Difference between revisions of "Team:Tianjin/Engagement"

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/* OVERRIDE IGEM SETTINGS */
 
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             <article id="description">       
 
             <article id="description">       
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                 <h1>Engagement</h1>
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                 <h1>Silver</h1>
 
                     <hr />
 
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<h2>Helping TUST prepare for their first year of competition.</h2>
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<h2>Engagement</h2>
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<p>To begin with, we gave them construct suggestions, communicated with their team adviser. In 24/3/2017, we were invited to TUST to carried out recruiting propaganda for them, and provided our construction guide.</p>
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<p> During these months，we have done some activities that involved all parts of people in society to increase people's knowledge about Synthetic Biology, to make iGEM known to more people and to make our project more influential among people of various jobs. In order to achieve the first goal, we took use of the platform of Begonia Festival of our school to hold up a propaganda involving intriguing games. For the second goal, we set up a Lab-Open day which allows high school students, college students and adults to visit our labs and do some easy experiments in person and to take part in the teach-ins held by our team to know more details of iGEM. We also went to TUST to take part in their iGEM promotion activities to increase the popularity of iGEM in their school and to promote the amicable relationships between our team and team of TUST.</p>
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                            <img src="https://static.igem.org/mediawiki/2017/5/54/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171003130153.jpg"></a>
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                  <div id="pic_seven" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/5/54/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171003130153.jpg"/></div>
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<h3>2017.08.01</h3>
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<h3>more</h3>
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<p>In 2017.08.01, TUST and Tianjin held a meeting together, during the meeting, TUST gave an account of their recent progress, we suggested them to add some new synthetic routes and elements, and probably new strain instead of simply Xylinus for fibrin.</p>
+
<p>I What's more, we developed a public research involving people across China on the platform of Wechat for the sake of the third goal.</p>
 
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<h3>Later on</h3>
 
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<p>Later on, TUST came again with their modeling problem, we gave them suggestion that we can share the Fluorescence method and modeling method with them, design Fluorescence experience and modeling together since it’s their first year competition.</p>
 
  
  
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<h2>Begonia Festival</h2>
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<p> On April 3rd, about thirty thousand people stepped in TianJin University to take part in the Begonia Festival and to enjoy the bueaty of spring. Our team decided to take advantage of this opportunity to make public know more about Synthetic Biology. We let people joining our activities inoculate bacterias in culture medium in any pattern they want, during which they can enjoy the happiness of doing biology experiments and get more interested in biology study.</p>
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                  <div id="pic_one" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/9/9c/692217429000843970.jpg"/></div>
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                            <img src="https://static.igem.org/mediawiki/2017/d/d8/Tianjin2.jpg"/>
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                  <div id="pic_three" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/c/cf/291519661563138743.jpg"/></div>
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                  <div id="pic_four" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/d/d8/Tianjin2.jpg"/></div>
  
  
  
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<h2>Helping NKU with their plasmid construction</h2>
 
−
<p>1. NKU asked us to help them construct a plasmid. Using pEX18Gm as the supporter, we ought to added Lyase gene sequence（which they had already provided） and xyR-xyA sequence from pAX01 as well.</p>
 
 
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<h3>After construction</h3>
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<h3>After</h3>
−
<p>After construction, they sent us plasmid pAX01 and the plasmid which contains Lyase gene sequence, we helped them with ligasion and transformation.</p>
+
<p> A few days later, when the bacteria they inoculated grew mature, we put these pictures of the pattern bacterias grew onto our Wechat subscription, which received a good feedback among people who joined it. </p>
 
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                            <img src="https://static.igem.org/mediawiki/2017/f/f7/896358115638089642.jpg"></a>
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                            <img src="https://static.igem.org/mediawiki/2017/0/00/495808992225298876.jpg"/>
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                  <div id="pic_five" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/f/f7/896358115638089642.jpg"/></div>
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<h2>Test the mini system for OUC</h2>
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<h2>Lab-Open Day</h2>
−
<p>Mini system is a system composed of a mini promoter (about 100bp) and a mini terminator, and it is applicable for yeast. Because of the similarity of our biological chassis, they reached out to us and wanted us to run some control experiments for them. Using the same protocol and their mini system, we need to transform the system into our Saccharomyces cerevisiae in order to test if the plasmid will still function the same. </p>
+
<p>In order to promote the popularity of iGEM and Synthetic Biology among people who are interested in biology. We released the information of this activity in our Wechat subscription and received many entry blanks from high school students, college students and the parents of this high school students. First of all, we held a teach-ins taken part by these students and parents. During the meeting, we talked about what is iGEM, its origin and rules. What is more, we talked about the projects of TianJin iGEM in recent years with easy- to-understand way. In the end of the meeting, there was a question-answer session, which means that we threw out some questions based on the contents of we had talked, the student with the highest score can abstain our special gifts. By this session, we knew that students joined our meeting already had a rough understand of iGEM and Synthetic Biology.</p>
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<div class="zxx_zoom_left">
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                    <div class="small_pic" style="float:left;">
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                        <a href="#pic_eight">
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                            <img src="https://static.igem.org/mediawiki/2017/2/26/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213351.jpg"></a>
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<div style="padding-left:20%;"><p></p></div>
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                        <a href="#pic_nine" >
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                            <img src="https://static.igem.org/mediawiki/2017/5/5c/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213355.jpg"/>
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                        </a>
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                  <div id="pic_eight" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/2/26/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213351.jpg"/></div>
 +
                  <div id="pic_nine" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/5/5c/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213355.jpg"/></div> 
  
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<h3>Protocol</h3>
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−
<p>2. Protocol: initial OD600 = 0.01
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<h3>after meeting</h3>
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        Test OD600 and threshold fluorescence every other 5-6 hours
+
<p>After the meeting, we took them to our labs. We showed them the experimental process of Synthetic Biology such as PCR, inoculate bacterias on the culture medium, centrifugation and extract plasmid. By this visit, both students and parents got a closer understand of biology science and the process of doing scientific research. Because these parents told us that they were concerned about the prospect of doing scientific research and they were afraid of the tedium of science, we gave them some information about our experience during taking part in iGEM, and told them that doing scientific research was not as scary as they imagine.
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            Exciting light λ=502/Emissive light λ=532
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        <li><img src="https://static.igem.org/mediawiki/2017/3/35/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171003144102.jpg"/></li>
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                            <img src="https://static.igem.org/mediawiki/2017/c/ca/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213338.jpg"></a>
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        <li><img src="https://static.igem.org/mediawiki/2017/b/b4/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171003144056.jpg"></li>
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<div style="padding-left:20%;"><p></p></div>
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        <li><img src="https://static.igem.org/mediawiki/2017/3/35/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171003144102.jpg"/></li>
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                    </div>
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    </ul>
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<script type="text/javascript" src="https://2017.igem.org/Team:Tianjin/Resources/JS:index2?action=raw&ctype=text/javascript"></script> 
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                        <a href="#pic_eleven" >
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<script  type="text/javascript" src="https://2017.igem.org/Team:Tianjin/Resources/JS:jqueryminjs?action=raw&ctype=text/javascript"></script> 
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                            <img src="https://static.igem.org/mediawiki/2017/1/15/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024214841.jpg"/>
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<script  type="text/javascript" src="https://2017.igem.org/Team:Tianjin/Resources/JS:photo?action=raw&ctype=text/javascript"></script>
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                        </a>
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                  <div id="pic_eleven" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/1/15/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024214841.jpg"/></div>   
  
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<h2>Sent a Collaboration Request and construct an alliance to build a worldwide database.</h2>
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                    <div class="small_pic" style="float:left;">
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<p>We came up with the idea that we could gather all the iGEM team whose project is about water pollution treatment together and build an alliance to unite all our social impact, knowledge and geographical advantages.</p>
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                            <img src="https://static.igem.org/mediawiki/2017/8/80/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213343.jpg"></a>
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<div style="padding-left:20%;"><p></p></div>
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                        <a href="#pic_thirteen" >
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                            <img src="https://static.igem.org/mediawiki/2017/c/cd/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213347.jpg"/>
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                        </a>
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                    </div>
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                    </div>
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                  <div id="pic_twelve" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/8/80/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213343.jpg"/></div>
 +
                  <div id="pic_thirteen" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/c/cd/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171024213347.jpg"/></div> 
  
−
<h3>2017.08.12</h3>
 
−
<p>2. In 12th of August, we had a voice conferencing with SJTU/SCUT/XMU/UCAS/JLU/FAFU, we discussed about how we want to use this alliance and came up with 2 conclusion:
 
−
1. Build a worldwide database containing contents of heavy metals in soil
 
−
2. Mutual improve our social impact
 
−
</p>
 
  
 
 
−
<h3>2017.08.23</h3>
+
<h2>Public Research </h2>
−
<p>In 23th of August, we sent a Collaboration Request to iGEM official website, the next day Ana Sifuentes replied our message and posted our request in the iGEM official website, and we received the response of many teams like team EXETER and team CSMU NCHU TAIWAN, with their information, we construct a database which contains the worldwide data of contents of Cu2+/Cd2+ in soil or water. And we build it based on map of the world.</p>
+
<p>In order to know whether people around China know about pollution of heavy mental and make information about this problem known to more people, we developed a public research on the platform of our Wechat subscription. This questionnaire was filled by people in various province of China, and we can get the information that most people have a rough understand of heavy mental pollution but few of them start a research about how is the heavy mental discharged into the environment and how to reduce this pollution. According to the results of the questionnaire, we also knew that people are more willing to use biology method to settle heavy mental down than using chemical or physical method. </p>
  
−
    <div id="four" name="four">
+
 +
<p>Our research had also published on the iGEM official collaboration website. We had received 1000 answers in roll.</p>
  
−
</div>
 
−
<h2>Helping CQU construct their team and sign up for their first year</h2>
 
−
<p>First, I believe that this nation should commit itself to achieving the goal, before this decade is out, of landing a man on the moon and returning him safely to the earth. No single space project in this period will be more impressive to mankind, or more important for the long-range exploration of space; and none will be so difficult or expensive to accomplish. </p>
 
  
−
<h3>This one goes deeper</h3>
+
−
<p>A good rule for rocket experimenters to follow is this: always assume that it will explode. The vehicle explodes, literally explodes, off the pad. The simulator shakes you a little bit, but the actual liftoff shakes your entire body and soul.</p>
+
<h3>contact with experts</h3>
 +
<p>Apart from the activities we have done above, we also have a good contact with experts in Synthetic Biology to obtain more neoteric ideas. On March 7th, the founder of SynBioBeta-John Cumbers came to TianJin University to have a talk with our mentor-professor Yingjin Yuan to talk about the development of Synthetic Biology in China and the projects of our team in recent years. This meeting attracted a lot of students to come.  John Cumbers listened to the speech of our team leader-Mingzhe Han carefully and he said that it was amazing how big progress our team had made during these years. During the meeting, the investor of FressVC shared with us the broad prospects of Synthetic Biology and he told us that he was hoping us to realize the commercialization of Synthetic Biology and industrialization. In the end of the meeting, the members of our team asked some question about Synthetic Biology such as how does Synthetic Biology as a newly emerging subject realized the promotion across the world and what is the future trend of Synthetic Biology. John Cumber said that iGEM played an important part in the promotion of Synthetic Biology and in the future, Synthetic Biology may create some biological tools like computer to simulate the process of the evolvement of biological species. We learned a lot during this meeting and had a more clear assessment of Synthetic Biology.</p>
  
−
<h4>Even deeper</h4>
 
−
<p>The path of the righteous man is beset on all sides by the iniquities of the selfish and the tyranny of evil men. Blessed is he who, in the name of charity and good will, shepherds the weak through the valley of darkness, for he is truly his brother's keeper and the finder of lost children.</p>
 
  
−
<h3>And a level-3 title again</h3>
 
−
<p>It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.</p>
 
  
 +
   
 +
<div class="zxx_zoom_left" align="center">
 +
                    <div class="small_pic">
 +
                        <a href="#pic_fourteen">
 +
                            <img src="https://static.igem.org/mediawiki/2017/0/09/Qqqqqqqqqqqqqqq.jpg"></a>
 +
<div style="padding-left:20%; font-size:1.2rem;"><p>Group photo of our team and John Cumber</p></div>
 +
                    </div>
 +
                   
 +
                    </div>
 +
                  <div id="pic_fourteen" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/0/09/Qqqqqqqqqqqqqqq.jpg"/><br>Group photo of our team and John Cumber</div>
 +
                 
 +
<div id="four" name="four">
  
−
<h2>Filmed a biosafety video together with other 12 teams</h2>
+
</div>
−
<p>12 teams gather together to film a biosafety video, every team took different topics, but all based on Yale biosafety manual.</p>
+
−
 
+
−
<h3>This one goes deeper</h3>
+
−
<p>A good rule for rocket experimenters to follow is this: always assume that it will explode. The vehicle explodes, literally explodes, off the pad. The simulator shakes you a little bit, but the actual liftoff shakes your entire body and soul.</p>
+
  
−
<h4>Even deeper</h4>
 
−
<p>The path of the righteous man is beset on all sides by the iniquities of the selfish and the tyranny of evil men. Blessed is he who, in the name of charity and good will, shepherds the weak through the valley of darkness, for he is truly his brother's keeper and the finder of lost children.</p>
 
 
<div id="gobottom" name="gobottom">
 
<div id="gobottom" name="gobottom">
−
<h3>And a level-3 title again</h3>
+
</div>
−
<p>It has been said that astronomy is a humbling and character-building experience. There is perhaps no better demonstration of the folly of human conceits than this distant image of our tiny world. To me, it underscores our responsibility to deal more kindly with one another, and to preserve and cherish the pale blue dot, the only home we've ever known.</p>
+
−
</div>
+
 
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Revision as of 10:23, 27 October 2017

/* OVERRIDE IGEM SETTINGS */

Silver

Engagement

During these months，we have done some activities that involved all parts of people in society to increase people's knowledge about Synthetic Biology, to make iGEM known to more people and to make our project more influential among people of various jobs. In order to achieve the first goal, we took use of the platform of Begonia Festival of our school to hold up a propaganda involving intriguing games. For the second goal, we set up a Lab-Open day which allows high school students, college students and adults to visit our labs and do some easy experiments in person and to take part in the teach-ins held by our team to know more details of iGEM. We also went to TUST to take part in their iGEM promotion activities to increase the popularity of iGEM in their school and to promote the amicable relationships between our team and team of TUST.

more

I What's more, we developed a public research involving people across China on the platform of Wechat for the sake of the third goal.

Begonia Festival

On April 3rd, about thirty thousand people stepped in TianJin University to take part in the Begonia Festival and to enjoy the bueaty of spring. Our team decided to take advantage of this opportunity to make public know more about Synthetic Biology. We let people joining our activities inoculate bacterias in culture medium in any pattern they want, during which they can enjoy the happiness of doing biology experiments and get more interested in biology study.

After

A few days later, when the bacteria they inoculated grew mature, we put these pictures of the pattern bacterias grew onto our Wechat subscription, which received a good feedback among people who joined it.

Lab-Open Day

In order to promote the popularity of iGEM and Synthetic Biology among people who are interested in biology. We released the information of this activity in our Wechat subscription and received many entry blanks from high school students, college students and the parents of this high school students. First of all, we held a teach-ins taken part by these students and parents. During the meeting, we talked about what is iGEM, its origin and rules. What is more, we talked about the projects of TianJin iGEM in recent years with easy- to-understand way. In the end of the meeting, there was a question-answer session, which means that we threw out some questions based on the contents of we had talked, the student with the highest score can abstain our special gifts. By this session, we knew that students joined our meeting already had a rough understand of iGEM and Synthetic Biology.

after meeting

After the meeting, we took them to our labs. We showed them the experimental process of Synthetic Biology such as PCR, inoculate bacterias on the culture medium, centrifugation and extract plasmid. By this visit, both students and parents got a closer understand of biology science and the process of doing scientific research. Because these parents told us that they were concerned about the prospect of doing scientific research and they were afraid of the tedium of science, we gave them some information about our experience during taking part in iGEM, and told them that doing scientific research was not as scary as they imagine.

Public Research

In order to know whether people around China know about pollution of heavy mental and make information about this problem known to more people, we developed a public research on the platform of our Wechat subscription. This questionnaire was filled by people in various province of China, and we can get the information that most people have a rough understand of heavy mental pollution but few of them start a research about how is the heavy mental discharged into the environment and how to reduce this pollution. According to the results of the questionnaire, we also knew that people are more willing to use biology method to settle heavy mental down than using chemical or physical method.

Our research had also published on the iGEM official collaboration website. We had received 1000 answers in roll.

contact with experts

Apart from the activities we have done above, we also have a good contact with experts in Synthetic Biology to obtain more neoteric ideas. On March 7th, the founder of SynBioBeta-John Cumbers came to TianJin University to have a talk with our mentor-professor Yingjin Yuan to talk about the development of Synthetic Biology in China and the projects of our team in recent years. This meeting attracted a lot of students to come. John Cumbers listened to the speech of our team leader-Mingzhe Han carefully and he said that it was amazing how big progress our team had made during these years. During the meeting, the investor of FressVC shared with us the broad prospects of Synthetic Biology and he told us that he was hoping us to realize the commercialization of Synthetic Biology and industrialization. In the end of the meeting, the members of our team asked some question about Synthetic Biology such as how does Synthetic Biology as a newly emerging subject realized the promotion across the world and what is the future trend of Synthetic Biology. John Cumber said that iGEM played an important part in the promotion of Synthetic Biology and in the future, Synthetic Biology may create some biological tools like computer to simulate the process of the evolvement of biological species. We learned a lot during this meeting and had a more clear assessment of Synthetic Biology.

Group photo of our team and John Cumber