Difference between revisions of "Team:Dalhousie/Improve"

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<h1><font color= "#C1D35D">Part Improvement</font></h1></br>
 
<h1><font color= "#C1D35D">Part Improvement</font></h1></br>
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<font color= "#C1D35D">Background</font>
 
<font color= "#ffffff">This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax  
 
<font color= "#ffffff">This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax  
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(<a href="http://parts.igem.org/Part:BBa_K2160000">BBa_K2160000</a>). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose.</font>
+
(<a href="http://parts.igem.org/Part:BBa_K2160000">BBa_K2160000</a>). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose.</font></br>
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<font color= "#C1D35D">Improvement</font>
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<font color= "#ffffff">We improved the endoglucanase part by adding a C-terminal HIS-tag and a N-terminal PelB sequence. The C-terminal HIS-tag allows identification via western blot or immuno-fluorescence, and protein purification. The PelB sequence is a localization sequence that traffics the protein to the periplasm (Sockolosky & Szoka, 2013). This is especially important for our project because we need to get all the enzymes out of the E. coli to digest cellulose.</font></br>
 
http://parts.igem.org/Part:BBa_K2331000      LINKKK
 
http://parts.igem.org/Part:BBa_K2331000      LINKKK
  

Revision as of 00:37, 27 October 2017

Improve

Part Improvement


Background This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax (BBa_K2160000). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose.
Improvement We improved the endoglucanase part by adding a C-terminal HIS-tag and a N-terminal PelB sequence. The C-terminal HIS-tag allows identification via western blot or immuno-fluorescence, and protein purification. The PelB sequence is a localization sequence that traffics the protein to the periplasm (Sockolosky & Szoka, 2013). This is especially important for our project because we need to get all the enzymes out of the E. coli to digest cellulose.
http://parts.igem.org/Part:BBa_K2331000 LINKKK

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