Difference between revisions of "Team:CIEI-China/Notebook/Lab Book"

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<ul class="page-anchors">
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<li><a href="#a1">Jan. 2017</a>
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<li><a href="#a2">Mar. 2017</a>
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<li><a href="#a3">Apr.2017</a>
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<li><a href="#a4">May. 2017</a>
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<li><a href="#a5">Jun. 2017</a>
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<li><a href="#a6">Week 1-2 in Jul. 2017</a>
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<li><a href="#a7">Week 3 in Jul. 2017</a>
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<li><a href="#a8">Week 4 in Jul. 2017 to week 1 in Aug. 2017</a>
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<li><a href="#a9">Week 2 –week 3 in Aug.2017</a>
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<li><a href="#a10">Week 4 in Aug. 2017 to week 1 in Sept. 2017</a>
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<li><a href="#a11">Week 2-4 in Sep. 2017</a>
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<li><a href="#a12">Week 1-3 in Oct. 2017</a>
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<div class="first-level" id="a1"  >Jan. 2017</div>
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<p class="my-content" >We came up with the idea about using salt tolerant yeast to dispose kitchen waste which have high osmotic pressure. Then we started to search the papers related to our research.</p>
 +
<div class="first-level" id="a2"  >Mar. 2017</div>
 +
<p class="my-content" >We found three genes that are possible for our goal: <i>SpTPS1</i>, <i>ScTPS1</i>, and <i>gltB</i>.</p>
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<div class="first-level" id="a3"  >Apr.2017</div>
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<p class="my-content" >And then we began to prepare the materials we need in the following experiments.</p>
 +
<div class="first-level" id="a4"  >May. 2017</div>
 +
<p class="my-content" >We finished the design of nine biobricks with different functions.</p>
 +
<div class="first-level" id="a5"  >Jun. 2017</div>
 +
<p class="my-content" >Designing and editing of the experiment plan.</p>
 +
<p class="my-content" >Transformation of PUC57 plasmid into <i>DH5α</i>.</p>
 +
<p class="my-content" >Culture the <i>E.coli</i>.</p>
 +
<p class="my-content" >Preparation of plasmid DNA by Alkaline Lysis with SDS;</p>
 +
<p class="my-content" >Digestion (<i>Xba</i>Ⅰ, <i>Spe</i>Ⅰ).</p>
 +
<p class="my-content" >Recycle the electrophoresis product.</p>
 +
<p class="my-content" >Linearization of the standard carrier.</p>
 +
<div class="first-level" id="a6"  >Week 1-2 in Jul. 2017</div>
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<p class="my-content" >Ligate the genes into the standard carrier.</p>
 +
<p class="my-content" >Transform the standard carrier into <i>DH5α E.coli</i>;</p>
 +
<p class="my-content" >Culture the <i>E.coli</i> and prepare the plasmid.</p>
 +
<div class="first-level" id="a7"  >Week 3 in Jul. 2017</div>
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<p class="my-content" >Design the primers of the three genes: <i>SpTPS1</i>, <i>ScTPS1</i>, and <i>gltB</i>.</p>
 +
<div class="first-level" id="a8"  >Week 4 in Jul. 2017 to week 1 in Aug. 2017</div>
 +
<p class="my-content" >PCR PrimeStar DNA polymerase.</p>
 +
<p class="my-content" >Recycle the plasmids from PCR products.</p>
 +
<p class="my-content" >Digest the PCR products.</p>
 +
<p class="my-content" >Electrophoresis and recycling.</p>
 +
<div class="first-level" id="a9"  >Week 2 –week 3 in Aug.2017</div>
 +
<p class="my-content" >Ligation;</p>
 +
<p class="my-content" >We successfully ligated the three target genes in to the vector pPIC9K to get the devices.</p>
 +
<p class="my-content" >Transform the vector into <i>DH5α E.coli</i>.</p>
 +
<p class="my-content" >Culture the <i>E.coli</i>.</p>
 +
<p class="my-content" >Extract the devices.</p>
 +
<div class="first-level" id="a10"  >Week 4 in Aug. 2017 to week 1 in Sept. 2017</div>
 +
<p class="my-content" >Electrophoresis of six completed devices: intracellular and extracellular expression of the three target genes. Recycle the devices from the gels.</p>
 +
<p class="my-content" >Transform the devices into <i>GS115</i> competent yeast cells.</p>
 +
<div class="first-level" id="a11"  >Week 2-4 in Sep. 2017</div>
 +
<p class="my-content" >Sequencing the genes we transformed into the yeast.</p>
 +
<p class="my-content" >Induce experiments by using methanol.</p>
 +
<p class="my-content" >SDS-Page.</p>
 +
<div class="first-level" id="a12"  >Week 1-3 in Oct. 2017</div>
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<p class="my-content" >Test the survive rate of the yeast in different salinity.</p>
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<p class="my-content" >Test the decomposition rate of the waste.</p>
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    <h1 class="text-center title">Lab Book</h1><br />
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    <h3>Jan. 2017</h3>
 
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    <p>
 
−
    We came up with the idea about using salt tolerant yeast to dispose kitchen waste which have high osmotic pressure. Then we started to search the papers related to our research.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Mar. 2017</h3>
 
−
 
−
    <p>
 
−
    We found three genes that are possible for our goal: <i>SpTPS1</i>, <i>ScTPS1</i>, and <i>gltB</i>.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Apr.2017</h3>
 
−
 
−
    <p>
 
−
    And then we began to prepare the materials we need in the following experiments.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>May. 2017</h3>
 
−
 
−
    <p>
 
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    We finished the design of nine biobricks with different functions.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Jun. 2017</h3>
 
−
 
−
    <p>
 
−
    Designing and editing of the experiment plan.
 
−
<br />Transformation of PUC57 plasmid into <i>DH5α</i>.
 
−
<br />Culture the <i>E.coli</i>.
 
−
<br />Preparation of plasmid DNA by Alkaline Lysis with SDS;
 
−
<br />Digestion (<i>Xba</i>Ⅰ, <i>Spe</i>Ⅰ).
 
−
<br />Recycle the electrophoresis product.
 
−
<br />Linearization of the standard carrier.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Week 1-2 in Jul. 2017</h3>
 
−
 
−
    <p>
 
−
    Ligate the genes into the standard carrier.
 
−
<br />Transform the standard carrier into <i>DH5α E.coli</i>;
 
−
<br />Culture the <i>E.coli</i> and prepare the plasmid.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Week 3 in Jul. 2017</h3>
 
−
 
−
    <p>
 
−
    Design the primers of the three genes: <i>SpTPS1</i>, <i>ScTPS1</i>, and <i>gltB</i>.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Week 4 in Jul. 2017 to week 1 in Aug. 2017</h3>
 
−
 
−
    <p>
 
−
    PCR PrimeStar DNA polymerase.
 
−
<br />Recycle the plasmids from PCR products.
 
−
<br />Digest the PCR products.
 
−
<br />Electrophoresis and recycling.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Week 2 –week 3 in Aug.2017</h3>
 
−
 
−
    <p>
 
−
    Ligation;
 
−
<br />We successfully ligated the three target genes in to the vector pPIC9K to get the devices.
 
−
<br />Transform the vector into <i>DH5α E.coli</i>.
 
−
<br />Culture the <i>E.coli</i>.
 
−
<br />Extract the devices.
 
−
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Week 4 in Aug. 2017 to week 1 in Sept. 2017</h3>
 
−
 
−
    <p>
 
−
    Electrophoresis of six completed devices: intracellular and extracellular expression of the three target genes. Recycle the devices from the gels.
 
−
Transform the devices into <i>GS115</i> competent yeast cells.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Week 2-4 in Sep. 2017</h3>
 
−
 
−
    <p>
 
−
    Sequencing the genes we transformed into the yeast.
 
−
<br />Induce experiments by using methanol.
 
−
<br />SDS-Page.
 
−
    </p>
 
−
 
−
    <br />
 
−
 
−
    <h3>Week 1-3 in Oct. 2017</h3>
 
−
 
−
    <p>
 
−
    Test the survive rate of the yeast in different salinity.
 
−
<br />Test the decomposition rate of the waste.
 
−
    </p>
 
−
   
 
−
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Revision as of 04:00, 29 October 2017

Bootstrap 101 Template  

Jan. 2017

We came up with the idea about using salt tolerant yeast to dispose kitchen waste which have high osmotic pressure. Then we started to search the papers related to our research.

Mar. 2017

We found three genes that are possible for our goal: SpTPS1, ScTPS1, and gltB.

Apr.2017

And then we began to prepare the materials we need in the following experiments.

May. 2017

We finished the design of nine biobricks with different functions.

Jun. 2017

Designing and editing of the experiment plan.

Transformation of PUC57 plasmid into DH5α.

Culture the E.coli.

Preparation of plasmid DNA by Alkaline Lysis with SDS;

Digestion (XbaⅠ, SpeⅠ).

Recycle the electrophoresis product.

Linearization of the standard carrier.

Week 1-2 in Jul. 2017

Ligate the genes into the standard carrier.

Transform the standard carrier into DH5α E.coli;

Culture the E.coli and prepare the plasmid.

Week 3 in Jul. 2017

Design the primers of the three genes: SpTPS1, ScTPS1, and gltB.

Week 4 in Jul. 2017 to week 1 in Aug. 2017

PCR PrimeStar DNA polymerase.

Recycle the plasmids from PCR products.

Digest the PCR products.

Electrophoresis and recycling.

Week 2 –week 3 in Aug.2017

Ligation;

We successfully ligated the three target genes in to the vector pPIC9K to get the devices.

Transform the vector into DH5α E.coli.

Culture the E.coli.

Extract the devices.

Week 4 in Aug. 2017 to week 1 in Sept. 2017

Electrophoresis of six completed devices: intracellular and extracellular expression of the three target genes. Recycle the devices from the gels.

Transform the devices into GS115 competent yeast cells.

Week 2-4 in Sep. 2017

Sequencing the genes we transformed into the yeast.

Induce experiments by using methanol.

SDS-Page.

Week 1-3 in Oct. 2017

Test the survive rate of the yeast in different salinity.

Test the decomposition rate of the waste.