MackenzieRT (Talk | contribs) |
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<font color= "#ffffff">This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax | <font color= "#ffffff">This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax | ||
− | (<a href="http://parts.igem.org/Part:BBa_K2160000" | + | (<a href="http://parts.igem.org/Part:BBa_K2160000" style="color: #C1D35D">BBa_K2160000</a>). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose.</font></br> |
<font color= "#C1D35D">Improvement</font></br> | <font color= "#C1D35D">Improvement</font></br> |
Revision as of 00:43, 27 October 2017
Improve
Part Improvement
Background This part is the coding region for an endoglucanse from Ruminiclostridium thermocellum that was previously created by the iGEM16_Dalhousie_NS_Halifax (BBa_K2160000). Its function is to cleave internal Beta-1,4-D-glycosidic bonds in the cellulose crystal to release the disaccharide cellobiose. Improvement We improved the endoglucanase part by adding a C-terminal HIS-tag and a N-terminal PelB sequence. The C-terminal HIS-tag allows identification via western blot or immuno-fluorescence, and protein purification. The PelB sequence is a localization sequence that traffics the protein to the periplasm (Sockolosky & Szoka, 2013). This is especially important for our project because we need to get all the enzymes out of the E. coli to digest cellulose. http://parts.igem.org/Part:BBa_K2331000 LINKKKLearn more... hopefully have links to next pages here