Difference between revisions of "Team:Tianjin/Demonstrate"

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   <p>The yellow colony in the figure is mating successfully. After the induction of <i>galactose</i>, <i>vika recombinase</i> was expressed, and <i>red fluorescent protein</i> gene and terminator was deleted so that <i>β-carotene</i> expresses. The color of colony was changed from white to yellow. In addition to it, we also tried other methods to turn on the switch.</p>
 
   <p>The yellow colony in the figure is mating successfully. After the induction of <i>galactose</i>, <i>vika recombinase</i> was expressed, and <i>red fluorescent protein</i> gene and terminator was deleted so that <i>β-carotene</i> expresses. The color of colony was changed from white to yellow. In addition to it, we also tried other methods to turn on the switch.</p>
  
  <img>
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    <div class="zxx_zoom_demo" align="center">
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<script  type="text/javascript" src="https://static.igem.org/mediawiki/2017/7/78/Tianjin-3-Bacteria_after_mating_cultivated_on_the_Sc-His_plate.png"></script>
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                    <div class="small_pic_demo" align="center">
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                        <a href="#pic_fortyfour">
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                          <img src="https://static.igem.org/mediawiki/2017/4/4c/Tianjin-2-Three_modified_colonies_and_one_resulting_colony.jpg"></a>
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<p style="font-size:15px;text-align:center"><br/>Fig.2-1.Bacteria after matingcultivated on the Sc-His plate.</p>
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                    </div>
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                    </div>
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                  <div id="pic_fortyfour" style="display:none;"><img src="https://static.igem.org/mediawiki/2017/7/77/Tianjin-3-Bacteria_after_mating_cultivated_on_the_Sc-His_plate_yuantu.png"><br/>Fig2-1.Bacteria after matingcultivated on the Sc-His plate.</p></div>
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<p>There are 377 yellow colonies and 365 white colonies in the field of view.</p>
 
   <p>We used another α-type yeast named <i>BY4742</i>, which has a plasmid called <i>pRS413</i> with selective marker <i>His</i>. It could express <i>vika recombinase</i> before mating. It mated with a-type <i>Saccharomyces cerevisiae</i> PVRVC, and then yeast cultured on Sc-His plate. As can be seen from the figure above, the reorganization efficiency is high, which reaches 50.8 percent. This proves that our Mating switcher is fast and efficient.</p>
 
   <p>We used another α-type yeast named <i>BY4742</i>, which has a plasmid called <i>pRS413</i> with selective marker <i>His</i>. It could express <i>vika recombinase</i> before mating. It mated with a-type <i>Saccharomyces cerevisiae</i> PVRVC, and then yeast cultured on Sc-His plate. As can be seen from the figure above, the reorganization efficiency is high, which reaches 50.8 percent. This proves that our Mating switcher is fast and efficient.</p>
  

Revision as of 21:00, 27 October 2017

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Demonstrate