MackenzieRT (Talk | contribs) |
MackenzieRT (Talk | contribs) |
||
Line 199: | Line 199: | ||
<h1><font color= "#C1D35D">Part Improvement</font></h1></br> | <h1><font color= "#C1D35D">Part Improvement</font></h1></br> | ||
− | <font color= "#C1D35D">Background</font> | + | |
+ | <font color= "#C1D35D">Background</font></br> | ||
+ | |||
<font color= "#ffffff">This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax | <font color= "#ffffff">This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax | ||
(<a href="http://parts.igem.org/Part:BBa_K2160000">BBa_K2160000</a>). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose.</font></br> | (<a href="http://parts.igem.org/Part:BBa_K2160000">BBa_K2160000</a>). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose.</font></br> | ||
− | <font color= "#C1D35D">Improvement</font> | + | |
+ | <font color= "#C1D35D">Improvement</font></br> | ||
+ | |||
<font color= "#ffffff">We improved the endoglucanase part by adding a C-terminal HIS-tag and a N-terminal PelB sequence. The C-terminal HIS-tag allows identification via western blot or immuno-fluorescence, and protein purification. The PelB sequence is a localization sequence that traffics the protein to the periplasm (Sockolosky & Szoka, 2013). This is especially important for our project because we need to get all the enzymes out of the E. coli to digest cellulose.</font></br> | <font color= "#ffffff">We improved the endoglucanase part by adding a C-terminal HIS-tag and a N-terminal PelB sequence. The C-terminal HIS-tag allows identification via western blot or immuno-fluorescence, and protein purification. The PelB sequence is a localization sequence that traffics the protein to the periplasm (Sockolosky & Szoka, 2013). This is especially important for our project because we need to get all the enzymes out of the E. coli to digest cellulose.</font></br> | ||
http://parts.igem.org/Part:BBa_K2331000 LINKKK | http://parts.igem.org/Part:BBa_K2331000 LINKKK |
Revision as of 00:38, 27 October 2017
Improve
Part Improvement
Background This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax (BBa_K2160000). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose. Improvement We improved the endoglucanase part by adding a C-terminal HIS-tag and a N-terminal PelB sequence. The C-terminal HIS-tag allows identification via western blot or immuno-fluorescence, and protein purification. The PelB sequence is a localization sequence that traffics the protein to the periplasm (Sockolosky & Szoka, 2013). This is especially important for our project because we need to get all the enzymes out of the E. coli to digest cellulose. http://parts.igem.org/Part:BBa_K2331000 LINKKKLearn more... hopefully have links to next pages here