Difference between revisions of "Team:BostonU/HP/Silver"
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| − | <p class="inline-heading-type mainwrap">Art Project: <em> | + | <p class="inline-heading-type mainwrap">Art Project: <em>Circadia Synthetica</em></p> |
| − | <p class="body-type mainwrap indented"> | + | <p class="body-type mainwrap indented">This painted triptych is an exploration of circadian rhythms in organisms. The first half of the project takes place on Earth with naturally occurring organisms fit to the 24 hour day. The second half of the project takes place on Mars with organisms synthetically modified organisms who’ve had their circadian rhythms optimized to a Mar’s 25 hour day. The aim of this project is to display it in areas of Boston with various groups of people with different ideas and knowledge of synthetic biology. We want to get their opinions of the art anonymously to understand how far people are comfortable with the usage of synthetic modification in unusual situations. </p> |
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| − | <div class="slide" slide=" | + | <div class="slide" slide="3" annot="Sai works on the painting."> <img src="https://static.igem.org/mediawiki/2017/d/db/T--BostonU--SaiPainting2.jpg" alt="Slide 3"> </div> |
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| − | <div class="slide" slide=" | + | <div class="slide" slide="2" annot="Sai works on the painting."> <img src="https://static.igem.org/mediawiki/2017/5/5f/T--BostonU--SaiPainting1.jpg" alt="Slide 2"> </div> |
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| − | <div class="slide" slide=" | + | <div class="slide" slide="1" annot=""> <img src="https://static.igem.org/mediawiki/2017/f/ff/T--BostonU--CircadiaSynthetica.png" alt="Slide 1"> </div> |
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| − | <div class="slide" slide="5" annot=" | + | <div class="slide" slide="5" annot="The end result: the food dye separated out into its consituitive colors on the gel after 15 minutes, demonstrating how we use gel electrophoresis in our research."> <img src="https://static.igem.org/mediawiki/2017/8/81/T--BostonU--Gel.jpg" alt="Slide 5"> </div> |
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| − | <div class="slide" slide="4" annot=" | + | <div class="slide" slide="4" annot="Sai and Abbey give Summer Pathways participants instructions about lab safety and the science behind gel electrophoresis."> <img src="https://static.igem.org/mediawiki/2017/d/de/T--BostonU--Gel1.jpg" alt="Slide 4"> </div> |
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| − | <div class="slide" slide="3" annot=" | + | <div class="slide" slide="3" annot="We prepared a problem-solving exercise that simulated the process of digestion and ligation with restriction enzymes. Participants were asked to 'digest' paper plasmids with scissors at real recognition sites and 'recombine' a new genetic device."> <img src="https://static.igem.org/mediawiki/2017/f/fe/T--BostonU--Benchling2.jpg" alt="Slide 3"> </div> |
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| − | <div class="slide" slide="2" annot=" | + | <div class="slide" slide="2" annot="Stephen, Sophia, and mentor Manu lead a casual forum on the ethics and philosopy of synthetic biology now and in the future."> <img src="https://static.igem.org/mediawiki/2017/3/32/T--BostonU--Bioethics1.jpg" alt="Slide 2"> </div> |
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| − | <div class="slide" slide="1" annot=" | + | <div class="slide" slide="1" annot="A group photo of everyone involved in Summer Pathways 2017."> <img src="https://static.igem.org/mediawiki/2017/c/c5/T--BostonU--SummerPathways2.jpg" alt="Slide 1"> </div> |
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<p class="inline-heading-type mainwrap">Industry Visits</p> | <p class="inline-heading-type mainwrap">Industry Visits</p> | ||
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<div class="slide" slide="1" annot="First slide title. Photo by Forrest Cavale."> <img src="https://ununsplash.imgix.net/uploads/1413399939678471ea070/2c0343f7?q=75&fm=jpg&w=601" alt="Slide 1"> </div> | <div class="slide" slide="1" annot="First slide title. Photo by Forrest Cavale."> <img src="https://ununsplash.imgix.net/uploads/1413399939678471ea070/2c0343f7?q=75&fm=jpg&w=601" alt="Slide 1"> </div> | ||
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Revision as of 21:56, 28 October 2017
HUMAN PRACTICES
Art Project: Circadia Synthetica
This painted triptych is an exploration of circadian rhythms in organisms. The first half of the project takes place on Earth with naturally occurring organisms fit to the 24 hour day. The second half of the project takes place on Mars with organisms synthetically modified organisms who’ve had their circadian rhythms optimized to a Mar’s 25 hour day. The aim of this project is to display it in areas of Boston with various groups of people with different ideas and knowledge of synthetic biology. We want to get their opinions of the art anonymously to understand how far people are comfortable with the usage of synthetic modification in unusual situations.
Outreach: Summer Pathways
In June, we partnered with STEM Pathways and the BostonU Hardware team to host Summer Pathways, a synthetic biology Workshop for high school girls interested in STEM fields. We organized and led four interactive activities to introduce them to synthetic biology.
Our activities included a bioethics forum, a plasmid design activity, a gel electrophoresis with foody dye activity, and a microfluidic design activity. The bioethics forum was led by Steve, Sophia, and Manu. They held a fishbowl discussion about controversial issues in synthetic biology such as CRISPR and germline gene editing. The forum allowed us to hear opinions about synbio from those outside the field.
Thomas and Jason, a member of STEM Pathways, led a plasmid design activity in which they introduced the girls to plasmids, primers, and restriction enzymes. After the participants constructed plasmids using construction paper and scissors, Thomas and Jason demoed Benchling, the software we use to design plasmids in our lab.
The third station, gel electrophoresis with food dye, was adapted from William and Mary's 2013 Synthetic Biology K-12 curriculum. In this activity, Abbey, Sai, and Madeline discussed the basic biology and protocol of gel electrophoresis. Each participant then loaded a sample of food dye into a gel with a disposable pipette. The final activity, microfluidic design, was led by the BostonU Hardware team. The team first gave a basic overview of what microfluidics are and what applications they have in synbio. The participants were then given a basic protocol for E. coli transformation and challenged to design their own microfluidic chip on cardboard to perform the procedure.
Industry Visits
In August, we visited Ginkgo Bioworks in Boston's Seaport District. We toured their lab facilities, and were impressed by their automated workflow. We were shown some projects relating to synthetic fragrances and toured the workbench of Natzai Audrey Chieza, Ginkgo's current artist-in-residence, who uses bacteria to dye fabrics. Afterwards, we had a discussion with Ginkgo's creative director Christina Agapakis about Ginkgo Bioworks' interfaces of art and synthetic biology, and her experiences collaborating with artists and creating topical art herself. Our discussion inspired us to approach art that interfaced with the future of synthetic biology as the mainstay our human practices project.
We also visited the Fraunhofer Center for Manufacturing Innovation with BostonU_Hardware to inform our collaboration towards a microfluidic platform for RNA detection. They provided us with a wealth of knowledge about the considerations needed to translate a biological protocol to a microfluidic device at scale. More information about this collaboration can be found here.
