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<h1>INTERLAB STUDY</h1> | <h1>INTERLAB STUDY</h1> |
Revision as of 03:50, 29 October 2017
INTERLAB STUDY
We participated in the Fourth International InterLab Measurement Study. They asked us to measure the fluorescence of GFP in different devices in an attempt to explore the question, “How close can the numbers be when fluorescence is measured all around the world? “.
We followed the same protocols as directed in the iGEM protocol page.
Materials
E.coli DH5-alpha: Competent cells were purchased from TOYOBO
Plate reader: SH-9000Lab
Result
1. Calculation
LUDOX-HS40 | H2O | |
Replicate1 | 0.034 | 0.025 |
---|---|---|
Replicate2 | 0.031 | 0.024 |
Replicate3 | 0.032 | 0.025 |
Replicate4 | 0.031 | 0.023 |
Arith.Mean | 0.032 | 0.023 |
Corrected Abs600 | 0.00775 | |
Reference OD600 | 0.0425 | |
OD600/Abs600 | 5.483871 |
2. FIuorescein fluorescence standard curve
Table.2 shows the fluorescence measurement data, and Fig.1 shows the standard curve. We couldn’t measure the value of fluorescein fluorescence at 50, 25, 12.5, 6.25, 3.125 μM because they exceeded the measurable range.
uM Fluorescein | 1.5625 | 0.78125 | 0.390625 | 0.195313 | 0.097656 | 0.048828 | 0 |
---|---|---|---|---|---|---|---|
Replicate 1 | 57809 | 29980 | 15540 | 7861 | 3913 | 1949 | 3 |
Replicate 2 | 58424 | 30336 | 15369 | 7579 | 3948 | 1993 | 3 |
Replicate 3 | 58075 | 29993 | 15535 | 7723 | 3908 | 1999 | 2 |
Replicate 4 | 57953 | 29694 | 15532 | 7798 | 3935 | 2044 | 4 |
Arith. Mean | 58065.25 | 30000.75 | 15494 | 7740.25 | 3926 | 1996.25 | 3 |
S.D. | 262.7171 | 262.665 | 83.39864 | 121.4012 | 18.77942 | 38.86194 | 0.816497 |
3. Cell measurement
0h | 2h | 4h | 6h | |
---|---|---|---|---|
Negative Control (Colony 1) | 0.00475 | 0.0275 | 0.14125 | 0.25875 |
Negative Control (Colony 2) | 0.00975 | 0.0315 | 0.13275 | 0.235 |
Positive Control (Colony 1) | 0.007 | 0.01475 | 0.12125 | 0.25875 |
Positive Control (Colony 2) | 0.00825 | 0.019 | 0.1235 | 0.26925 |
Device 1 (Colony 1) | 0.0045 | 0.00375 | 0.00875 | 0.01 |
Device 1 (Colony 2) | 0.0045 | 0.00325 | 0.009 | 0.007 |
Device 2 (Colony 1) | 0.006 | 0.01825 | 0.12825 | 0.2665 |
Device 2 (Colony 2) | 0.00925 | 0.02325 | 0.141 | 0.26375 |
Device 3 (Colony 1) | 0.0065 | 0.02225 | 0.13425 | 0.2735 |
Device 3 (Colony 2) | 0.0105 | 0.0275 | 0.144 | 0.26325 |
Device 4 (Colony 1) | 0.00375 | 0.00325 | 0.00875 | 0.0445 |
Device 4 (Colony 2) | 0.0065 | 0.004 | 0.0085 | 0.0125 |
Device 5 (Colony 1) | 0.00575 | 0.02375 | 0.14775 | 0.28175 |
Device 5 (Colony 2) | 0.00875 | 0.019 | 0.1275 | 0.27675 |
Device 6 (Colony 1) | 0.006 | 0.02425 | 0.1505 | 0.2855 |
Device 6 (Colony 2) | 0.0085 | 0.02125 | 0.1005 | 0.2625 |
0h | 2h | 4h | 6h | |
---|---|---|---|---|
Negative Control (Colony 1) | 39.5 | 26.25 | 123 | 79 |
Negative Control (Colony 2) | 34.5 | 15 | 120 | 112 |
Positive Control (Colony 1) | 72.5 | 316.25 | 1272.75 | 2534.25 |
Positive Control (Colony 2) | 68.25 | 318.25 | 1269.25 | 2440.25 |
Device 1 (Colony 1) | 557.5 | 563 | 940.5 | 1046.5 |
Device 1 (Colony 2) | 461.75 | 355.25 | 633.75 | 781.25 |
Device 2 (Colony 1) | 83.75 | 374.75 | 2280.25 | 5128.5 |
Device 2 (Colony 2) | 116.25 | 494.25 | 2128.75 | 5528.5 |
Device 3 (Colony 1) | 3 | 29.5 | 154.75 | 232.75 |
Device 3 (Colony 2) | 28.5 | 29.75 | 161 | 258.5 |
Device 4 (Colony 1) | 94 | 172.5 | 327.75 | 769 |
Device 4 (Colony 2) | 71.75 | 165.5 | 286 | 443.5 |
Device 5 (Colony 1) | 7.5 | 196 | 614.5 | 858.25 |
Device 5 (Colony 2) | 14.5 | 189.75 | 796.75 | 987 |
Device 6 (Colony 1) | 60.25 | 29.5 | 129.5 | 164 |
Device 6 (Colony 2) | 33.75 | 26.5 | 97.75 | 165 |
0h | 2h | 4h | 6h | |
---|---|---|---|---|
Negative Control (Colony 1) | 0.045689 | 0.00469 | 0.004282 | 0.001499 |
Negative Control (Colony 2) | 0.018247 | 0.002376 | 0.004481 | 0.002352 |
Positive Control (Colony 1) | 0.055533 | 0.105486 | 0.051542 | 0.048113 |
Positive Control (Colony 2) | 0.04131 | 0.082437 | 0.050482 | 0.044579 |
Device 1 (Colony 1) | 0.607923 | 0.745125 | 0.532061 | 0.520307 |
Device 1 (Colony 2) | 0.522086 | 0.640948 | 0.355477 | 0.553925 |
Device 2 (Colony 1) | 0.070761 | 0.101285 | 0.087415 | 0.094541 |
Device 2 (Colony 2) | 0.063885 | 0.104249 | 0.074675 | 0.103012 |
Device 3 (Colony 1) | 0.001716 | 0.006526 | 0.005688 | 0.004194 |
Device 3 (Colony 2) | 0.01537 | 0.005312 | 0.005489 | 0.004827 |
Device 4 (Colony 1) | 0.118182 | 0.265189 | 0.184017 | 0.084842 |
Device 4 (Colony 2) | 0.054166 | 0.218167 | 0.166671 | 0.177319 |
Device 5 (Colony 1) | 0.00281 | 0.040555 | 0.020409 | 0.014984 |
Device 5 (Colony 2) | 0.007484 | 0.049747 | 0.030945 | 0.017517 |
Device 6 (Colony 1) | 0.050096 | 0.005969 | 0.004225 | 0.002821 |
Device 6 (Colony 2) | 0.024278 | 0.006149 | 0.004835 | 0.003078 |