Difference between revisions of "Team:Chalmers-Gothenburg/Notebook/protocols"
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| − | <a href="https://static.igem.org/mediawiki/2016/e/e9/T--Chalmers_Gothenburg--Protocols_DreamTaq_PCR.pdf">Thermo Scientific DreamTaq DNA Polymerase</a><br> | + | <a href="https://static.igem.org/mediawiki/2016/e/e9/T--Chalmers_Gothenburg--Protocols_DreamTaq_PCR.pdf" target="blank">Thermo Scientific DreamTaq DNA Polymerase</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2016/c/c9/T--Chalmers_Gothenburg--Protocols_Phusion_PCR.pdf">Thermo Scientific Phusion High-Fidelity DNA Polymerase</a><br> | + | <a href="https://static.igem.org/mediawiki/2016/c/c9/T--Chalmers_Gothenburg--Protocols_Phusion_PCR.pdf" target="blank">Thermo Scientific Phusion High-Fidelity DNA Polymerase</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2017/c/c8/T--Chalmers-Gothenburg--Takara_PrimeSTAR_HS_DNA_Polymerase.pdf">PrimeSTAR HS DNA Polymerase</a><br> | + | <a href="https://static.igem.org/mediawiki/2017/c/c8/T--Chalmers-Gothenburg--Takara_PrimeSTAR_HS_DNA_Polymerase.pdf" target="blank">PrimeSTAR HS DNA Polymerase</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2017/3/36/T--Chalmers-Gothenburg--Fusion_PCR.pdf">Fusion PCR</a> | + | <a href="https://static.igem.org/mediawiki/2017/3/36/T--Chalmers-Gothenburg--Fusion_PCR.pdf" target="blank">Fusion PCR</a> |
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| − | <a href="https://static.igem.org/mediawiki/2017/8/81/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_PCR_Purification_Kit.pdf">Thermo Scientific GeneJET PCR Purification Kit</a><br> | + | <a href="https://static.igem.org/mediawiki/2017/8/81/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_PCR_Purification_Kit.pdf" target="blank">Thermo Scientific GeneJET PCR Purification Kit</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2017/8/89/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Gel_Extraction_Kit.pdf">Thermo Scientific GeneJET Gel Extraction Kit</a> | + | <a href="https://static.igem.org/mediawiki/2017/8/89/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Gel_Extraction_Kit.pdf" target="blank">Thermo Scientific GeneJET Gel Extraction Kit</a> |
</p> | </p> | ||
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| − | <a href="https://static.igem.org/mediawiki/2017/e/e9/T--Chalmers-Gothenburg--FastDigest_Restriction_Enzymes.pdf">FastDigest Restriction Enzymes</a><br> | + | <a href="https://static.igem.org/mediawiki/2017/e/e9/T--Chalmers-Gothenburg--FastDigest_Restriction_Enzymes.pdf" target="blank">FastDigest Restriction Enzymes</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2016/9/9d/T--Chalmers_Gothenburg--Protocols_gibson_assembly.pdf">Gibson Assembly Master Mix</a><br> | + | <a href="https://static.igem.org/mediawiki/2016/9/9d/T--Chalmers_Gothenburg--Protocols_gibson_assembly.pdf" target="blank">Gibson Assembly Master Mix</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2016/1/1d/T--Chalmers_Gothenburg--Protocols_general_DNA_insert_ligation.pdf">DNA Insert Ligation into Vector DNA</a> | + | <a href="https://static.igem.org/mediawiki/2016/1/1d/T--Chalmers_Gothenburg--Protocols_general_DNA_insert_ligation.pdf" target="blank">DNA Insert Ligation into Vector DNA</a> |
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| − | <a href="https://static.igem.org/mediawiki/2017/7/74/T--Chalmers-Gothenburg--Yeast_Transformation_Chemical.pdf">Transformation (Chemical)</a><br> | + | <a href="https://static.igem.org/mediawiki/2017/7/74/T--Chalmers-Gothenburg--Yeast_Transformation_Chemical.pdf" target="blank">Transformation (Chemical)</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2016/d/de/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_yeast_electroporation.pdf">Transformation (Electroporation)</a><br> | + | <a href="https://static.igem.org/mediawiki/2016/d/de/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_yeast_electroporation.pdf" target="blank">Transformation (Electroporation)</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2017/0/09/T--Chalmers-Gothenburg--Yeast_Transformation-Quick_Version.pdf">Transformation (Quick version)</a><br> | + | <a href="https://static.igem.org/mediawiki/2017/0/09/T--Chalmers-Gothenburg--Yeast_Transformation-Quick_Version.pdf" target="blank">Transformation (Quick version)</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2016/e/e2/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_quick_genomic_extraction.pdf">Isolation of genomic DNA</a> | + | <a href="https://static.igem.org/mediawiki/2016/e/e2/T--Chalmers_Gothenburg--Protocols_S_cerevisiae_quick_genomic_extraction.pdf" target="blank">Isolation of genomic DNA</a> |
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| − | <a href="https://static.igem.org/mediawiki/2016/f/f6/T--Chalmers_Gothenburg--Protocols_E_coli_transformation.pdf">Transformation (Heat shock)</a><br> | + | <a href="https://static.igem.org/mediawiki/2016/f/f6/T--Chalmers_Gothenburg--Protocols_E_coli_transformation.pdf" target="blank">Transformation (Heat shock)</a><br> |
| − | <a href="https://static.igem.org/mediawiki/2017/b/b1/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Plasmid_Miniprep_Kit.pdf">Thermo Scientific GeneJET Plasmid Miniprep Kit</a> | + | <a href="https://static.igem.org/mediawiki/2017/b/b1/T--Chalmers-Gothenburg--Thermo_Scientific_GeneJET_Plasmid_Miniprep_Kit.pdf" target="blank">Thermo Scientific GeneJET Plasmid Miniprep Kit</a> |
</p> | </p> | ||
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| − | <a href="https://static.igem.org/mediawiki/2016/c/c1/T--Chalmers_Gothenburg--Protocols_biobrick_assembly.pdf">BioBricks Assembly Protocol</a> | + | <a href="https://static.igem.org/mediawiki/2016/c/c1/T--Chalmers_Gothenburg--Protocols_biobrick_assembly.pdf" target="blank">BioBricks Assembly Protocol</a> |
</p> | </p> | ||
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| − | <a href="https://static.igem.org/mediawiki/2017/b/b4/T--Chalmers-Gothenburg--Interlab_study.pdf">Interlab study</a> | + | <a href="https://static.igem.org/mediawiki/2017/b/b4/T--Chalmers-Gothenburg--Interlab_study.pdf" target="blank">Interlab study</a> |
</p> | </p> | ||
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Revision as of 16:40, 27 October 2017
Introduction
Several protocols for specific procedures have been followed while working in the wet lab. These include protocols for PCR, purifications, digestions, assemblies as well as working with Saccharomyces cerevisiae and Escherichia coli. See the exact procedures in the lab journal.
Protocols
Protocols for PCR
Thermo Scientific DreamTaq DNA Polymerase
Thermo Scientific Phusion High-Fidelity DNA Polymerase
PrimeSTAR HS DNA Polymerase
Fusion PCR
Protocols for purification
Protocols for digestion and assembly
Protocols for Saccharomyces cerevisiae
Transformation (Chemical)
Transformation (Electroporation)
Transformation (Quick version)
Isolation of genomic DNA
Protocols for Escherichia coli
Protocols for Biobricks
Protocols for Interlab study
