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| − | <a href="https://2017.igem.org/Team:TMMU-China" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Home <b class="fa fa-caret-down"></b></a> | + | <a href="https://2017.igem.org/Team:TMMU-China" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Home <b class="fa fa-caret-down"></b></a> |
| − | <ul class="dropdown-menu agile_short_dropdown"> | + | <ul class="dropdown-menu agile_short_dropdown"> |
| − | <li><a href="https://2017.igem.org/Team:TMMU-China">Abstract</a></li> | + | <li><a href="https://2017.igem.org/Team:TMMU-China">Abstract</a></li> |
| − | <li><a href="#">Background</a></li> | + | <li><a href="#">Background</a></li> |
| − | </ul>
| + | </ul> |
| − | </li>
| + | </li> |
| | | | |
| − | <li class="dropdown">
| + | <li class="dropdown"> |
| − | <a href="https://2017.igem.org/Team:TMMU-China/Team" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Team<b class="fa fa-caret-down"></b></a> | + | <a href="https://2017.igem.org/Team:TMMU-China/Team" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Team<b class="fa fa-caret-down"></b></a> |
| − | <ul class="dropdown-menu agile_short_dropdown"> | + | <ul class="dropdown-menu agile_short_dropdown"> |
| − | <li><a href="https://2017.igem.org/Team:TMMU-China/Team">Team</a></li> | + | <li><a href="https://2017.igem.org/Team:TMMU-China/Team">Team</a></li> |
| − | <li><a href="#">Collaborations</a></li> | + | <li><a href="#">Collaborations</a></li> |
| | | | |
| − | </ul>
| + | </ul> |
| − | </li>
| + | </li> |
| − |
| + | |
| − | <li class="dropdown">
| + | <li class="dropdown"> |
| − | <a href="#" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Project<b class="fa fa-caret-down"></b></a> | + | <a href="#" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Project<b class="fa fa-caret-down"></b></a> |
| − | <ul class="dropdown-menu agile_short_dropdown"> | + | <ul class="dropdown-menu agile_short_dropdown"> |
| − | <li><a href="#">Description</a></li> | + | <li><a href="#">Description</a></li> |
| − | <li><a href="#">Design</a></li> | + | <li><a href="#">Design</a></li> |
| − | <li><a href="#">Experiments</a></li> | + | <li><a href="#">Experiments</a></li> |
| − | <li><a href="#">Notebook</a></li> | + | <li><a href="#">Notebook</a></li> |
| − | <li><a href="#">InterLab</a></li> | + | <li><a href="#">InterLab</a></li> |
| − | <li><a href="#">Contribution</a></li> | + | <li><a href="#">Contribution</a></li> |
| − | <li><a href="#">Model</a></li> | + | <li><a href="#">Model</a></li> |
| − | <li><a href="#">Results</a></li> | + | <li><a href="#">Results</a></li> |
| − | <li><a href="#">Demonstrate</a></li> | + | <li><a href="#">Demonstrate</a></li> |
| − | <li><a href="#">Improve</a></li> | + | <li><a href="#">Improve</a></li> |
| − | <li><a href="https://2017.igem.org/Team:TMMU-China/Attributions">Attributions</a></li> | + | <li><a href="https://2017.igem.org/Team:TMMU-China/Attributions">Attributions</a></li> |
| − | </ul>
| + | </ul> |
| − | </li>
| + | </li> |
| | | | |
| | <li class="dropdown"> | | <li class="dropdown"> |
| − | <a href="#" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Parts <b class="fa fa-caret-down"></b></a> | + | <a href="#" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Parts <b class="fa fa-caret-down"></b></a> |
| − | <ul class="dropdown-menu agile_short_dropdown"> | + | <ul class="dropdown-menu agile_short_dropdown"> |
| − | <li><a href="#">Basic Parts</a></li> | + | <li><a href="#">Basic Parts</a></li> |
| − | <li><a href="#">Composite Parts</a></li> | + | <li><a href="#">Composite Parts</a></li> |
| − | <li><a href="#">Part Collection</a></li> | + | <li><a href="#">Part Collection</a></li> |
| − | </ul>
| + | </ul> |
| − | </li>
| + | </li> |
| | | | |
| | <li><a href="#" class="hvr-underline-from-center">Safety</a></li> | | <li><a href="#" class="hvr-underline-from-center">Safety</a></li> |
| | | | |
| − | <li class="dropdown">
| + | <li class="dropdown"> |
| − | <a href="#" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Human Practices <b class="fa fa-caret-down"></b></a> | + | <a href="#" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">Human Practices <b class="fa fa-caret-down"></b></a> |
| − | <ul class="dropdown-menu agile_short_dropdown"> | + | <ul class="dropdown-menu agile_short_dropdown"> |
| − | <li><a href="#">Silver HP</a></li> | + | <li><a href="#">Silver HP</a></li> |
| − | <li><a href="#">Integrated and Gold</a></li> | + | <li><a href="#">Integrated and Gold</a></li> |
| − | <li><a href="#">Public Engagement</a></li> | + | <li><a href="#">Public Engagement</a></li> |
| − | </ul>
| + | </ul> |
| − | </li>
| + | </li> |
| | | | |
| − | <li class="dropdown">
| + | <li class="dropdown"> |
| − | <a href="#" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">AWARDS<b class="fa fa-caret-down"></b></a> | + | <a href="#" class="dropdown-toggle hvr-underline-from-center" data-toggle="dropdown">AWARDS<b class="fa fa-caret-down"></b></a> |
| − | <ul class="dropdown-menu agile_short_dropdown"> | + | <ul class="dropdown-menu agile_short_dropdown"> |
| − | <li><a href="#"> Applied Design</a></li> | + | <li><a href="#"> Applied Design</a></li> |
| − | <li><a href="#">Entrepreneurship</a></li> | + | <li><a href="#">Entrepreneurship</a></li> |
| − | <li><a href="#">Hardware</a></li> | + | <li><a href="#">Hardware</a></li> |
| − | <li><a href="#">Measurement</a></li> | + | <li><a href="#">Measurement</a></li> |
| − | <li><a href="#">Model</a></li> | + | <li><a href="#">Model</a></li> |
| − | <li><a href="#">Plant</a></li> | + | <li><a href="#">Plant</a></li> |
| − | <li><a href="#">Software</a></li> | + | <li><a href="#">Software</a></li> |
| − | </ul>
| + | </ul> |
| − | </li>
| + | </li> |
| | | | |
| − | <li><a href="#" class="hvr-underline-from-center">JUDGING FORM</a></li> | + | <li><a href="#" class="hvr-underline-from-center">JUDGING FORM</a></li> |
| − |
| + | |
| − | </ul>
| + | </ul> |
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| − | </nav>
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| − | </div>
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| + | </nav> |
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| − | <div class="clearfix"> </div> | + | <div class="clearfix"> </div> |
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| | </div> | | </div> |
| | </div> | | </div> |
| | <!-- 轮播（Carousel）导航 --> | | <!-- 轮播（Carousel）导航 --> |
| | <a class="carousel-control left" href="#myCarousel" | | <a class="carousel-control left" href="#myCarousel" |
| − | data-slide="prev">‹ | + | data-slide="prev">‹ |
| | </a> | | </a> |
| | <a class="carousel-control right" href="#myCarousel" | | <a class="carousel-control right" href="#myCarousel" |
| − | data-slide="next">› | + | data-slide="next">› |
| | </a> | | </a> |
| | </div> | | </div> |
| − | <div class="banner-bottom"> | + | <div class="banner-bottom"> |
| − | | + | |
| − | <div class="mid_agile_bannner_top_info"> | + | <div class="mid_agile_bannner_top_info"> |
| − | <h1><span>Abstract</span></h1>
| + | <h1><span>Abstract</span></h1> |
| − | <div class="heading-underline"> | + | <div class="heading-underline"> |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <div class="clearfix"></div> | + | <div class="clearfix"></div> |
| − | </div>
| + | </div> |
| − |
| + | |
| − | | + | |
| − | <h3>Development of Quorum Sensing Tool Kit for <i>Lactococcus lactis</i></h3>
| + | <h3>Development of Quorum Sensing Tool Kit for Gram-positive Bacteria</h3> |
| − | <p>Gram-positive bacteria comprise various kinds of microbes, among which, <i>Staphylococcus aureus, Streptococcus pneumonia and Mycobacterium tuberculosis</i> can cause a lot of human infections, while <i>Bacillus subtilis</i> and <i>Lactococcus lactis</i> can be used as probiotics, and both of them are also widely used in synthetic biology. Quorum sensing (QS) system can play diverse roles in response to bacteria population density, which makes it an intriguing tool for synthetic biologists. The QS systems have been applied in improving the function of self-organizing networks and synchronizing genetic clocks, etc. These work mostly relied on the N-acyl homoserine lactones (AHLs) based QS tool kit of Gram-negative bacteria, however, QS tool kit for Gram-positive bacteria has rarely reported. In this project, we want to develope a QS tool kit for Gram-positive bacteria. We will build this tool kit based on the Agr system from <i>S.aureus</i>, the PlcR-PapR system from <i>Bacillus cereus</i>, and the AimR-AimP system from the <i>Bacillus subtilis</i> bacteriophage Phi3T. We are going to test the utility of this tool kit in <i>Bacillus subtilis</i> and <i>Lactococcus lactis</i>. The orthogonality of these systems will also be examined through using the Sender-Receiver pair. This QS tool kit will facilitate synthetic biologists to construct more sophisticated systems, both in Gram-positive bacteria and mixed microbial consortia. </p> | + | <p align="left">Gram-positive bacteria comprise various kinds of microbes, among which, <i>Staphylococcus aureus, Streptococcus pneumonia and Mycobacterium tuberculosis</i> can cause a lot of human infections, while <i>Bacillus subtilis</i> and <i>Lactococcus lactis</i> can be used as probiotics, and both of them are also widely used in synthetic biology. Quorum sensing (QS) system can play diverse roles in response to bacteria population density, which makes it an intriguing tool for synthetic biologists. The QS systems have been applied in improving the function of self-organizing networks and synchronizing genetic clocks, etc. These work mostly relied on the N-acyl homoserine lactones (AHLs) based QS tool kit of Gram-negative bacteria, however, QS tool kit for Gram-positive bacteria has rarely reported. In this project, we want to develope a QS tool kit for Gram-positive bacteria. We will build this tool kit based on the Agr system from <i>S.aureus</i>, the PlcR-PapR system from <i>Bacillus cereus</i>, and the AimR-AimP system from the <i>Bacillus subtilis</i> bacteriophage Phi3T. We are going to test the utility of this tool kit in <i>Bacillus subtilis</i> and <i>Lactococcus lactis</i>. The orthogonality of these systems will also be examined through using the Sender-Receiver pair. This QS tool kit will facilitate synthetic biologists to construct more sophisticated systems, both in Gram-positive bacteria and mixed microbial consortia. </p> |
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| | <div class="container"> | | <div class="container"> |
| − | <div class="row clearfix"> | + | <div class="row clearfix"> |
| − | <div class="col-md-4 column"> | + | <div class="col-md-4 column"> |
| − | <img width="300px" src="https://static.igem.org/mediawiki/2017/f/f1/T--TMMU-China--tmmu.png" /> | + | <img width="280px" src="https://static.igem.org/mediawiki/2017/f/f1/T--TMMU-China--tmmu.png" /> |
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| − | <div class="col-md-4 column"> | + | <div class="col-md-4 column"> |
| − | <img width="300px" src="https://static.igem.org/mediawiki/2017/9/91/T--TMMU-China--jcb.png" /> | + | <img width="280px" src="https://static.igem.org/mediawiki/2017/9/91/T--TMMU-China--jcb.png" /> |
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| − | <div class="col-md-4 column"> | + | <div class="col-md-4 column"> |
| | <p style="font-family:'Open Sans', sans-serif">Contact us</br> | | <p style="font-family:'Open Sans', sans-serif">Contact us</br> |
| − | Email:& #160;<a href="Mailto:igem@tmmu.edu.cn"style="color:#000">igem@tmmu.edu.cn</a></br> | + | Email:& nbsp;<a href="Mailto:igem@tmmu.edu.cn"style="color:#000">igem@tmmu.edu.cn</a></br> |
| | Address:Third Military Medical University, </br>No.30 Gaotanyan Street Shapingba District,</br> Chongqing, P.R.China 400038 | | Address:Third Military Medical University, </br>No.30 Gaotanyan Street Shapingba District,</br> Chongqing, P.R.China 400038 |
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