Difference between revisions of "Team:Lambert GA/Hardware"

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Revision as of 11:26, 25 October 2017


Description


The Lambert iGEM team is attempting to address two themes that embody synthetic biology and its diverse range of applications: precision and universality. Last year, the 2016 Lambert iGEM team attempted to address the issue of overexpression of proteins by devising a “switch”, a genetically engineered construct that degraded GFP (green fluorescent protein) using a protease mechanism ClpXP upon induction of IPTG. This year’s team is continuing to build upon this idea of characterizing ClpXP by further developing our genetic construct via the use of various chromoproteins and promoters. The data will be quantified using the ChromQ, a camera device that standardizes the light source to accurately measure the amount of chromoprotein present in a pellet of cells. Our ChromQ aims to allow especially under-funded labs to have access to a device that quantifies data without spending thousands of dollars; in addition, a functional app and mathematical model are being created to be able to compare expressions of pigments before, during, and after induction of IPTG. Ultimately, the 2017 Lambert iGEM team is striving to further characterize a precise, non-lysosomal induced protein degradation (ClpXP) and to market an affordable device (ChromQ) that can be universally used to quantify data.




















Improving Previous Parts








References

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Baker, T. A., & Sauer, R. T. (2011, June 27). ClpXP, an ATP-powered unfolding and protein-degradation machine. Retrieved Summer, 2017, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209554/

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Bohn , C. , Binet , E. , and Bouloc , P. ( 2002 ) Screening for stabilization of proteins with a trans-translation signature in Escherichia coli selects for inactivation of the ClpXP protease . Mol Genet Genomics 266 : 827 –831 .

Burton , R.E. , Siddiqui , S.M. , Kim , Y.I. , Baker , T.A. , and Sauer , R.T. ( 2001 ) Effects of protein stability and structure on substrate processing by the ClpXP unfolding and degradation machine . EMBO J 20 : 3092 –3100 .

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