strength of their promoters. Device 1 has the highest fluorescence per cell and device 3 has the lowest. | ||
+ | <br><br> | ||
+ | Interestingly, the fluorescence per cell of device 6 and negative control are very close. The fluorescence per cell of device 6 is even lower than the negative control at some time points. | ||
+ | This shows that device 6 produces very little amount of GFP and the result may also be affected by the measurement error in plate reader. | ||
+ | <br><br> | ||
+ | Another interesting point is the fluorescence per cell of all devices peaked at (t=2hours). One of the possible reasons is the cells spend more energy on cell division but not on producing GFP. | ||
+ | </p> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2017/e/e9/Cuhkigemfluorescence.PNG" style="width:500px;height:300px;" ></p> | ||
+ | <br><br><br><br> | ||
+ | <p><img src="https://static.igem.org/mediawiki/2017/0/03/Cuhkigemfluoresceinconc.PNG" style="width:500px;height:300px;" ></p> | ||
+ | |||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | <p> | ||
+ | Reference<br> | ||
+ | 1. Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D. Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. | ||
+ | |||
+ | </p> | ||
+ | |||
+ | </div> | ||
</html> | </html> | ||
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Revision as of 12:35, 22 September 2017